Construct: ORF TRCN0000474851
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017469.1_s317c1
- Derived from:
- ccsbBroadEn_15907
- DNA Barcode:
- AGATCTTTTATGCAACTTCCCCCC
- Epitope Tag:
- V5 (not translated due to frame shift)
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DDX27 (55661)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474851
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55661 | DDX27 | DEAD-box helicase 27 | NM_017895.7 | 96% | 95.9% | 1_93del;1302C>T;2296G>A |
| 2 | human | 55661 | DDX27 | DEAD-box helicase 27 | NM_001348187.1 | 92.4% | 92.3% | (many diffs) |
| 3 | mouse | 228889 | Ddx27 | DEAD (Asp-Glu-Ala-Asp) box ... | NM_153065.3 | 87.5% | 92.4% | (many diffs) |
| 4 | mouse | 228889 | Ddx27 | DEAD (Asp-Glu-Ala-Asp) box ... | XM_006499422.1 | 84.7% | 89.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2361
- ORF length:
- 2295
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgct tgcggacctc ggcttaatcg gaaccatagg cgaggatgac gaggtgccgg 121 tggagcccga gtctgactcc ggggacgagg aagaggaggg gcccattgtg ctgggcagac 181 gacaaaaagc tttggggaag aaccgcagtg ctgatttcaa ccctgatttc gttttcactg 241 agaaggaggg gacgtacgat ggcagctggg ccctggctga tgtcatgagc caactcaaga 301 agaagagggc agccactaca ttagatgaga agattgagaa agttcgaaag aaaaggaaaa 361 cagaggataa agaagccaag tctgggaagt tggaaaagga gaaagaagca aaggaaggct 421 ctgaaccaaa ggagcaggaa gaccttcaag agaatgatga ggaaggctca gaagatgaag 481 cctcggagac tgactactca tcagctgatg agaacatcct caccaaagca gatacactca 541 aagtaaagga tcggaagaag aagaagaaga aaggacagga agcaggagga ttttttgaag 601 atgcatctca gtacgatgaa aacctctcgt tccaggacat gaacctttcc cgccctcttc 661 tgaaggccat tacagccatg ggcttcaagc agcccacccc gatccagaag gcgtgcatac 721 ctgtgggtct attggggaag gacatctgtg cctgtgcagc cactgggaca ggtaaaactg 781 ccgcctttgc cctgcctgtt ttggagcgtc tgatttataa accccgccag gctccagtca 841 cccgcgtgct ggtgctagtg cccacccgag agctgggcat ccaggtgcac tctgtcacca 901 gacagctggc ccagttctgc aacatcacca cctgcctggc tgtgggcggc ttggatgtga 961 agtctcagga agcagctctt cgggcagcgc ctgacatcct catcgccacc ccaggccggc 1021 tcatcgatca cctccacaac tgcccttcct tccacctgag cagcatcgag gtgctcatcc 1081 tggacgaggc tgacaggatg ctggatgagt actttgagga gcagatgaag gagatcatcc 1141 gaatgtgttc ccaccaccgc cagaccatgc tcttctcggc caccatgaca gacgaggtga 1201 aagatctggc ttctgtctcc ttgaagaatc ctgtccggat atttgtgaac agcaacacag 1261 atgtggctcc ctttctgcgg caggagttca tccggatccg gcctaatcgt gaaggagacc 1321 gggaagccat cgtggcagct ttgttgacga ggaccttcac tgaccatgtg atgctgttca 1381 cgcaaaccaa gaagcaggcc caccgcatgc acatcctcct ggggctcatg gggctgcagg 1441 tgggtgagct ccatggcaac ttgtcacaga cgcagcggct ggaggccctc cggcgtttta 1501 aggatgaaca gattgacatc ctcgtggcca ctgatgtggc agcccgtgga cttgacattg 1561 agggggtcaa aacggtaatc aacttcacaa tgcctaatac catcaaacat tatgtccacc 1621 gggtggggcg aacagcacgt gctggcaggg ctgggcgctc agtctctctg gtgggagaag 1681 atgagcggaa gatgctgaag gagattgtaa aagctgccaa ggcccctgtg aaggccagga 1741 tacttcccca agatgtcatc ctcaaattcc gggacaagat tgagaaaatg gagaaagatg 1801 tgtatgcagt tctgcagcta gaggcggagg aaaaagagat gcagcagtca gaagcccaga 1861 tcaatacagc aaagcggctc ctggagaagg ggaaggaggc agtggtccaa gagcccgaga 1921 ggagctggtt ccagaccaaa gaagagagga agaaggagaa aattgccaaa gctctgcagg 1981 aatttgactt ggCCTTAAGA GGAAAGAAGA AAAGGAAGAA GTTTATGAAG GATGCCAAAA 2041 AAAAGGGGGA GATGACAGCA GAGGAAAGGT CTCAGTTTGA AATCCTCAAG GCGCAGATGT 2101 TTGCTGAACG GCTAGCGAAG AGGAATCGCA GAGCCAAGCG GGCCCGAGCA ATGCCCGAGG 2161 AGGAGCCAGT GAGAGGTCCT GCCAAGAAGC AAAAGCAGGG GAAGAAATCT GTATTTGATG 2221 AAGAACTCAC CAACACAAGC AAGAAGGCCC TGAAACAGTA TCGAGCTAGC CCTTCCTTTG 2281 AAGAAAGGAA ACAGTTGGGC TTGCCCCACC AGAGACGAGG AGGAAACTTT AAATCTAAAT 2341 CCAGATACAA GAGGAGGAAG TACCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC 2401 TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA 2461 AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGAAGATC TTTTATGCAA 2521 CTTCCCCCCA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg tgaaagatt