Construct: ORF TRCN0000474192
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018977.3_s317c1
- Derived from:
- ccsbBroadEn_14764
- DNA Barcode:
- ATTATATGCGTACAATAACACCCC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- PI4KA (5297)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474192
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5297 | PI4KA | phosphatidylinositol 4-kina... | XM_017028830.1 | 57.5% | 15.1% | (many diffs) |
| 2 | human | 728233 | PI4KAP1 | phosphatidylinositol 4-kina... | NR_003563.1 | 52.9% | (many diffs) | |
| 3 | human | 375133 | PI4KAP2 | phosphatidylinositol 4-kina... | NR_003700.1 | 50.4% | (many diffs) | |
| 4 | human | 5297 | PI4KA | phosphatidylinositol 4-kina... | NM_001362862.1 | 40.8% | 10.7% | (many diffs) |
| 5 | human | 5297 | PI4KA | phosphatidylinositol 4-kina... | NM_001362863.1 | 40.6% | 10.7% | (many diffs) |
| 6 | human | 5297 | PI4KA | phosphatidylinositol 4-kina... | NM_058004.4 | 40.2% | 10.6% | (many diffs) |
| 7 | human | 5297 | PI4KA | phosphatidylinositol 4-kina... | XR_937868.1 | 35.8% | (many diffs) | |
| 8 | human | 5297 | PI4KA | phosphatidylinositol 4-kina... | XM_005261635.1 | 26.9% | 10.4% | (many diffs) |
| 9 | mouse | 224020 | Pi4ka | phosphatidylinositol 4-kina... | XM_006522037.2 | 64.6% | 18.6% | (many diffs) |
| 10 | mouse | 224020 | Pi4ka | phosphatidylinositol 4-kina... | NM_001001983.2 | 37% | 10.7% | (many diffs) |
| 11 | mouse | 224020 | Pi4ka | phosphatidylinositol 4-kina... | XM_006522034.1 | 36% | 10.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 777
- ORF length:
- 711
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgcg ggagatggca ggggcctggc acatgacggt ggagcagaaa tttggcctgt 121 tttctgctga gataaaggaa gcagaccccc tggctgcctc ggaagcaagt caacccaaac 181 cctgtccccc cgaagtgacc ccccactaca tctggatcga cttcctggtg cagcggtttg 241 agatcgccaa gtactgcagc tctgaccaag tggagatctt ctccagcctg ctgcagcgct 301 ccatgtccct gaacatcggc ggggccaagg ggagcatgaa ccggcacgtg gcggccatcg 361 ggccccgctt caagctgctg accctggggc tgtccctcct gcatgccgat gtggttccaa 421 atgcaaccat ccgcaatgtg cttcgcgaga agatctactc cactgccttt gactacttca 481 gctgtccccc aaagttccct actcaaggag agaagcggct gcgtgaagac ataagcatca 541 tgattaaatt ttggaccgcc atgttctcag ataagaagta cctgaccgcc agccagcttg 601 ttcccccaga taatcaggac acccggagca acctggacat aactgtcggc tctcggcaac 661 aagccaccca aggctggatc aacacatacc ccctgtccag cggcatgtcc accatctcca 721 agaaatcagc atgtctaaga aaaccaaccg gggctcccag ctgcacaaat actacatgaa 781 gcgcagacgc tgctgctgtc cctgctggcc actgagatcg agcgtctcat cacatggtac 841 acccgctgtc agccccggaa ctggaactag acaaggccga aaaagaacag ccgtggccac 901 tgggagattc taagtacatc agcctgagtg agaagcagtg gaaggacaac gtgaacctcg 961 cctggagcat ctctccctac ctagccgtgc agctgcctgc caggtttaag aacacagaag 1021 ccattgggaa cgaagtgacc cgtctcgttc ggttggaccc gggagccgtt agtgatgtgc 1081 ctgaagcaat caagttcctg gtcacctggc acaccatcga cgccgatgct ccagagctca 1141 gccatgtgct gtgctgggcg cccacggacc cacccacagg cctctcctac ttctccagca 1201 tgtacccgcc gcaccctctc acggcgcagt acggggtgaa agtcctgcgg tccttccctc 1261 cggacgccat cctcttctac atcccccaga ttgtgcaggc cctcaggtac gacaagatgg 1321 gctatgtgcg ggagtatatt ctgtgggcag cgtctaaatc ccagcttctg gcacaccagt 1381 tcatctggaa catgaagact aacatttatc tagatgaaga gggccaccag aaagaccctg 1441 acatcggcga cctcctggat cagttggtag aggagatcac aggctccttg tccggcccag 1501 cgaaggactt ttaccagcgg gagtttgatt tctttaacaa gatcaccaac gtgtcggcta 1561 tcatcaagcc ctaccctaaa ggcgacgaga gaaagaaggc ttgtctgtcg gccctgtctg 1621 aagtgaaggt gcagccgggc tgctacctgc ccagcaaccc tgaggccatt gtgctggaca 1681 tcgactacaa gtctgggacc ccgatgcaga gtgctgcaaa agccccatat ctggccaagt 1741 tcaaggtgaa gcgatgtgga gttagtgaac ttgaaaaaga aggtctgcgg tgccgctcag 1801 actccgagga tgagtcagca agcagaagcc cgacgccaga aagatttctg gcaggcagca 1861 tcttcaaagg tgggagacga ctgccggcaa gacatgctgg ccctgcagat catcgacctc 1921 ntcaaagaac atctcccagg ctgtcggcct ggacctcttt gtttttcccn taccgcgtgg 1981 tggccactgc ccctgggtgc ggggtgatcg agtgcatccc cgactgcacc tcccgggacc 2041 agctgggccg ccagacagac ttcggcatgt acgactactt cacacgccag tacggggatg 2101 agtccactct ggccttccag caggcccgct acaacttcat ccgaagcatg gccgcctaca 2161 gcctcctgct gttcctgctg cagatcaagg acagacacaa cggcaacatt atgctggaca 2221 agaagggtca tatcatccac atcgactttG GCTTCATGTT TGAAAGCTCG CCGGGCGGCA 2281 ATCTCGGCTG GGAACCCGAC ATCAAGCTGA CGGATGAGAT GGTGATGATC ATGGGGGGCA 2341 AGATGGAGGC CACACCCTTC AAGTGGTTCA TGGAGATGTG TGTCCGAGGC TACCTGGCTG 2401 TGCGGCCCTA CATGGACGCG GTCGTCTCCC TGGTCACTCT CATGTTGGAC ACGGGCCTGC 2461 CCTGTTTTCG CGGCCAGACA ATCAAGCTCT TGAAGCACAG GTTTAGCCCC AACATGACTG 2521 AGCGCGAGGC TGCAAATTTC ATCATGAAGG TCATCCAGAG CTGCTTCCTC AGCAACAGGA 2581 GCCGGACCTA CGACATGATC CAGTACTATC AGAATGACAT CCCCTACTTG CCAACTTTCT 2641 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 2701 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 2761 GTGGAAAGGA CGAATTATAT GCGTACAATA ACACCCCACG CGTTAAGTCg acaatcaacc 2821 tctggattac aaaatttgtg aaagatt