Construct: ORF TRCN0000473540
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006730.1_s317c1
- Derived from:
- ccsbBroadEn_14856
- DNA Barcode:
- CTTAATAACGAGGATTAACAACGT
- Epitope Tag:
- V5 (not translated due to frame shift)
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MAP3K7 (6885)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473540
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6885 | MAP3K7 | mitogen-activated protein k... | NM_003188.4 | 100% | 100% | |
| 2 | human | 6885 | MAP3K7 | mitogen-activated protein k... | NM_145331.3 | 95.5% | 95.5% | 1208_1288del |
| 3 | human | 6885 | MAP3K7 | mitogen-activated protein k... | NM_145333.3 | 84.8% | 82.9% | 1443_1444ins116;1473_1474ins148 |
| 4 | human | 6885 | MAP3K7 | mitogen-activated protein k... | NM_145332.3 | 81% | 79.3% | 1208_1288del;1524_1525ins116;1554_1555ins148 |
| 5 | human | 6885 | MAP3K7 | mitogen-activated protein k... | XM_017011226.2 | 77.5% | 77.5% | 0_1ins390 |
| 6 | human | 6885 | MAP3K7 | mitogen-activated protein k... | XM_006715553.3 | 74% | 74% | 0_1ins390;818_898del |
| 7 | mouse | 26409 | Map3k7 | mitogen-activated protein k... | NM_172688.3 | 91.7% | 99.3% | (many diffs) |
| 8 | mouse | 26409 | Map3k7 | mitogen-activated protein k... | NM_009316.1 | 87.8% | 94.8% | (many diffs) |
| 9 | mouse | 26409 | Map3k7 | mitogen-activated protein k... | XM_006537948.3 | 68.5% | 73.5% | (many diffs) |
| 10 | mouse | 26409 | Map3k7 | mitogen-activated protein k... | XR_390323.3 | 24.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1803
- ORF length:
- 1737
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc tacagcctct gccgcctcct cctcctcctc gtcttcggcc ggtgagatga 121 tcgaagcccc ttcccaggtc ctcaactttg aagagatcga ctacaaggag atcgaggtgg 181 aagaggttgt tggaagagga gcctttggag ttgtttgcaa agctaagtgg agagcaaaag 241 atgttgctat taaacaaata gaaagtgaat ctgagaggaa agcgtttatt gtagagcttc 301 ggcagttatc ccgtgtgaac catcctaata ttgtaaagct ttatggagcc tgcttgaatc 361 cagtgtgtct tgtgatggaa tatgctgaag ggggctcttt atataatgtg ctgcatggtg 421 ctgaaccatt gccatattat actgctgccc acgcaatgag ttggtgttta cagtgttccc 481 aaggagtggc ttatcttcac agcatgcaac ccaaagcgct aattcacagg gacctgaaac 541 caccaaactt actgctggtt gcagggggga cagttctaaa aatttgtgat tttggtacag 601 cctgtgacat tcagacacac atgaccaata acaaggggag tgctgcttgg atggcacctg 661 aagtttttga aggtagtaat tacagtgaaa aatgtgacgt cttcagctgg ggtattattc 721 tttgggaagt gataacgcgt cggaaaccct ttgatgagat tggtggccca gctttccgaa 781 tcatgtgggc tgttcataat ggtactcgac caccactgat aaaaaattta cctaagccca 841 ttgagagcct gatgactcgt tgttggtcta aagatccttc ccagcgccct tcaatggagg 901 aaattgtgaa aataatgact cacttgatgc ggtactttcc aggagcagat gagccattac 961 agtatccttg tcagtattca gatgaaggac agagcaactc tgccaccagt acaggctcat 1021 tcatggacat tgcttctaca aatacgagta acaaaagtga cactaatatg gagcaagttc 1081 ctgccacaaa tgatactatt aagcgcttag aatcaaaatt gttgaaaaat caggcaaagc 1141 aacagagtga atctggacgt ttaagcttgg gagcctcccg tgggagcagt gtggagagct 1201 tgcccccaac ctctgagggc aagaggatga gtgctgacat gtctgaaata gaagctagga 1261 tcgccgcaac cacaggcaac ggacagccaa gacgtagatc catccaagac ttgactgtaa 1321 ctggaacaga acctggTCAG GTGAGCAGTA GGTCATCCAG TCCCAGTGTC AGAATGATTA 1381 CTACCTCAGG ACCAACCTCA GAAAAGCCAA CTCGAAGTCA TCCATGGACC CCTGATGATT 1441 CCACAGATAC CAATGGATCA GATAACTCCA TCCCAATGGC TTATCTTACA CTGGATCACC 1501 AACTACAGCC TCTAGCACCG TGCCCAAACT CCAAAGAATC TATGGCAGTG TTTGAACAGC 1561 ATTGTAAAAT GGCACAAGAA TATATGAAAG TTCAAACAGA AATTGCATTG TTATTACAGA 1621 GAAAGCAAGA ACTAGTTGCA GAACTGGACC AGGATGAAAA GGACCAGCAA AATACATCTC 1681 GCCTGGTACA GGAACATAAA AAGCTTTTAG ATGAAAACAA AAGCCTTTCT ACTTACTACC 1741 AGCAATGCAA AAAACAACTA GAGGTCATCA GAAGTCAGCA GCAGAAACGA CAAGGCACTT 1801 CATCCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT 1861 CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG 1921 GCTTTATATA TCTTGTGGAA AGGACGACTT AATAACGAGG ATTAACAACG TACGCGTTAA 1981 GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t