Construct: ORF TRCN0000473500

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF013831.1_s317c1
Derived from:
ccsbBroadEn_15448
DNA Barcode:
ATTCCCCAGCCCCATTGAGTCACT
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
HNMT (3176)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000473500
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 3176 HNMT histamine N-methyltransferase NM_001024074.3 100% 100%
2 human 3176 HNMT histamine N-methyltransferase NM_001024075.2 38.2% 36.5% (many diffs)
3 human 3176 HNMT histamine N-methyltransferase XM_017003949.2 29.9% 28.5% (many diffs)
4 human 3176 HNMT histamine N-methyltransferase NM_006895.3 16.5% 15.7% (many diffs)
5 human 3176 HNMT histamine N-methyltransferase XR_002959286.1 4.3% 1_97del;251_3490del
6 mouse 140483 Hnmt histamine N-methyltransferase NM_080462.2 15.1% 13.8% (many diffs)
7 mouse 140483 Hnmt histamine N-methyltransferase XM_006497671.3 15.1% 13.8% (many diffs)
8 mouse 140483 Hnmt histamine N-methyltransferase XM_006497670.3 14.5% 13.3% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
314
ORF end:
467
ORF length:
153
Sequence:
1GTCGCTTCAT GTGACTCCAC GGAGTACCGG GCGCCGTCCA GGCACCTCGA TTAGTTCTCG
61AGCTTTTGGA GTACGTCGTC TTTAGGTTGG GGGGAGGGGT TTTATGCGAT GGAGTTTCCC
121CACACTGAGT GGGTGGAGAC TGAAGTTAGG CCAGCTTGGC ACTTGATGTA ATTCTCCTTG
181GAATTTGCCC TTTTTGAGTT TGGATCTTGG TTCATTCTCA AGCCTCAGAC AGTGGTTCAA
241AGTTTTTTTC TTCCATTTCA GGTGTCGTGA GGCTAGCATC GATTGATCAA CAAGTTTGTA
301CAAAAAAGTT GGCATGGCAT CTTCCATGAG GAGCTTGTTT TCTGACCACG GGAAATATGT
361TGAATCTTTC CGGAGGTTTC TCAACCATTC CACGGAACAC CAGTGCATGC AGGAATTCAT
421GGACAAGAAG CTGCCAGGCA TAATAGGAAG ATACCAGAAT TGCTGTTACC CAACTTTCTT
481GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC
541GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG
601TGGAAAGGAC GAATTCCCCA GCCCCATTGA GTCACTACGC GTTAAGTCga caatcaacct
661ctggattaca aaatttgtga aagatt

Download FASTA (ORF) (Full)