Transcript: Mouse XM_006507195.3

PREDICTED: Mus musculus LIM domain only 1 (Lmo1), transcript variant X1, mRNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Lmo1 (109594)
Length:
1412
CDS:
640..1110

Additional Resources:

NCBI RefSeq record:
XM_006507195.3
NBCI Gene record:
Lmo1 (109594)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_006507195.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000412467 GTTCAGTAACGCCCACCATCT pLKO_005 1102 CDS 100% 4.050 5.670 N Lmo1 n/a
2 TRCN0000413245 AGCCTGTACAGTCTGTCTTCT pLKO_005 1306 3UTR 100% 4.950 3.960 N Lmo1 n/a
3 TRCN0000417349 AGCCGCTGTCCTACAAGTTAG pLKO_005 1206 3UTR 100% 10.800 7.560 N Lmo1 n/a
4 TRCN0000412264 ATGCTCTCCGTCCAACCTAAG pLKO_005 673 CDS 100% 6.000 4.200 N Lmo1 n/a
5 TRCN0000054564 CATCTCAATGGCACCTTTGAA pLKO.1 1075 CDS 100% 5.625 3.938 N Lmo1 n/a
6 TRCN0000422666 TTTGCGTGGGAGACAAATTCT pLKO_005 1007 CDS 100% 5.625 3.938 N Lmo1 n/a
7 TRCN0000054567 CCTGAAGAACAACATGATCTT pLKO.1 1029 CDS 100% 4.950 3.465 N Lmo1 n/a
8 TRCN0000054566 CCGAGACAATGTGTATCACCT pLKO.1 951 CDS 100% 2.640 1.848 N Lmo1 n/a
9 TRCN0000054565 CGCAGGCTGCAACCGCAAGAT pLKO.1 711 CDS 100% 0.000 0.000 N Lmo1 n/a
10 TRCN0000054563 CGGCGTGACTACCTGAGGCTT pLKO.1 862 CDS 100% 0.000 0.000 N Lmo1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_006507195.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_06530 pDONR223 100% 93.1% 98.7% None (many diffs) n/a
2 ccsbBroad304_06530 pLX_304 0% 93.1% 98.7% V5 (many diffs) n/a
3 TRCN0000473676 TTTATAGTACTATCGAGGGAACTC pLX_317 98.7% 93.1% 98.7% V5 (many diffs) n/a
4 ccsbBroadEn_03676 pDONR223 100% 73.3% 83.3% None (many diffs) n/a
5 ccsbBroad304_03676 pLX_304 0% 73.3% 83.3% V5 (many diffs) n/a
6 TRCN0000467106 AGCGATGATACCCTAACGCCTAGG pLX_317 70.9% 73.3% 83.3% V5 (many diffs) n/a
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