Construct: ORF TRCN0000474427
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005097.1_s317c1
- Derived from:
- ccsbBroadEn_03781
- DNA Barcode:
- CCCATCTACTAGCATTGGGAGGTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- C12orf4 (57102)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474427
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 57102 | C12orf4 | chromosome 12 open reading ... | NM_001304811.1 | 100% | 100% | |
| 2 | human | 57102 | C12orf4 | chromosome 12 open reading ... | NM_020374.4 | 100% | 100% | |
| 3 | human | 57102 | C12orf4 | chromosome 12 open reading ... | NM_001346153.1 | 92.2% | 92.2% | 1032_1033ins129 |
| 4 | human | 57102 | C12orf4 | chromosome 12 open reading ... | NM_001346155.1 | 92.2% | 92.2% | 1032_1033ins129 |
| 5 | human | 57102 | C12orf4 | chromosome 12 open reading ... | XM_006718992.3 | 75.6% | 75% | (many diffs) |
| 6 | human | 57102 | C12orf4 | chromosome 12 open reading ... | NM_001346156.1 | 68.6% | 68.6% | 0_1ins519 |
| 7 | human | 57102 | C12orf4 | chromosome 12 open reading ... | NM_001346157.1 | 60.8% | 60.8% | 0_1ins519;513_514ins129 |
| 8 | human | 57102 | C12orf4 | chromosome 12 open reading ... | NM_001352962.1 | 48.5% | 48.5% | 0_1ins852 |
| 9 | human | 57102 | C12orf4 | chromosome 12 open reading ... | NR_144379.1 | 40.1% | 1_125del;848_849ins107;1675_3754del | |
| 10 | human | 57102 | C12orf4 | chromosome 12 open reading ... | NR_144380.1 | 31.6% | 1_125del;852_853ins434;1348_3427del | |
| 11 | human | 57102 | C12orf4 | chromosome 12 open reading ... | NR_144382.1 | 29.4% | (many diffs) | |
| 12 | mouse | 28040 | D6Wsu163e | DNA segment, Chr 6, Wayne S... | NM_138594.3 | 86.2% | 94.2% | (many diffs) |
| 13 | mouse | 28040 | D6Wsu163e | DNA segment, Chr 6, Wayne S... | XM_006506217.1 | 86.2% | 94.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1722
- ORF length:
- 1656
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgaa gaaaaacaga gaaagattct gcaatagaga gagagaattt gtatataaat 121 ttaaagtagg aagtcagtgc ttagaactga gagtgccact caaatttcct gttcaagaga 181 atgccagtca tttgcatgga cgtctgatgc tgctgcacag tttaccgtgc tttatagaaa 241 aagacttaaa agaagctctg actcagttta tagaagaaga atccctcagc gattatgata 301 gagatgctga agcatccctg gcagctgtga aatcaggtga agtagattta catcagctgg 361 cgagtacatg ggccaaagct tatgctgaga ccacgttaga gcatgcaagg cctgaagaac 421 ccagctggga tgaagatttt gcagatgtgt accatgactt aattcattct cctgcctctg 481 aaactctctt aaatttggaa cataattact ttgttagtat ctcagaactg attggtgaaa 541 gagatgtgga gctgaaaaaa ttacgagaga gacaaggtat tgaaatggaa aaagtcatgc 601 aggaattggg aaaatcactg acagatcaag atgtaaattc actggctgct cagcattttg 661 aatcccagca agacctagaa aataaatggt cgaatgaatt aaaacaatca actgccatcc 721 aaaaacaaga gtatcaagaa tgggtaataa aacttcacca agacctaaaa aaccccaaca 781 acagctccct tagtgaggaa attaaagttc agccaagtca gttcagagaa tctgtagaag 841 caattggaag gatttatgag gaacagagaa agttagaaga aagttttacc attcacttag 901 gagcccagtt gaagaccatg cataatttga gattgctgag agcagatatg ctggacttct 961 gtaagcataa aagaaatcat cgaagtggtg tgaaacttca tcggctccaa acagctctgt 1021 cactttattc tacatctctc tgtggcctgg ttttactagt agataatcga attaattcat 1081 atagtggtat taaaagagat tttgccacag tttgccaaga atgcactgac ttccatttcc 1141 cccgaattga agagcaatta gaagttgtcc aacaggtggt actttatgct agaacccagc 1201 gcaggagtaa attgaaagaa tcacttgatt ctggaaacca aaatggagga aatgatgata 1261 agactaagaa tgctgagagg aactatttaa atgttttacc tggggaattt tatattacac 1321 ggcattctaa tctctcagaa atccatgttg ctttccatct ctgtgtggat gaccatgtga 1381 aatcgggaaa catcactgct cgtgatcctG CCATTATGGG ACTCCGAAAT ATACTCAAAG 1441 TTTGCTGTAC CCATGACATC ACAACAATAA GCATTCCTCT CTTGCTGGTA CATGATATGT 1501 CAGAGGAAAT GACTATACCC TGGTGCTTAA GGAGAGCGGA ACTTGTGTTC AAGTGTGTCA 1561 AAGGTTTCAT GATGGAAATG GCTTCATGGG ATGGAGGAAT TTCTAGGACA GTGCAATTTC 1621 TAGTACCACA GAGTATTTCT GAAGAAATGT TTTATCAACT TAGTAACATG CTTCCCCAGA 1681 TCTTCCGAGT ATCATCAACA CTCACTCTGA CATCCAAGCA CTACCCAACT TTCTTGTACA 1741 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 1801 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 1861 AGGACGACCC ATCTACTAGC ATTGGGAGGT TACGCGTTAA GTCgacaatc aacctctgga 1921 ttacaaaatt tgtgaaagat t