Transcript: Human NM_001301272.1

Homo sapiens metallothionein 1F (MT1F), transcript variant 2, mRNA.

Source:
NCBI, updated 2019-09-21
Taxon:
Homo sapiens (human)
Gene:
MT1F (4494)
Length:
498
CDS:
117..251

Additional Resources:

NCBI RefSeq record:
NM_001301272.1
NBCI Gene record:
MT1F (4494)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001301272.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000072608 ACAACCTTTCTCCCAGATGTA pLKO.1 352 3UTR 100% 4.950 6.930 N MT1F n/a
2 TRCN0000072612 CGCTGGTTCCTGCAAGTGCAA pLKO.1 161 CDS 100% 0.880 0.616 N MT1F n/a
3 TRCN0000072610 GTAGCAAGTGTGCCCAGGGCT pLKO.1 280 3UTR 100% 0.000 0.000 N MT1F n/a
4 TRCN0000242867 CAAGTGCAAAGAGTGCAAATG pLKO_005 173 CDS 100% 10.800 5.400 Y MT1G n/a
5 TRCN0000072609 GCAAGTGCAAAGAGTGCAAAT pLKO.1 172 CDS 100% 10.800 5.400 Y MT1F n/a
6 TRCN0000153590 GCAAGTGCAAAGAGTGCAAAT pLKO.1 172 CDS 100% 10.800 5.400 Y MT1E n/a
7 TRCN0000072611 CAAATGCACCTCCTGCAAGAA pLKO.1 188 CDS 100% 4.950 2.475 Y MT1F n/a
8 TRCN0000155121 CAAATGCACCTCCTGCAAGAA pLKO.1 188 CDS 100% 4.950 2.475 Y MT1X n/a
9 TRCN0000440406 ATGCACCTCCTGCAAGAAGAG pLKO_005 191 CDS 100% 4.050 2.025 Y MT1A n/a
10 TRCN0000242660 GCAAATGCACCTCCTGCAAGA pLKO_005 187 CDS 100% 4.050 2.025 Y MT1H n/a
11 TRCN0000364944 TGCAAATGCACCTCCTGCAAG pLKO_005 186 CDS 100% 4.050 2.025 Y MT1E n/a
12 TRCN0000156365 CTGCAAGTGCAAAGAGTGCAA pLKO.1 170 CDS 100% 2.640 1.320 Y MT1E n/a
13 TRCN0000155298 GCAAAGAGTGCAAATGCACCT pLKO.1 178 CDS 100% 2.160 1.080 Y MT1X n/a
14 TRCN0000148975 GCAAAGAGTGCAAATGCACTT pLKO.1 178 CDS 100% 0.405 0.203 Y MT2A n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001301272.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_01041 pDONR223 100% 64.8% 55.5% None (many diffs) n/a
2 ccsbBroad304_01041 pLX_304 95.5% 64.8% 55.5% V5 (many diffs) n/a
3 ccsbBroadEn_13207 pDONR223 100% 64.8% 55% None (many diffs) n/a
4 ccsbBroad304_13207 pLX_304 0% 64.8% 55% V5 (many diffs) n/a
5 TRCN0000467056 AGATTTTCATGCAACTTTTCTCGA pLX_317 100% 64.8% 55% V5 (many diffs) n/a
6 ccsbBroadEn_06598 pDONR223 100% 63.4% 49.2% None (many diffs) n/a
7 ccsbBroad304_06598 pLX_304 0% 63.4% 49.2% V5 (many diffs) n/a
8 TRCN0000473594 TGCAAGCTAGAGCAGCCGCGTATG pLX_317 100% 63.4% 49.2% V5 (many diffs) n/a
9 ccsbBroadEn_06599 pDONR223 100% 62.3% 49.2% None (many diffs) n/a
10 ccsbBroad304_06599 pLX_304 0% 62.3% 49.2% V5 (many diffs) n/a
11 TRCN0000474764 TTGATAACTTTGGCATCACCATAC pLX_317 100% 62.3% 49.2% V5 (many diffs) n/a
12 ccsbBroadEn_01042 pDONR223 100% 60.6% 49.2% None (many diffs) n/a
13 ccsbBroad304_01042 pLX_304 0% 60.6% 49.2% V5 (many diffs) n/a
14 TRCN0000470527 TTTTTAACCATTGCTGGCACAAAG pLX_317 100% 60.6% 49.2% V5 (many diffs) n/a
15 ccsbBroadEn_01040 pDONR223 100% 60.6% 52.3% None (many diffs) n/a
16 ccsbBroad304_01040 pLX_304 0% 60.6% 52.3% V5 (many diffs) n/a
17 TRCN0000465872 GTAGAGTCACTGCCTTGTCCTAGG pLX_317 100% 60.6% 52.3% V5 (many diffs) n/a
18 ccsbBroadEn_01045 pDONR223 100% 56.7% 53.9% None (many diffs) n/a
19 ccsbBroad304_01045 pLX_304 0% 56.7% 53.9% V5 (many diffs) n/a
20 TRCN0000472152 TGCCGATATAGCTCTCTATACTCA pLX_317 100% 56.7% 53.9% V5 (many diffs) n/a
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