Construct: ORF TRCN0000474709
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002449.1_s317c1
- Derived from:
- ccsbBroadEn_12212
- DNA Barcode:
- GAATAAACCAGACAGCCCTTCGGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TBC1D2 (55357)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474709
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55357 | TBC1D2 | TBC1 domain family member 2 | NM_001267572.1 | 99.9% | 100% | 372A>G |
| 2 | human | 55357 | TBC1D2 | TBC1 domain family member 2 | XM_011518843.2 | 99.9% | 100% | 372A>G |
| 3 | human | 55357 | TBC1D2 | TBC1 domain family member 2 | XM_024447606.1 | 99.9% | 100% | 372A>G |
| 4 | human | 55357 | TBC1D2 | TBC1 domain family member 2 | XM_017014899.1 | 96.8% | 96.8% | 1_45del;417A>G |
| 5 | human | 55357 | TBC1D2 | TBC1 domain family member 2 | NM_001267571.2 | 50.3% | 50.4% | 1_1380del;1752A>G |
| 6 | human | 55357 | TBC1D2 | TBC1 domain family member 2 | NM_018421.4 | 49.2% | 49.2% | 1_1380del;1752A>G;2454_2455ins33 |
| 7 | human | 55357 | TBC1D2 | TBC1 domain family member 2 | NR_051978.2 | 44.9% | 1_1346del;1718A>G;2751_3123del | |
| 8 | human | 55357 | TBC1D2 | TBC1 domain family member 2 | XR_929823.2 | 43.4% | (many diffs) | |
| 9 | mouse | 381605 | Tbc1d2 | TBC1 domain family, member 2 | XM_006538049.1 | 84.1% | 86.1% | (many diffs) |
| 10 | mouse | 381605 | Tbc1d2 | TBC1 domain family, member 2 | XM_030253640.1 | 84.1% | 86.1% | (many diffs) |
| 11 | mouse | 381605 | Tbc1d2 | TBC1 domain family, member 2 | XM_006538048.1 | 56% | 57.2% | (many diffs) |
| 12 | mouse | 381605 | Tbc1d2 | TBC1 domain family, member 2 | XM_006538050.2 | 50.5% | 51.6% | (many diffs) |
| 13 | mouse | 381605 | Tbc1d2 | TBC1 domain family, member 2 | NM_198664.3 | 42.6% | 43.6% | (many diffs) |
| 14 | mouse | 381605 | Tbc1d2 | TBC1 domain family, member 2 | XM_006538046.4 | 42.6% | 43.5% | (many diffs) |
| 15 | mouse | 381605 | Tbc1d2 | TBC1 domain family, member 2 | XM_006538047.4 | 38.4% | 37.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1470
- ORF length:
- 1404
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga agcttaccgg acccagaact gcttcctcaa ctccgagatc caccaggtca 121 caaagatctg gagaaaggtg gctgagaagg agaaggccct tctgacgaag tgcgcctacc 181 tccaagccag aaactgccag gtggaaagca agtacctggc cggtctgaga aggctgcagg 241 aggccctggg ggacgaagcc agcgagtgct cagagctgct gaggcagctt gtccaggagg 301 cactgcagtg ggaagctggg gaggcctcat ctgacagcat cgagctgagc cccatcagta 361 agtatgatga gtacggcttc ctgacggtgc ccgactatga ggtggaagac ctgaagctgc 421 tggccaagat ccaggcgttg gagtcacgat cccaccacct gctgggcctc gaggctgtgg 481 atcggccgct gagggagcgc tgggctgccc tgggcgatct tgtgccctca gccgagctca 541 agcagctact gcgggcagga gtaccccgtg aacaccggcc tcgtgtctgg aggtggctgg 601 tccacctccg tgtccagcac ctgcacactc caggctgcta ccaggaactg ctgagccggg 661 gccaggcccg cgagcaccct gctgcccgcc agattgagct ggacctgaac cggaccttcc 721 ccaacaacaa acacttcacc tgccccacct ccagcttccc cgacaagctc cgccgggtgc 781 tgctggcctt ctcctggcag aaccccacca tcggctactg ccagggcctg aacaggctgg 841 cggccattgc cctgctggtc ctagaggagg aggagagcgc cttctggtgc ctGGTGGCCA 901 TTGTGGAGAC CATCATGCCC GCTGATTACT ACTGCAACAC GCTGACGGCA TCCCAGGTGG 961 ACCAGCGGGT GCTCCAGGAC CTGCTCTCGG AGAAGCTGCC CAGGCTGATG GCCCATCTGG 1021 GGCAGCACCA CGTGGATCTC TCCCTCGTCA CCTTCAACTG GTTCCTCGTG GTCTTTGCGG 1081 ACAGTCTCAT TAGCAACATC CTCCTTCGGG TCTGGGATGC CTTCCTGTAC GAGGGGACGA 1141 AGGTGGTGTT TCGCTATGCC TTGGCCATTT TCAAGTACAA CGAGAAGGAG ATCTTGAGGC 1201 TACAGAATGG CCTGGAAATC TACCAGTACC TGCGCTTCTT CACCAAGACC ATCTCCAACA 1261 GCCGGAAGCT GATGAACATC GCCTTCAATG ACATGAACCC CTTCCGCATG AAACAGCTGC 1321 GGCAGCTGCG CATGGTCCAC CGGGAGCGGC TGGAGGCTGA GCTGCGGGAG CTGGAGCAGC 1381 TTAAGGCAGA GTACCTGGAG AGGCGGGCAT CCCGGCGCAG AGCTGTGTCC GAGGGCTGTG 1441 CCAGCGAGGA CGAGGTGGAG GGGGAAGCCT GCCCAACTTC CTTGTACAAA GTGGTTGATA 1501 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 1561 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGAGAATA 1621 AACCAGACAG CCCTTCGGCA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 1681 tgaaagatt