Construct: ORF TRCN0000474059
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000044.1_s317c1
- Derived from:
- ccsbBroadEn_04460
- DNA Barcode:
- TCGCGCAACGAACCCGAAAAGTAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- FRMD5 (84978)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474059
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 84978 | FRMD5 | FERM domain containing 5 | NM_032892.5 | 100% | 100% | |
| 2 | human | 84978 | FRMD5 | FERM domain containing 5 | NM_001322950.2 | 89.1% | 84.5% | 1476_1477ins179;1524_1525insTGACACC |
| 3 | human | 84978 | FRMD5 | FERM domain containing 5 | XM_005254730.3 | 88.6% | 84.6% | (many diffs) |
| 4 | human | 84978 | FRMD5 | FERM domain containing 5 | NM_001322949.2 | 86.7% | 84.1% | (many diffs) |
| 5 | human | 84978 | FRMD5 | FERM domain containing 5 | NM_001322951.2 | 80.7% | 78.1% | (many diffs) |
| 6 | human | 84978 | FRMD5 | FERM domain containing 5 | NM_001286490.2 | 72.6% | 68.4% | (many diffs) |
| 7 | human | 84978 | FRMD5 | FERM domain containing 5 | NM_001286491.2 | 58.9% | 56.9% | 0_1ins613;25_26ins89 |
| 8 | human | 84978 | FRMD5 | FERM domain containing 5 | XR_931927.3 | 36.4% | 1_201del;1912_4685del | |
| 9 | human | 84978 | FRMD5 | FERM domain containing 5 | NR_104455.2 | 31.8% | (many diffs) | |
| 10 | mouse | 228564 | Frmd5 | FERM domain containing 5 | XM_006499269.3 | 92.6% | 98.4% | (many diffs) |
| 11 | mouse | 228564 | Frmd5 | FERM domain containing 5 | XM_011239466.2 | 88.1% | 93.6% | (many diffs) |
| 12 | mouse | 228564 | Frmd5 | FERM domain containing 5 | XM_017317742.1 | 82.1% | 83.1% | (many diffs) |
| 13 | mouse | 228564 | Frmd5 | FERM domain containing 5 | NM_172673.3 | 81% | 81.9% | (many diffs) |
| 14 | mouse | 228564 | Frmd5 | FERM domain containing 5 | XM_017317740.1 | 80.2% | 82.2% | (many diffs) |
| 15 | mouse | 228564 | Frmd5 | FERM domain containing 5 | XM_017317739.1 | 78.1% | 79.2% | (many diffs) |
| 16 | mouse | 228564 | Frmd5 | FERM domain containing 5 | XM_017317738.1 | 76.4% | 78.7% | (many diffs) |
| 17 | mouse | 228564 | Frmd5 | FERM domain containing 5 | XM_017317734.1 | 76.3% | 78.4% | (many diffs) |
| 18 | mouse | 228564 | Frmd5 | FERM domain containing 5 | XM_017317736.1 | 75.9% | 77.9% | (many diffs) |
| 19 | mouse | 228564 | Frmd5 | FERM domain containing 5 | XM_011239469.2 | 74.1% | 78.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1776
- ORF length:
- 1710
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgct gagcaggttg atgagcggca gcagcaggag cctggagcgc gagtacagct 121 gcaccgtgcg gctgctggac gacagcgagt acacctgcac catccagaga gatgccaaag 181 gccagtacct gtttgacctt ctttgccacc atctgaacct acttgagaaa gactattttg 241 gtatccgctt tgtagaccca gataagcagc ggcattggct ggaatttaca aagtctgtgg 301 tgaaacaatt gagatcccag cctccattca ccatgtgctt ccgtgtgaag ttttatcctg 361 cagaccctgc tgctctgaaa gaagaaataa ccaggtattt agtcttcctg cagatcaaaa 421 gggatctcta ccatggccga ctcctctgta aaacatcgga tgctgccttg ttagcagctt 481 acatccttca agcggagatt ggggattatg actcagggaa acaccctgaa ggctacagct 541 ccaagttcca gtttttccct aaacattcag agaagctgga aaggaaaatt gctgagattc 601 acaagacgga actgagtggt caaacaccag caacatcaga gctgaacttc ttaagaaaag 661 cacagacatt ggaaacatat ggagtggatc ctcacccatg taaggacgtg tcaggaaatg 721 ctgcatttct ggccttcact ccttttgggt ttgttgttct tcaaggaaac aagagggtcc 781 acttcattaa atggaatgag gtgaccaagc tgaaatttga aggaaagact ttctatttat 841 acgtaagtca gaaagaggaa aagaaaatta ttcttacata ttttgctcca actcctgaag 901 cgtgtaagca cctctggaaa tgtggaatcg agaaccaagc cttctacaag ctggagaagt 961 caagccaagt ccgcacagtg tccagcagca atttattctt taaagggagc cggttccgat 1021 acagtggccg agttgcaaag gaagtcatgg aatcaagtgc taagatcaaa cgggagccac 1081 cggaaataca cagagcaggg atggttccca gccggagctg tccctccata acccatggcc 1141 caaggctgag cagcgtcccc aggacccgca gaagagctgt tcacatctcc atcatggaag 1201 gcctagagtc cttacgggac agtgcccatt ccacaccagt gcgttccact tcccatgggg 1261 acaccttcct gcctcacgtg agaagcagcc ggacagatag caatgagcga gtagctgtga 1321 ttgcagacga ggcctacagc cctgcagaca gcgtgctgcc cacccctgtg gctgagcaca 1381 gcctggagct gatgttgctt tcccggcaga tcaatGGAGC CACCTGCAGC ATTGAGGAGG 1441 AGAAGGAATC TGAAGCCAGC ACCCCAACTG CTACAGAGGT GGAGGCCCTT GGGGGAGAGC 1501 TGAGGGCCCT GTGTCAGGGG CACAGCGGGC CCGAGGAGGA ACAGGTGAAT AAGTTTGTTC 1561 TAAGTGTCCT CCGTTTGCTC CTTGTGACCA TGGGACTCCT CTTTGTTTTG CTCCTCCTCC 1621 TGATCATCCT TACCGAGTCT GACCTTGACA TTGCCTTTTT CCGTGATATC CGCCAGACCC 1681 CCGAGTTTGA ACAATTCCAC TATCAATACT TTTGTCCCCT CAGGCGATGG TTTGCCTGCA 1741 AAATCCGCTC AGTGGTGAGC CTGCTCATTG ACACCTGCCC AACTTTCTTG TACAAAGTGG 1801 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 1861 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 1921 ATCGCGCAAC GAACCCGAAA AGTATACGCG TTAAGTCgac aatcaacctc tggattacaa 1981 aatttgtgaa agatt