Construct: ORF TRCN0000474788
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018870.2_s317c1
- Derived from:
- ccsbBroadEn_14798
- DNA Barcode:
- GTGGCTATCTTTATGGTCCATATC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- PRKG2 (5593)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474788
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5593 | PRKG2 | protein kinase cGMP-depende... | NM_001363401.2 | 99.3% | 31.2% | (many diffs) |
| 2 | human | 5593 | PRKG2 | protein kinase cGMP-depende... | NM_006259.2 | 99.3% | 31.2% | (many diffs) |
| 3 | human | 5593 | PRKG2 | protein kinase cGMP-depende... | NM_001282485.2 | 95.5% | 32.4% | (many diffs) |
| 4 | human | 5593 | PRKG2 | protein kinase cGMP-depende... | XM_017008413.1 | 95% | 30.7% | (many diffs) |
| 5 | human | 5593 | PRKG2 | protein kinase cGMP-depende... | XM_017008414.1 | 95% | 30.7% | (many diffs) |
| 6 | human | 5593 | PRKG2 | protein kinase cGMP-depende... | XM_017008415.1 | 95% | 30.7% | (many diffs) |
| 7 | human | 5593 | PRKG2 | protein kinase cGMP-depende... | XM_017008416.1 | 95% | 30.7% | (many diffs) |
| 8 | human | 5593 | PRKG2 | protein kinase cGMP-depende... | XM_017008417.1 | 91.3% | 31.9% | (many diffs) |
| 9 | human | 5593 | PRKG2 | protein kinase cGMP-depende... | XM_017008418.1 | 60.4% | 51.5% | (many diffs) |
| 10 | human | 5593 | PRKG2 | protein kinase cGMP-depende... | NM_001282480.1 | 44.4% | 9.9% | (many diffs) |
| 11 | human | 5593 | PRKG2 | protein kinase cGMP-depende... | NM_001282481.1 | 44.4% | 9.9% | (many diffs) |
| 12 | human | 5593 | PRKG2 | protein kinase cGMP-depende... | NM_001282482.1 | 40.6% | 6.6% | (many diffs) |
| 13 | human | 5593 | PRKG2 | protein kinase cGMP-depende... | NM_001282483.1 | 36.5% | 12.3% | (many diffs) |
| 14 | mouse | 19092 | Prkg2 | protein kinase, cGMP-depend... | NM_008926.4 | 88.2% | 29.5% | (many diffs) |
| 15 | mouse | 19092 | Prkg2 | protein kinase, cGMP-depend... | XM_006534820.3 | 52.9% | 48.7% | (many diffs) |
| 16 | mouse | 19092 | Prkg2 | protein kinase, cGMP-depend... | XM_006534821.2 | 33% | 12.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 804
- ORF length:
- 735
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gggaaatggt tcagtgaaac ctaaacattc taagcaccca gatggacact 121 ctgggaacct caccactgat gctctgcgga acaaggtgac agagctggag agagagttga 181 ggaggaagga tgctgagatc caggagcggg agtaccattt gaaggagctg cgggagcagc 241 tgtcgaagca gactgtggcc attgctgaac tcacagagga gctccagaac aagtgcatcc 301 agctgaacaa gctgcaggat gtggtgcata tgcagggagg aagcccgctt caggcctctc 361 cagataaagt gcctcttgag gtccaccgga agacctctgg attggtctct ctccatagca 421 ggaggggagc aaaggctggc gtgtctgctg agccaacaac ccggacctat gacctgaaca 481 aaccccctga attttccttt gagaaagcaa gagtcagaaa agactccagt gagaagaagc 541 tcattacaga tgcccttaat aaaaatcagt ttctgaaaag actggatcct cagcagatca 601 aagacatggt ggaatgcatg tatgggagaa actatcagca agggagttac attattaagc 661 aaggagaacc aggaaaccat atctttgtgc tggcagaggg tcgactagag gtgttccaag 721 gggagaaatt gctgtcctcc atccctatgt ggaccacatt tggggagctt gccattttat 781 acactgtaca aggactgcct ctgtgaaagc tattaccaat gttaaaacat gggcactaga 841 tcgagaggta ttccagaata taatggagga gggacagtcc aagctagaga tgaacaatac 901 agaaacttcc tcagaagtgt atccttgctg aagaatttac ctgaagataa attaaccaag 961 atcattgact gcttggaagt ggaatactat gacaaaggag attacatcat tagagagggc 1021 gaggaaggaa gtaccttttt cattttggca aaaggaaagg taaaagtaac acagagcaca 1081 gaaggccatg atcaaccaca gctgataaaa acactgcaga aaggagaata ctttggagaa 1141 aaagctctta tcagtgatga tgtcaggtca gctaacatta ttgctgaaga aaatgatgtt 1201 gcatgcctgg ttatagatcg agaaacattc aaccaaactg tcggtacatt tgaagagctg 1261 caaaaatacc ttgaaggata tgtggcaaac ctgaaccgtg atgatgaaaa aagacatgcg 1321 aagcggtcca tgtctaactg gaagctgtcc aaagcactct ctctggaaat gattcagctg 1381 aaggagaagg tggccagatt ttcctcatca tccccattcc agaaccttga gattattgca 1441 acactgggcg ttggtgggtt cggaagagtt gagcttgtta aagtaaaaaa tgagaatgtt 1501 gcttttgcta tgaagtgtat aaggaagaag cacatagttg acaccaagca gcaggagcat 1561 gtctactcag agaagagatt cctagaggag ctgtgctctc catttcattg tgaatttata 1621 tcgtactttc aaggacaata agtatgtata catgcttctg gagcnntgct aggntgggga 1681 gctctggagt atattaaggg acagaggcag ctttgatgaa cccacctcca aattctgcgt 1741 tgcttgtgtg acagaagcat ttgattacct gcatcgacta ggtattatct acagagactt 1801 gaaaccagaa aacttaattc tagatgctga gggttacctt aaattggttg actttggatt 1861 tgcgaagaaa atagggtctg gacagaaaac atggacattc tgtgggactc cagaatatgt 1921 agcTCCTGAA GTCATTCTCA ACAAGGGACA TGACTTCAGT GTGGATTTCT GGTCACTGGG 1981 AATTCTAGTG TATGAGCTCC TAACGGGCAA CCCACCCTTT TCTGGGGTTG ACCAAATGAT 2041 GACCTACAAT TTGATTCTCA AAGGAATTGA AAAAATGGAT TTTCCCAGGA AGATAACACG 2101 ACGACCTGAG GATTTGATTC GGAGGCTTTG CAGGCAAAAT CCAACAGAAA GGCTGGGAAA 2161 TCTGAAGAAT GGAATAAATG ACATTAAGAA ACACAGGTGG TTAAATGGTT TTAATTGGGA 2221 GGGACTGAAA GCACGGAGCC TTCCATCACC TTTGCAAAGA GAGCTCAAGG GACCCATAGA 2281 TCACAGCTAC TTTGACAAAT ATCCTCCTGA AAAGGGAATG CCTCCAGATG AGCTATCAGG 2341 CTGGGATAAA GACTTCTTGC CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT 2401 CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG 2461 TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GAGTGGCTAT CTTTATGGTC 2521 CATATCACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga aagatt