Construct: ORF TRCN0000473124
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009936.1_s317c1
- Derived from:
- ccsbBroadEn_06386
- DNA Barcode:
- TTGAGCATGCCAGGCCTTCGGCCT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- HNMT (3176)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473124
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3176 | HNMT | histamine N-methyltransferase | NM_006895.3 | 99.8% | 99.6% | 700A>G |
| 2 | human | 3176 | HNMT | histamine N-methyltransferase | XM_017003948.1 | 85.9% | 84.2% | (many diffs) |
| 3 | human | 3176 | HNMT | histamine N-methyltransferase | XM_011511064.2 | 56.7% | 56.5% | 0_1ins378;322A>G |
| 4 | human | 3176 | HNMT | histamine N-methyltransferase | XM_017003949.2 | 53.7% | 48.3% | (many diffs) |
| 5 | human | 3176 | HNMT | histamine N-methyltransferase | NM_001024075.2 | 34.8% | 25.8% | (many diffs) |
| 6 | human | 3176 | HNMT | histamine N-methyltransferase | XR_002959286.1 | 25% | (many diffs) | |
| 7 | human | 3176 | HNMT | histamine N-methyltransferase | NM_001024074.3 | 16.5% | 15.7% | (many diffs) |
| 8 | mouse | 140483 | Hnmt | histamine N-methyltransferase | NM_080462.2 | 86.2% | 82.7% | (many diffs) |
| 9 | mouse | 140483 | Hnmt | histamine N-methyltransferase | XM_006497671.3 | 86.2% | 82.7% | (many diffs) |
| 10 | mouse | 140483 | Hnmt | histamine N-methyltransferase | XM_006497670.3 | 82.8% | 79.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 942
- ORF length:
- 876
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc atcttccatg aggagcttgt tttctgacca cgggaaatat gttgaatctt 121 tccggaggtt tctcaaccat tccacggaac accagtgcat gcaggaattc atggacaaga 181 agctgccagg cataatagga aggattggag acacaaaatc agaaattaag attctaagca 241 taggcggagg tgcaggtgaa attgatcttc aaattctctc caaagttcag gctcaatacc 301 caggagtttg tatcaacaat gaagttgttg agccaagtgc tgaacaaatt gccaaataca 361 aagagcttgt agccaagaca tcgaacctcg agaacgtaaa gtttgcttgg cataaggaga 421 catcatctga ataccaaagt agaatgttgg agaaaaagga gcttcaaaag tgggacttta 481 ttcatatgat tcaaatgctg tattatgtaa aagacatccc agctaccctg aaatTCTTCC 541 ATAGTCTCTT AGGTACCAAT GCTAAGATGC TCATTATTGT TGTGTCAGGA AGCAGTGGCT 601 GGGACAAGCT GTGGAAAAAG TACGGATCAC GCTTTCCCCA GGATGACCTC TGCCAGTATA 661 TCACATCAGA TGACCTCACT CAGATGCTGG ACAACCTAGG GCTTAAGTAT GAGTGCTATG 721 ACCTTTTGTC CACCATGGAT ATATCTGACT GCTTTATTGA TGGTGATGAA AATGGAGACC 781 TGCTTTGGGA TTTTTTGACT GAAACCTGCA ACTTTAATGC CACAGCACCA CCTGATCTCA 841 GAGCAGAGCT TGGGAAAGAT CTACAAGAGC CTGAATTTAG TGCTAAGAAA GAGGGGAAGG 901 TTCTTTTTAA TAATACTCTG AGTTTCATAG TGATTGAGGC ATACCCAACT TTCTTGTACA 961 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 1021 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 1081 AGGACGATTG AGCATGCCAG GCCTTCGGCC TACGCGTTAA GTCgacaatc aacctctgga 1141 ttacaaaatt tgtgaaagat t