Transcript: Human NM_178858.6

Homo sapiens sideroflexin 2 (SFXN2), transcript variant 1, mRNA.

Source:
NCBI, updated 2019-09-27
Taxon:
Homo sapiens (human)
Gene:
SFXN2 (118980)
Length:
6770
CDS:
72..1040

Additional Resources:

NCBI RefSeq record:
NM_178858.6
NBCI Gene record:
SFXN2 (118980)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_178858.6, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000059484 GCATCACCCAAGTAGTTATTT pLKO.1 742 CDS 100% 15.000 21.000 N SFXN2 n/a
2 TRCN0000059486 TGCGGCTAACTGTGTCAATAT pLKO.1 626 CDS 100% 13.200 18.480 N SFXN2 n/a
3 TRCN0000436355 GAATTGCCAGTTTCCTATCTG pLKO_005 942 CDS 100% 4.950 6.930 N SFXN2 n/a
4 TRCN0000425044 GCATGATCATCACGGGCTTCA pLKO_005 367 CDS 100% 4.050 3.240 N SFXN2 n/a
5 TRCN0000419351 GGACCAGTCTATTCCCATATT pLKO_005 1058 3UTR 100% 13.200 9.240 N SFXN2 n/a
6 TRCN0000432405 CAATGAATGCCTCATACTTAC pLKO_005 1169 3UTR 100% 10.800 7.560 N SFXN2 n/a
7 TRCN0000059487 GAGAGTGAAGCACTTCCTAAA pLKO.1 137 CDS 100% 10.800 7.560 N SFXN2 n/a
8 TRCN0000059485 CTACTTCAATAAGGGTCTCTA pLKO.1 1019 CDS 100% 4.950 3.465 N SFXN2 n/a
9 TRCN0000417056 GATGATCTTGCTGCCAGTCAT pLKO_005 788 CDS 100% 4.950 3.465 N SFXN2 n/a
10 TRCN0000059483 GCTTGAGAAATTGCACTTCAT pLKO.1 818 CDS 100% 4.950 3.465 N SFXN2 n/a
11 TRCN0000060503 CCTCTCAAGTAGCTGGGATTA pLKO.1 1694 3UTR 100% 10.800 5.400 Y NCCRP1 n/a
12 TRCN0000155576 CCTCTCAAGTAGCTGGGATTA pLKO.1 1694 3UTR 100% 10.800 5.400 Y KLHL30 n/a
13 TRCN0000165697 CCTCTCAAGTAGCTGGGATTA pLKO.1 1694 3UTR 100% 10.800 5.400 Y SLC48A1 n/a
14 TRCN0000008902 CCTCCCAAAGTGTTGGGATTA pLKO.1 4937 3UTR 100% 1.080 0.540 Y GPR83 n/a
15 TRCN0000156315 CCTCCCAAAGTGTTGGGATTA pLKO.1 4937 3UTR 100% 1.080 0.540 Y MYORG n/a
16 TRCN0000155836 CCCAAAGTGCTGGGATTACAA pLKO.1 5696 3UTR 100% 5.625 2.813 Y KLHL30 n/a
17 TRCN0000141025 CCCAAAGTGCTGGGATTACTT pLKO.1 5696 3UTR 100% 5.625 2.813 Y EID2B n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_178858.6, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_04734 pDONR223 100% 100% 100% None n/a
2 ccsbBroad304_04734 pLX_304 0% 100% 100% V5 n/a
3 TRCN0000473648 CATCACTCCGAAACTTCTTTGTGG pLX_317 44.6% 100% 100% V5 n/a
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