Construct: ORF TRCN0000473671
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011665.1_s317c1
- Derived from:
- ccsbBroadEn_07462
- DNA Barcode:
- ATACATCCAACTTCAGTAGTATCG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PDE4DIP (9659)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473671
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9659 | PDE4DIP | phosphodiesterase 4D intera... | NM_001002811.2 | 100% | 100% | |
| 2 | human | 9659 | PDE4DIP | phosphodiesterase 4D intera... | NM_001002812.2 | 79% | 67.3% | (many diffs) |
| 3 | human | 9659 | PDE4DIP | phosphodiesterase 4D intera... | XM_017002901.2 | 50.1% | 50.1% | 3396_6771delinsA |
| 4 | human | 9659 | PDE4DIP | phosphodiesterase 4D intera... | XM_017002900.2 | 49.7% | 49.8% | 3396_6819delinsA |
| 5 | human | 9659 | PDE4DIP | phosphodiesterase 4D intera... | XM_017002897.2 | 47.8% | 47.8% | 3396_7098delinsA |
| 6 | human | 9659 | PDE4DIP | phosphodiesterase 4D intera... | XM_017002896.2 | 47.8% | 47.8% | 3396_7101delinsA |
| 7 | human | 9659 | PDE4DIP | phosphodiesterase 4D intera... | XM_017002890.2 | 47.4% | 47.5% | 3396_7149delinsA |
| 8 | human | 9659 | PDE4DIP | phosphodiesterase 4D intera... | XM_017002886.2 | 47.1% | 47.2% | 3396_7194delinsA |
| 9 | human | 9659 | PDE4DIP | phosphodiesterase 4D intera... | XM_017002885.2 | 47.1% | 47.1% | 3396_7197delinsA |
| 10 | human | 9659 | PDE4DIP | phosphodiesterase 4D intera... | XM_017002884.2 | 46.5% | 46.6% | 3396_7287delinsA |
| 11 | human | 9659 | PDE4DIP | phosphodiesterase 4D intera... | XM_017002883.2 | 46.2% | 46.2% | 3396_7335delinsA |
| 12 | human | 9659 | PDE4DIP | phosphodiesterase 4D intera... | XM_017002882.2 | 46.2% | 46.2% | 3396_7338delinsA |
| 13 | human | 9659 | PDE4DIP | phosphodiesterase 4D intera... | XM_017002881.2 | 46.2% | 46.2% | 3396_7341delinsA |
| 14 | human | 9659 | PDE4DIP | phosphodiesterase 4D intera... | XM_017002879.2 | 45.9% | 45.9% | 3396_7389delinsA |
| 15 | human | 9659 | PDE4DIP | phosphodiesterase 4D intera... | XM_006711648.3 | 45.5% | 45.6% | 3396_7446delinsA |
| 16 | human | 9659 | PDE4DIP | phosphodiesterase 4D intera... | NM_001350520.1 | 45.1% | 45.1% | 3396_7524delinsA |
| 17 | human | 9659 | PDE4DIP | phosphodiesterase 4D intera... | XM_005272981.4 | 45.1% | 45.1% | 3396_7527delinsA |
| 18 | human | 9659 | PDE4DIP | phosphodiesterase 4D intera... | XM_011510174.2 | 44.7% | 44.7% | 3396_7590delinsA |
| 19 | human | 9659 | PDE4DIP | phosphodiesterase 4D intera... | XM_017002876.2 | 44.5% | 44.5% | 3396_7626delinsA |
| 20 | human | 9659 | PDE4DIP | phosphodiesterase 4D intera... | XM_017002875.2 | 44.4% | 44.4% | 3396_7635delinsA |
| 21 | human | 9659 | PDE4DIP | phosphodiesterase 4D intera... | XM_005277442.5 | 43.8% | 43.8% | 3396_7743delinsA |
| 22 | human | 9659 | PDE4DIP | phosphodiesterase 4D intera... | XM_006711643.3 | 43.6% | 43.6% | 3396_7773delinsA |
| 23 | human | 9659 | PDE4DIP | phosphodiesterase 4D intera... | XM_006711642.3 | 43.6% | 43.6% | 3396_7776delinsA |
| 24 | human | 9659 | PDE4DIP | phosphodiesterase 4D intera... | XR_001737551.2 | 42.2% | 1_407del;3803_8036delinsA | |
| 25 | human | 9659 | PDE4DIP | phosphodiesterase 4D intera... | XM_011510176.2 | 39.5% | 32.9% | (many diffs) |
| 26 | human | 9659 | PDE4DIP | phosphodiesterase 4D intera... | XM_024451068.1 | 39.3% | 32.7% | (many diffs) |
| 27 | human | 9659 | PDE4DIP | phosphodiesterase 4D intera... | NM_001198832.2 | 39.1% | 32.7% | (many diffs) |
| 28 | human | 9659 | PDE4DIP | phosphodiesterase 4D intera... | XM_017002903.2 | 39.1% | 32.7% | (many diffs) |
| 29 | human | 9659 | PDE4DIP | phosphodiesterase 4D intera... | NM_001350523.1 | 38.9% | 32.5% | (many diffs) |
| 30 | human | 9659 | PDE4DIP | phosphodiesterase 4D intera... | XM_017002899.2 | 38.2% | 31.9% | (many diffs) |
| 31 | human | 9659 | PDE4DIP | phosphodiesterase 4D intera... | XM_024451067.1 | 37.9% | 31.6% | (many diffs) |
| 32 | human | 9659 | PDE4DIP | phosphodiesterase 4D intera... | NM_001350522.1 | 37.5% | 31.4% | (many diffs) |
| 33 | human | 9659 | PDE4DIP | phosphodiesterase 4D intera... | NM_001350521.2 | 37.2% | 31% | (many diffs) |
| 34 | human | 9659 | PDE4DIP | phosphodiesterase 4D intera... | XM_011510175.2 | 36.9% | 30.8% | (many diffs) |
| 35 | human | 9659 | PDE4DIP | phosphodiesterase 4D intera... | XM_017002878.2 | 36.9% | 30.8% | (many diffs) |
| 36 | human | 9659 | PDE4DIP | phosphodiesterase 4D intera... | XM_011510179.1 | 35.9% | 31% | (many diffs) |
| 37 | human | 9659 | PDE4DIP | phosphodiesterase 4D intera... | NM_014644.5 | 35.9% | 31% | (many diffs) |
| 38 | human | 9659 | PDE4DIP | phosphodiesterase 4D intera... | XM_011510173.2 | 35.8% | 29.9% | (many diffs) |
| 39 | human | 9659 | PDE4DIP | phosphodiesterase 4D intera... | XM_011510172.2 | 35.8% | 29.8% | (many diffs) |
| 40 | human | 9659 | PDE4DIP | phosphodiesterase 4D intera... | NM_001198834.3 | 35.6% | 30.8% | (many diffs) |
| 41 | human | 9659 | PDE4DIP | phosphodiesterase 4D intera... | XM_006711646.2 | 35.4% | 29.6% | (many diffs) |
| 42 | human | 9659 | PDE4DIP | phosphodiesterase 4D intera... | XM_006711652.1 | 34.7% | 30.1% | (many diffs) |
| 43 | human | 9659 | PDE4DIP | phosphodiesterase 4D intera... | XM_006711651.1 | 34.7% | 30.1% | (many diffs) |
| 44 | human | 9659 | PDE4DIP | phosphodiesterase 4D intera... | XM_006711650.4 | 34.3% | 30.3% | (many diffs) |
| 45 | human | 9659 | PDE4DIP | phosphodiesterase 4D intera... | XM_017002887.1 | 34.1% | 29.9% | (many diffs) |
| 46 | human | 9659 | PDE4DIP | phosphodiesterase 4D intera... | XM_017002888.1 | 34.1% | 29.9% | (many diffs) |
| 47 | human | 9659 | PDE4DIP | phosphodiesterase 4D intera... | XM_017002889.2 | 34.1% | 29.9% | (many diffs) |
| 48 | human | 9659 | PDE4DIP | phosphodiesterase 4D intera... | XM_017002891.2 | 33.1% | 30.2% | (many diffs) |
| 49 | human | 9659 | PDE4DIP | phosphodiesterase 4D intera... | XM_017002892.2 | 33.1% | 30.2% | (many diffs) |
| 50 | human | 9659 | PDE4DIP | phosphodiesterase 4D intera... | XM_017002893.1 | 33.1% | 30.2% | (many diffs) |
| 51 | human | 9659 | PDE4DIP | phosphodiesterase 4D intera... | XM_017002894.2 | 33.1% | 30.2% | (many diffs) |
| 52 | human | 9659 | PDE4DIP | phosphodiesterase 4D intera... | XM_017002895.2 | 33.1% | 30.2% | (many diffs) |
| 53 | mouse | 83679 | Pde4dip | phosphodiesterase 4D intera... | NM_178080.4 | 84.3% | 85.1% | (many diffs) |
| 54 | mouse | 83679 | Pde4dip | phosphodiesterase 4D intera... | NR_110360.1 | 43% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 3462
- ORF length:
- 3396
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgaa ggagatttgc aggatctgtg cccgagagct gtgtggaaac cagcggcgct 121 ggatcttcca cacggcgtcc aagctcaatc tccaggttct gctttcgcac gtcttgggca 181 aggatgtccc ccgcgatggc aaagccgagt tcgcttgcag caagtgtgct ttcatgcttg 241 atcgaatcta tcgattcgac acagttattg cccggattga agcgctttct attgagcgct 301 tgcaaaagct gctactggag aaggatcgcc tcaagttctg cattgccagt atgtatcgga 361 agaataacga tgactctggc gcggagatca aggcggggaa tgggacggtt gacatgtccg 421 tcttacccga tgcgagatac tctgcactgc tccaggagga cttcgcctat tcagggtttg 481 agtgctgggt ggagaatgag gatcagatcc aggagccaca cagctgccat ggttcagaag 541 gccctggaaa ccgacccagg agatgccgtg gttgtgccgc tttgcgggtt gctgattctg 601 actatgaagc catttgtaag gtacctcgaa aggtggccag aagtatctcc tgcggccctt 661 ctagcaggtg gtcgaccagc atttgcactg aagaaccagc gttgtctgag gttgggccac 721 ccgacttagc aagcacaaag gtacccccag atggagaaag catggaggaa gagacgcctg 781 gttcctctgt ggaatctttg gatgcaagcg tccaggctag ccctccacaa cagaaagatg 841 aggagactga gagaagtgca aaggaacttg gaaagtgtga ctgttgttca gatgatcagg 901 ctccgcagca tgggtgtaat cacaagctgg aattagctct tagcatgatt aaaggtcttg 961 attataagcc catccagagc ccccgaggga gcaggcttcc gattccagtg aaatccagcc 1021 tacctggagc caagcctggc cctagcatga cagatggagt tagttccggt ttccttaaca 1081 ggtctttgaa acccctttac aagacacctg tgagttatcc cttggagctt tcagacctgc 1141 aggagctgtg ggatgatctc tgtgaagatt atttgccgct ccgggtccag cccatgactg 1201 aagagttgct gaaacaacaa aagctgaatt cacatgagac cactataact cagcagtctg 1261 tatctgattc ccacttggca gaactccagg aaaaaatcca gcaaacagag gccaccaaca 1321 agattcttca agagaaactt aatgaaatga gctatgaact aaagtgtgct caggagtcgt 1381 ctcaaaagca agatggtaca attcagaacc tcaaggaaac tctgaaaagc agggaacgtg 1441 agactgagga gttgtaccag gtaattgaag gtcaaaatga cacaatggca aagcttcgag 1501 aaatgctgca ccaaagccag cttggacaac ttcacagctc agagggtact tctccagctc 1561 agcaacaggt agctctgctt gatcttcaga gtgctttatt ctgcagccaa cttgaaatac 1621 agaagctcca gagggtggta cgacagaaag agcgccaact ggctgatgcc aaacaatgtg 1681 tgcaatttgt agaggctgca gcacacgaga gtgaacagca gaaagaggct tcttggaaac 1741 ataaccagga attgcgaaaa gccttgcagc agctacaaga agaattgcag aataagagcc 1801 aacagcttcg tgcctgggag gctgaaaaat acaatgagat tcgaacccag gaacaaaaca 1861 tccagcacct aaaccatagt ctgagtcaca aggagcagtt gcttcaggaa tttcgggagc 1921 tcctacagta tcgagataac tcagacaaaa cccttgaagc aaatgaaatg ttgcttgaga 1981 aacttcgcca gcgaatacat gataaagctg ttgctctgga gcgggctata gatgaaaaat 2041 tctctgctct agaagagaaa gaaaaagaac tgcgccagct tcgtcttgct gtgagagagc 2101 gagatcatga cttagagaga ctgcgcgatg tcctctcctc caatgaagct actatgcaaa 2161 gtatggagag tctcctgagg gccaaaggcc tggaagtgga acagttatct actacctgtc 2221 aaaacctcca gtggctgaaa gaagaaatgg aaaccaaatt tagccgttgg cagaaggaac 2281 aagagagtat cattcagcag ttacagacgt ctcttcatga taggaacaaa gaagtggagg 2341 atcttagtgc aacactgctc tgcaaacttg gaccagggca gagtgagata gcagaggagc 2401 tgtgccagcg tctacagcga aaggaaagga tgctgcagga ccttctaagt gatcgaaata 2461 aacaagtgct ggaacatgaa atggagattc aaggcctgct tcagtctgtg agcaccaggg 2521 agcaggaaag ccaagctgct gcagagaagt tggtgcaagc cttaatggaa agaaattcag 2581 aattacaggc cctgcgccaa tatttaggag ggagagactc cctgatgtcc caagcaccca 2641 tctctaacca acaagctgaa gttaccccca ctggccgtct tggaaaacag actgatcaag 2701 gttcaatgca gataccttcc agagatgata gcacttcatt gactgccaaa gaggatgtca 2761 gcatacccag atccacatta ggagacttgg acacagttgc agggctggaa aaagaactga 2821 gtaatgccaa agaggaactt gaactcatgg ctaaaaaaga aagagaaagt cagatggaac 2881 tttctgctct acagtccatg atggctgtgc aggaagaaga gctgcaggtg caggctgctg 2941 atatggagtc tctgaccagg aacatacaga ttaaagaaga tctcataaag gacctgcaaa 3001 tgcaactggt tgatcctgaa gacataccag ctatggaacg cctgacccag gaagtcttac 3061 ttcttcggga aaaagttgct tcagtagaat cccagggtca agaaatttca ggaaaccgaa 3121 gacaacagtt gctgctgatg ctagaaggac TAGTAGATGA ACGGAGTCGG CTCAATGAGG 3181 CCTTACAAGC AGAGAGACAG CTCTATAGCA GTCTGGTGAA GTTCCATGCC CATCCAGAGA 3241 GCTCTGAGAG AGACCGAACT CTGCAGGTGG AACTGGAAGG GGCTCAGGTG TTACGCAGTC 3301 GGCTAGAAGA AGTTCTTGGA AGAAGCTTGG AGCGCTTAAA CAGGCTGGAG ACCCTGGCCG 3361 CCATTGGAGG TGCAGCTGCA GGGGATGACA CCGAAGATAC AAGCACTGAG TTCACTGACA 3421 GTATTGAGGA GGAGGCTGCA CACCATAGTC ACCAGCAACT ATACCCAACT TTCTTGTACA 3481 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 3541 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 3601 AGGACGAATA CATCCAACTT CAGTAGTATC GACGCGTTAA GTCgacaatc aacctctgga 3661 ttacaaaatt tgtgaaagat t