Construct: ORF TRCN0000474214
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003496.2_s317c1
- Derived from:
- ccsbBroadEn_12663
- DNA Barcode:
- GCCATTGCAGGTTATACACAAGAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PANK2 (80025)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474214
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 80025 | PANK2 | pantothenate kinase 2 | NM_001324193.2 | 100% | 100% | |
| 2 | human | 80025 | PANK2 | pantothenate kinase 2 | XM_017028077.2 | 100% | 100% | |
| 3 | human | 80025 | PANK2 | pantothenate kinase 2 | XM_017028078.2 | 100% | 100% | |
| 4 | human | 80025 | PANK2 | pantothenate kinase 2 | XM_017028079.2 | 100% | 100% | |
| 5 | human | 80025 | PANK2 | pantothenate kinase 2 | XM_024452002.1 | 100% | 100% | |
| 6 | human | 80025 | PANK2 | pantothenate kinase 2 | NM_001324191.2 | 48% | 48% | 1_435del |
| 7 | human | 80025 | PANK2 | pantothenate kinase 2 | NM_024960.6 | 48% | 48% | 1_435del |
| 8 | human | 80025 | PANK2 | pantothenate kinase 2 | NM_153640.3 | 48% | 48% | 1_435del |
| 9 | human | 80025 | PANK2 | pantothenate kinase 2 | XM_005260836.4 | 48% | 48% | 1_435del |
| 10 | human | 80025 | PANK2 | pantothenate kinase 2 | XM_005260835.3 | 36.7% | 36.7% | 1_693del |
| 11 | human | 80025 | PANK2 | pantothenate kinase 2 | XM_011529364.3 | 24.9% | 21.2% | (many diffs) |
| 12 | human | 80025 | PANK2 | pantothenate kinase 2 | NM_153638.3 | 23.5% | 23.5% | 1_1308del |
| 13 | human | 80025 | PANK2 | pantothenate kinase 2 | NR_136715.2 | 5.1% | 1_879del;1282_7877del | |
| 14 | human | 80025 | PANK2 | pantothenate kinase 2 | XR_002958533.1 | 3.2% | 1_2096del;2499_12294del | |
| 15 | mouse | 74450 | Pank2 | pantothenate kinase 2 | XM_017319300.1 | 90.3% | 98.5% | (many diffs) |
| 16 | mouse | 74450 | Pank2 | pantothenate kinase 2 | XM_017319299.1 | 61.7% | 67.3% | (many diffs) |
| 17 | mouse | 74450 | Pank2 | pantothenate kinase 2 | XM_006500298.3 | 43.4% | 47.3% | (many diffs) |
| 18 | mouse | 74450 | Pank2 | pantothenate kinase 2 | XM_006500299.3 | 43.4% | 47.3% | (many diffs) |
| 19 | mouse | 74450 | Pank2 | pantothenate kinase 2 | XM_017319298.1 | 43.4% | 47.3% | (many diffs) |
| 20 | mouse | 74450 | Pank2 | pantothenate kinase 2 | NM_153501.2 | 27.3% | 29.7% | (many diffs) |
| 21 | mouse | 74450 | Pank2 | pantothenate kinase 2 | XR_001783191.1 | 8.2% | (many diffs) | |
| 22 | mouse | 74450 | Pank2 | pantothenate kinase 2 | XR_001783192.1 | 5.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 90
- ORF end:
- 492
- ORF length:
- 402
- Sequence:
-
1 TCAGACAGTG GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGCT AGCATCGATT 61 GATCAACAAG TTTGTACAAA AAAGTTGGCA TGGCATCTCG TGGAGATAGC ACCAAAGTGG 121 ATAAACTAGT ACGAGATATT TATGGAGGGG ACTATGAGAG GTTTGGACTG CCAGGCTGGG 181 CTGTGGCTTC AAGCTTTGGA AACATGATGA GCAAGGAGAA GCGAGAGGCT GTCAGTAAAG 241 AGGACCTGGC CAGAGCGACT TTGATCACCA TCACCAACAA CATTGGCTCA ATAGCAAGAA 301 TGTGTGCCCT TAATGAAAAC ATTAACCAGG TGGTATTTGT TGGAAATTTC TTGAGAATTA 361 ATACGATCGC CATGCGGCTT TTGGCATATG CTTTGGATTA TTGGTCCAAG GGGCAGTTGA 421 AAGCACTTTT TTCGGAACAC GAGGGTTATT TTGGAGCTGT TGGAGCACTC CTTGAGCTGT 481 TGAAGATCCC GTGCCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 541 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 601 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGAGCC ATTGCAGGTT ATACACAAGA 661 AACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t