Construct: ORF TRCN0000474728
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005187.1_s317c1
- Derived from:
- ccsbBroadEn_13008
- DNA Barcode:
- TGACCGGACACAAAGGTACTTTTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- FNIP1 (96459)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474728
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 96459 | FNIP1 | folliculin interacting prot... | NM_001346113.2 | 99.9% | 99.8% | 226G>T |
| 2 | human | 96459 | FNIP1 | folliculin interacting prot... | NM_133372.3 | 43.5% | 43.3% | 226G>T;1520_1526delGAGACTT;1532_3498del |
| 3 | human | 96459 | FNIP1 | folliculin interacting prot... | NM_001008738.3 | 41.1% | 40.9% | (many diffs) |
| 4 | human | 96459 | FNIP1 | folliculin interacting prot... | NM_001346114.2 | 39.7% | 39.6% | 218_219ins135;1385_1391delGAGACTT;1397_3363del |
| 5 | mouse | 216742 | Fnip1 | folliculin interacting prot... | NM_173753.4 | 40.2% | 41.8% | (many diffs) |
| 6 | mouse | 216742 | Fnip1 | folliculin interacting prot... | XM_006532847.3 | 40.2% | 41.7% | (many diffs) |
| 7 | mouse | 216742 | Fnip1 | folliculin interacting prot... | XM_006532848.2 | 38.3% | 39.5% | (many diffs) |
| 8 | mouse | 216742 | Fnip1 | folliculin interacting prot... | XM_006532849.3 | 37.9% | 39.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1590
- ORF length:
- 1524
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ccctacgctg ttccagaagc tcttcagcaa gaggaccggg ctgggcgcgc 121 ccggccgcga cgcccgggac ccagattgcg ggttcagttg gcctttacca gagtttgatc 181 caagccagat tcgactgatt gtatatcaag actgtgaaag acgagggaga aatgttttgt 241 ttgactccag tgttaagaga agaaatgagg acatatcagt atcgaaactc tgcagtgatg 301 ctcaagttaa agtctttggg aaatgctgcc aactgaaacc tggaggagac agttcttcct 361 ctttagatag ttctgtgact tcatcttctg atataaaaga ccagtgtctt aagtaccagg 421 gttctcggtg ctcttctgat gccaatatgc ttggagagat gatgtttggc tcagtagcaa 481 tgagctacaa aggatccacc ttaaaaattc atcagattcg ttcccctcca cagctcatgc 541 tcagcaaagt gtttactgct cggactggca gcagtatttg tgggagtctc aatacgctac 601 aagatagtct tgaattcatc aatcaggaca acaatacatt aaaggctgat aataacacag 661 ttattaatgg actgcttgga aatataggtc tttcacagtt ctgcagcccc aggcgggcat 721 tctctgagca gggtccgctc cgcctgatca ggagcgcctc tttctttgca gttcacagca 781 acccaatgga catgcctgga agagagctca atgaggacag agacagtggc atagcacggt 841 ctgcatctct cagcagcttg ctgatcactc catttccttc cccaaactcc tcacttaccc 901 gaagttgtgc cagcagctac cagcgacgtt ggcgacgcag ccaaacaaca agtttggaaa 961 atggggtatt tcctagatgg tctatagaag aaagctttaa tctctcagat gaaagctgtg 1021 gccctaaccc aggaattgtg cggaaaaaga aGATTGCAAT TGGGGTAATC TTTTCATTGT 1081 CCAAAGATGA AGATGAAAAT AACAAATTTA ATGAATTCTT TTTTTCACAT TTTCCTCTCT 1141 TTGAAAGCCA CATGAACAAA TTAAAGAGTG CAATAGAACA GGCTATGAAA ATGAGCCGGA 1201 GATCAGCTGA TGCCAGTCAG AGAAGTTTGG CATATAATCG AATAGTTGAT GCCCTAAATG 1261 AATTCAGAAC AACAATTTGT AATCTTTACA CGATGCCACG AATTGGAGAA CCTGTCTGGC 1321 TTACAATGAT GTCGGGGACT CCAGAAAAGA ACCACCTTTG CTATCGTTTC ATGAAGGAGT 1381 TCACCTTTCT AATGGAAAAT GCTTCCAAAA ATCAATTCTT GCCAGCTCTC ATTACTGCAG 1441 TTCTGACCAA TCATCTTGCC TGGGTTCCAA CAGTCATGCC AAATGGACAA CCACCTATAA 1501 AAATATTTTT AGAAAAGCAT TCCTCTCAGA GTGTGGACAT GTTGGCAAAG ACTCATCCAT 1561 ATAACCCACT TTGGGCACAA CTGGGTATGT ACCCAACTTT CTTGTACAAA GTGGTTGATA 1621 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 1681 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGATGACC 1741 GGACACAAAG GTACTTTTCA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 1801 tgaaagatt