Construct: ORF TRCN0000473676

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF011430.1_s317c1
Derived from:
ccsbBroadEn_06530
DNA Barcode:
TTTATAGTACTATCGAGGGAACTC
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
LMO1 (4004)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000473676
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 4004 LMO1 LIM domain only 1 NM_002315.3 100% 100%
2 human 4004 LMO1 LIM domain only 1 NM_001270428.2 98.9% 98.7% 0_1insATG;7T>C;13C>A
3 human 4004 LMO1 LIM domain only 1 XM_011520098.2 92.9% 92.9% 0_1ins33
4 human 4004 LMO1 LIM domain only 1 XM_011520099.2 92.9% 92.9% 0_1ins33
5 human 4004 LMO1 LIM domain only 1 XM_006718228.3 79.8% 77.7% (many diffs)
6 human 55885 LMO3 LIM domain only 3 NM_001001395.2 74.4% 83.3% (many diffs)
7 human 55885 LMO3 LIM domain only 3 NM_001243609.1 74.4% 83.3% (many diffs)
8 human 55885 LMO3 LIM domain only 3 NM_001243610.1 74.4% 83.3% (many diffs)
9 human 55885 LMO3 LIM domain only 3 NM_018640.5 74.4% 83.3% (many diffs)
10 human 55885 LMO3 LIM domain only 3 XM_006719110.4 74.4% 83.3% (many diffs)
11 human 55885 LMO3 LIM domain only 3 XM_006719111.2 74.4% 83.3% (many diffs)
12 human 55885 LMO3 LIM domain only 3 XM_011520762.1 74.4% 83.3% (many diffs)
13 human 55885 LMO3 LIM domain only 3 XM_011520763.1 74.4% 83.3% (many diffs)
14 human 55885 LMO3 LIM domain only 3 XM_024449058.1 74.4% 83.3% (many diffs)
15 human 4004 LMO1 LIM domain only 1 XM_011520100.1 67.6% 55.4% (many diffs)
16 human 4004 LMO1 LIM domain only 1 NR_073006.2 33% 1_516del;881_1001del;1106_1415del
17 mouse 109594 Lmo1 LIM domain only 1 NM_057173.3 93.1% 98.7% (many diffs)
18 mouse 109594 Lmo1 LIM domain only 1 XM_006507195.3 93.1% 98.7% (many diffs)
19 mouse 109594 Lmo1 LIM domain only 1 NM_001302205.1 91.6% 97.4% (many diffs)
20 mouse 109594 Lmo1 LIM domain only 1 NM_001302206.1 86.3% 91.6% (many diffs)
21 mouse 109593 Lmo3 LIM domain only 3 NM_207222.2 73.1% 83.3% (many diffs)
22 mouse 109593 Lmo3 LIM domain only 3 XM_006505340.1 73.1% 83.3% (many diffs)
23 mouse 109593 Lmo3 LIM domain only 3 XM_006505341.3 73.1% 83.3% (many diffs)
24 mouse 109593 Lmo3 LIM domain only 3 XM_006505342.2 73.1% 83.3% (many diffs)
25 mouse 109593 Lmo3 LIM domain only 3 XM_006505344.3 73.1% 83.3% (many diffs)
26 mouse 109593 Lmo3 LIM domain only 3 XM_011241131.2 73.1% 83.3% (many diffs)
27 mouse 109593 Lmo3 LIM domain only 3 XM_011241132.2 73.1% 83.3% (many diffs)
28 mouse 109593 Lmo3 LIM domain only 3 XM_011241133.2 73.1% 83.3% (many diffs)
29 mouse 109593 Lmo3 LIM domain only 3 XM_017321313.1 73.1% 83.3% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
534
ORF length:
468
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttGGCATGAT GGTGCTGGAC AAGGAGGACG GCGTGCCGAT GCTCTCCGTC CAGCCCAAAG
121GGAAGCAGAA GGGCTGTGCG GGCTGTAACC GCAAGATCAA GGACCGCTAT CTGCTGAAGG
181CATTGGACAA GTACTGGCAC GAAGACTGCC TCAAGTGTGC CTGCTGTGAC TGCCGCCTGG
241GCGAGGTGGG CTCCACCCTC TACACCAAGG CCAACCTCAT CCTGTGCCGA CGCGACTACC
301TGAGGCTCTT TGGCACCACA GGGAACTGTG CTGCTTGCAG CAAGCTGATC CCAGCCTTCG
361AGATGGTGAT GCGGGCCCGG GACAACGTGT ATCACCTCGA CTGCTTCGCC TGCCAGCTCT
421GCAACCAGAG ATTTTGTGTG GGAGACAAAT TCTTCCTGAA GAACAACATG ATCTTGTGTC
481AGATGGACTA TGAGGAAGGG CAGCTCAATG GCACCTTTGA ATCCCAAGTT CAGTACCCAA
541CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG
601ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA
661TATCTTGTGG AAAGGACGAT TTATAGTACT ATCGAGGGAA CTCACGCGTT AAGTCgacaa
721tcaacctctg gattacaaaa tttgtgaaag att

Download FASTA (ORF) (Full)