Construct: ORF TRCN0000474764
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018078.1_s317c1
- Derived from:
- ccsbBroadEn_06599
- DNA Barcode:
- TTGATAACTTTGGCATCACCATAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MT1H (4496)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474764
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4496 | MT1H | metallothionein 1H | NM_005951.2 | 99.4% | 100% | 66A>G |
| 2 | human | 645745 | MT1HL1 | metallothionein 1H like 1 | NM_001276687.2 | 95% | 95% | (many diffs) |
| 3 | human | 4489 | MT1A | metallothionein 1A | NM_005946.3 | 92.8% | 88.5% | (many diffs) |
| 4 | human | 4495 | MT1G | metallothionein 1G | NM_005950.3 | 92.3% | 90.1% | (many diffs) |
| 5 | human | 4493 | MT1E | metallothionein 1E | NM_175617.4 | 92.3% | 90.1% | (many diffs) |
| 6 | human | 4501 | MT1X | metallothionein 1X | NM_005952.4 | 92.3% | 88.5% | (many diffs) |
| 7 | human | 4502 | MT2A | metallothionein 2A | NM_005953.5 | 91.2% | 90.1% | (many diffs) |
| 8 | human | 4495 | MT1G | metallothionein 1G | NM_001301267.2 | 90.8% | 88.7% | (many diffs) |
| 9 | human | 4494 | MT1F | metallothionein 1F | NM_005949.4 | 90.7% | 86.8% | (many diffs) |
| 10 | human | 4499 | MT1M | metallothionein 1M | NM_176870.3 | 90.1% | 80.3% | (many diffs) |
| 11 | human | 4490 | MT1B | metallothionein 1B | NM_005947.3 | 89% | 86.8% | (many diffs) |
| 12 | human | 4494 | MT1F | metallothionein 1F | NM_001301272.1 | 62.3% | 49.2% | (many diffs) |
| 13 | human | 326343 | MT1DP | metallothionein 1D, pseudogene | NR_027781.1 | 42.8% | (many diffs) | |
| 14 | human | 4498 | MT1JP | metallothionein 1J, pseudogene | NR_036677.1 | 40.9% | (many diffs) | |
| 15 | human | 4500 | MT1L | metallothionein 1L, pseudogene | NR_001447.2 | 38.1% | (many diffs) | |
| 16 | human | 326343 | MT1DP | metallothionein 1D, pseudogene | NR_003658.2 | 31.1% | (many diffs) | |
| 17 | mouse | 17750 | Mt2 | metallothionein 2 | NM_008630.2 | 85.7% | 83.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 314
- ORF end:
- 497
- ORF length:
- 183
- Sequence:
-
1 GTCGCTTCAT GTGACTCCAC GGAGTACCGG GCGCCGTCCA GGCACCTCGA TTAGTTCTCG 61 AGCTTTTGGA GTACGTCGTC TTTAGGTTGG GGGGAGGGGT TTTATGCGAT GGAGTTTCCC 121 CACACTGAGT GGGTGGAGAC TGAAGTTAGG CCAGCTTGGC ACTTGATGTA ATTCTCCTTG 181 GAATTTGCCC TTTTTGAGTT TGGATCTTGG TTCATTCTCA AGCCTCAGAC AGTGGTTCAA 241 AGTTTTTTTC TTCCATTTCA GGTGTCGTGA GGCTAGCATC GATTGATCAA CAAGTTTGTA 301 CAAAAAAGTT GGCATGGACC CCAACTGCTC CTGCGAGGCT GGTGGCTCCT GCGCCTGCGC 361 CGGCTCCTGC AAGTGCAAGA AGTGCAAATG CACCTCCTGC AAGAAGAGCT GCTGCTCCTG 421 TTGCCCCCTG GGCTGTGCCA AGTGTGCCCA GGGCTGCATC TGCAAAGGGG CGTCAGAGAA 481 GTGCAGCTGC TGTGCCTGCC CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT 541 CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG 601 TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GATTGATAAC TTTGGCATCA 661 CCATACACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga aagatt