Construct: ORF TRCN0000473861
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002500.1_s317c1
- Derived from:
- ccsbBroadEn_10353
- DNA Barcode:
- CGCCCGCAGATCATTCGATACGCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MT1DP (326343)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473861
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4489 | MT1A | metallothionein 1A | NM_005946.3 | 74.3% | 67.2% | (many diffs) |
| 2 | human | 4495 | MT1G | metallothionein 1G | NM_005950.3 | 73.2% | 67.2% | (many diffs) |
| 3 | human | 4493 | MT1E | metallothionein 1E | NM_175617.4 | 72.6% | 68.8% | (many diffs) |
| 4 | human | 4502 | MT2A | metallothionein 2A | NM_005953.5 | 72.1% | 65.5% | (many diffs) |
| 5 | human | 4501 | MT1X | metallothionein 1X | NM_005952.4 | 72.1% | 62.2% | (many diffs) |
| 6 | human | 4495 | MT1G | metallothionein 1G | NM_001301267.2 | 72% | 66.1% | (many diffs) |
| 7 | human | 645745 | MT1HL1 | metallothionein 1H like 1 | NM_001276687.2 | 71.5% | 62.2% | (many diffs) |
| 8 | human | 4496 | MT1H | metallothionein 1H | NM_005951.2 | 71.5% | 62.2% | (many diffs) |
| 9 | human | 4494 | MT1F | metallothionein 1F | NM_005949.4 | 71% | 63.9% | (many diffs) |
| 10 | human | 4490 | MT1B | metallothionein 1B | NM_005947.3 | 70.4% | 65.5% | (many diffs) |
| 11 | human | 4499 | MT1M | metallothionein 1M | NM_176870.3 | 70.4% | 59% | (many diffs) |
| 12 | human | 326343 | MT1DP | metallothionein 1D, pseudogene | NR_027781.1 | 39% | 1_38del;186_376del | |
| 13 | human | 4498 | MT1JP | metallothionein 1J, pseudogene | NR_036677.1 | 32.3% | (many diffs) | |
| 14 | human | 4500 | MT1L | metallothionein 1L, pseudogene | NR_001447.2 | 30.6% | (many diffs) | |
| 15 | human | 644314 | MT1IP | metallothionein 1I, pseudogene | NR_003669.2 | 28.4% | (many diffs) | |
| 16 | human | 326343 | MT1DP | metallothionein 1D, pseudogene | NR_003658.2 | 27.9% | (many diffs) | |
| 17 | human | 644314 | MT1IP | metallothionein 1I, pseudogene | NR_104045.1 | 25.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 317
- ORF end:
- 464
- ORF length:
- 147
- Sequence:
-
1 GTCGCTTCAT GTGACTCCAC GGAGTACCGG GCGCCGTCCA GGCACCTCGA TTAGTTCTCG 61 AGCTTTTGGA GTACGTCGTC TTTAGGTTGG GGGGAGGGGT TTTATGCGAT GGAGTTTCCC 121 CACACTGAGT GGGTGGAGAC TGAAGTTAGG CCAGCTTGGC ACTTGATGTA ATTCTCCTTG 181 GAATTTGCCC TTTTTGAGTT TGGATCTTGG TTCATTCTCA AGCCTCAGAC AGTGGTTCAA 241 AGTTTTTTTC TTCCATTTCA GGTGTCGTGA GGCTAGCATC GATTGATCAA CAAGTTTGTA 301 CAAAAAAGTT GGCACCATGG ACCTCAGCTG CTCCTGCGCC ACTGGTGGCT CCTGCACCTG 361 TGCCAGCTCC TGCAAATGCA AAGAGTACAA ATGCACCTCC TGCAAGAAGA ACTGCTGCTC 421 CTGCTGCCCC ATGGGCTGTG CCAAATGTGC CCAGGGCTGC ACCTTGCCAA CTTTCTTGTA 481 CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA 541 GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG 601 AAAGGACGAC GCCCGCAGAT CATTCGATAC GCCACGCGTT AAGTCgacaa tcaacctctg 661 gattacaaaa tttgtgaaag att