Construct: ORF TRCN0000473648
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008369.1_s317c1
- Derived from:
- ccsbBroadEn_04734
- DNA Barcode:
- CATCACTCCGAAACTTCTTTGTGG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SFXN2 (118980)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473648
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 118980 | SFXN2 | sideroflexin 2 | NM_001350989.1 | 100% | 100% | |
| 2 | human | 118980 | SFXN2 | sideroflexin 2 | NM_178858.6 | 100% | 100% | |
| 3 | human | 118980 | SFXN2 | sideroflexin 2 | XM_011539261.2 | 97.2% | 97.2% | 1_27del |
| 4 | human | 118980 | SFXN2 | sideroflexin 2 | XM_024447792.1 | 94.7% | 94.7% | 1_54del |
| 5 | human | 118980 | SFXN2 | sideroflexin 2 | XR_945599.3 | 75.4% | 1_16del;608_609ins128;757_758ins97 | |
| 6 | human | 118980 | SFXN2 | sideroflexin 2 | XM_011539263.2 | 65.2% | 65.2% | 0_1ins336 |
| 7 | human | 118980 | SFXN2 | sideroflexin 2 | XM_011539264.2 | 65.2% | 65.2% | 0_1ins336 |
| 8 | human | 118980 | SFXN2 | sideroflexin 2 | XM_017015663.1 | 49.4% | 41.9% | 1_54del;483_484ins76;558_559ins386 |
| 9 | human | 118980 | SFXN2 | sideroflexin 2 | XR_001747021.1 | 43.4% | 1_428del;857_858ins76;1034_1035ins284 | |
| 10 | human | 118980 | SFXN2 | sideroflexin 2 | NR_146992.1 | 28.6% | (many diffs) | |
| 11 | mouse | 94279 | Sfxn2 | sideroflexin 2 | NM_053196.3 | 86.4% | 89.1% | (many diffs) |
| 12 | mouse | 94279 | Sfxn2 | sideroflexin 2 | XR_386525.2 | 57.4% | (many diffs) | |
| 13 | mouse | 94279 | Sfxn2 | sideroflexin 2 | XM_017318316.1 | 50.1% | 47.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1032
- ORF length:
- 966
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga ggctgacctg tctggcttta acatcgatgc cccccgttgg gaccagcgca 121 ccttcctggg gagagtgaag cacttcctaa acatcacgga cccccgcact gtctttgtat 181 ctgagcggga gctggactgg gccaaggtga tggtggagaa gagcaggatg ggggttgtgc 241 ccccaggcac ccaagtggag cagctgctgt atgccaagaa gctgtatgac tcggccttcc 301 accccgacac tggggagaag atgaatgtca tcgggcgcat gtctttccag cttcctggcg 361 gcatgatcat cacgggcttc atgctccagt tctacaggac gatgccggcg gtgatcttct 421 ggcagtgggt gaaccagtcc ttcaatgcct tagtcaacta caccaacagg aatgcggctt 481 cccccacatc agtcaggcag atggcccttt cctacttcac agccacaacc actgctgtgg 541 ccacggctgt gggcatgaac atgttgacaa agaaagcgcc gcccttggtg ggccgctggg 601 tgCCCTTTGC CGCTGTGGCT GCGGCTAACT GTGTCAATAT CCCCATGATG CGACAGCAGG 661 AGCTCATAAA GGGAATCTGC GTGAAGGACA GGAATGAAAA TGAGATTGGT CATTCCCGGA 721 GAGCTGCGGC CATAGGCATC ACCCAAGTAG TTATTTCTCG GATCACCATG TCAGCTCCTG 781 GGATGATCTT GCTGCCAGTC ATCATGGAAA GGCTTGAGAA ATTGCACTTC ATGCAGAAAG 841 TCAAGGTCCT GCACGCCCCA TTGCAGGTCA TGCTGAGCGG GTGCTTCCTC ATCTTCATGG 901 TGCCAGTGGC GTGTGGGCTT TTCCCACAGA AATGTGAATT GCCAGTTTCC TATCTGGAAC 961 CGAAGCTCCA AGACACTATC AAGGCCAAGT ATGGAGAACT TGAGCCTTAT GTCTACTTCA 1021 ATAAGGGTCT CTACCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 1081 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 1141 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGACAT CACTCCGAAA CTTCTTTGTG 1201 GACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t