Construct: ORF TRCN0000474736
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013259.1_s317c1
- Derived from:
- ccsbBroadEn_06786
- DNA Barcode:
- GAAGCAAGCTATATACGATGATGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MASP1 (5648)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474736
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5648 | MASP1 | mannan binding lectin serin... | NM_139125.3 | 99.9% | 100% | 1374T>C;1851G>A |
| 2 | human | 5648 | MASP1 | mannan binding lectin serin... | XM_011512989.2 | 98.9% | 98.9% | (many diffs) |
| 3 | human | 5648 | MASP1 | mannan binding lectin serin... | XM_017006869.1 | 96.3% | 96.4% | 0_1ins78;1296T>C;1773G>A |
| 4 | human | 5648 | MASP1 | mannan binding lectin serin... | XM_017006872.1 | 84.3% | 84.4% | 0_1ins339;1035T>C;1512G>A |
| 5 | human | 5648 | MASP1 | mannan binding lectin serin... | NM_001879.5 | 77.8% | 72.1% | (many diffs) |
| 6 | human | 5648 | MASP1 | mannan binding lectin serin... | XM_011512990.2 | 77.1% | 71.3% | (many diffs) |
| 7 | human | 5648 | MASP1 | mannan binding lectin serin... | XM_017006871.1 | 75.7% | 68.6% | (many diffs) |
| 8 | human | 5648 | MASP1 | mannan binding lectin serin... | XM_017006870.2 | 71% | 61.1% | (many diffs) |
| 9 | human | 5648 | MASP1 | mannan binding lectin serin... | NR_033519.2 | 52.9% | (many diffs) | |
| 10 | human | 5648 | MASP1 | mannan binding lectin serin... | NM_001031849.2 | 51.5% | 50.2% | (many diffs) |
| 11 | human | 5648 | MASP1 | mannan binding lectin serin... | XM_011512991.2 | 51% | 49.6% | (many diffs) |
| 12 | human | 5648 | MASP1 | mannan binding lectin serin... | XM_006713701.2 | 48% | 46.7% | (many diffs) |
| 13 | mouse | 17174 | Masp1 | mannan-binding lectin serin... | XM_006521828.3 | 86% | 89.4% | (many diffs) |
| 14 | mouse | 17174 | Masp1 | mannan-binding lectin serin... | XM_006521829.3 | 44.6% | 45.8% | (many diffs) |
| 15 | mouse | 17174 | Masp1 | mannan-binding lectin serin... | XM_006521830.1 | 42.7% | 44.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2253
- ORF length:
- 2184
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gaggtggctg cttctctatt atgctctgtg cttctccctg tcaaaggctt 121 cagcccacac cgtggagcta aacaatatgt ttggccagat ccagtcgcct ggttatccag 181 actcctatcc cagtgattca gaggtgactt ggaatatcac tgtcccagat gggtttcgga 241 tcaagcttta cttcatgcac ttcaacttgg aatcctccta cctttgtgaa tatgactatg 301 tgaaggtaga aactgaggac caggtgctgg caaccttctg tggcagggag accacagaca 361 cagagcagac tcccggccag gaggtggtcc tctcccctgg ctccttcatg tccatcactt 421 tccggtcaga tttctccaat gaggagcgtt tcacaggctt tgatgcccac tacatggctg 481 tggatgtgga cgagtgcaag gagagggagg acgaggagct gtcctgtgac cactactgcc 541 acaactacat tggcggctac tactgctcct gccgcttcgg ctacatcctc cacacagaca 601 acaggacctg ccgagtggag tgcagtgaca acctcttcac tcaaaggact ggggtgatca 661 ccagccctga cttcccaaac ccttacccca agagctctga atgcctgtat accatcgagc 721 tggaggaggg tttcatggtc aacctgcagt ttgaggacat atttgacatt gaggaccatc 781 ctgaggtgcc ctgcccctat gactacatca agatcaaagt tggtccaaaa gttttggggc 841 ctttctgtgg agagaaagcc ccagaaccca tcagcaccca gagccacagt gtcctgatcc 901 tgttccatag tgacaactcg ggagagaacc ggggctggag gctctcatac agggctgcag 961 gaaatgagtg cccagagcta cagcctcctg tccatgggaa aatcgagccc tcccaagcca 1021 agtatttctt caaagaccaa gtgctcgtca gctgtgacac aggctacaaa gtgctgaagg 1081 ataatgtgga gatggacaca ttccagattg agtgtctgaa ggatgggacg tggagtaaca 1141 agattcccac ctgtaaaatt gtagactgta gagccccagg agagctggaa cacgggctga 1201 tcaccttctc tacaaggaac aacctcacca catacaagtc tgagatcaaa tactcctgtc 1261 aggagcccta ttacaagatg ctcaacaata acacaggtat atatacctgt tctgcccaag 1321 gagtctggat gaataaagta ttggggagaa gcctacccac ctgccttcca gagtgtggtc 1381 agccctcccg ctccctgcca agcctggtca agaggatcat tgggggccga aatgctgagc 1441 ccggcctctt cccgtggcag gccctgatag tggtggagga cacttcgaga gtgccaaatg 1501 acaagtggtt tgggagtggg gccctgctct ctgcgtcctg gatcctcaca gcagctcatg 1561 tgctgcgctc ccagcgtaga gacaccacgg tgataccagt ctccaaggag catgtcaccg 1621 tctacctggg cttgcatgat gtgcgagaca aatcgggggc agtcaacagc tcagctgccc 1681 gagtggtgct ccacccagac ttcaacatcc aaaactacaa ccacgatata gctctggtgc 1741 agctgcagga gcctgtgccc ctgggacccc acgttatgcc tgtctgcctg ccaaggcttg 1801 agcctgaagg cccggccccc cacatgctgg gcctggtggc cggctggggc atctccaatc 1861 ccaatgtgac agtggatgag aTCATCAGCA GTGGCACACG GACCTTGTCA GATGTCCTAC 1921 AGTATGTCAA GTTACCCGTG GTGCCTCACG CTGAGTGCAA AACTAGCTAT GAGTCCCGCT 1981 CGGGCAATTA CAGCGTCACG GAGAACATGT TCTGTGCTGG CTACTACGAG GGCGGCAAAG 2041 ACACGTGCCT TGGAGATAGC GGTGGGGCCT TTGTCATCTT TGATGACTTG AGCCAGCGCT 2101 GGGTGGTGCA AGGCCTGGTG TCCTGGGGGG GACCTGAAGA ATGCGGCAGC AAGCAGGTCT 2161 ATGGAGTCTA CACAAAGGTC TCCAATTACG TGGACTGGGT GTGGGAGCAG ATGGGCTTAC 2221 CACAAAGTGT TGTGGAGCCC CAGGTGGAAC GGTTGCCAAC TTTCTTGTAC AAAGTGGTTG 2281 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 2341 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGAGA 2401 AGCAAGCTAT ATACGATGAT GTACGCGTTA AGTCgacaat caacctctgg attacaaaat 2461 ttgtgaaaga tt