Construct: ORF TRCN0000474996
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016701.1_s317c1
- Derived from:
- ccsbBroadEn_02104
- DNA Barcode:
- TTTTATCCTGCCAGCTCTTGTGTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SLC33A1 (9197)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474996
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9197 | SLC33A1 | solute carrier family 33 me... | NM_001190992.1 | 100% | 100% | |
| 2 | human | 9197 | SLC33A1 | solute carrier family 33 me... | NM_004733.4 | 100% | 100% | |
| 3 | human | 9197 | SLC33A1 | solute carrier family 33 me... | XM_011513311.3 | 81.6% | 81.6% | 961_962ins303 |
| 4 | human | 9197 | SLC33A1 | solute carrier family 33 me... | NM_001363883.1 | 81.4% | 73.9% | 773_774ins188;957_958ins118 |
| 5 | human | 9197 | SLC33A1 | solute carrier family 33 me... | XM_017007463.1 | 58% | 52.6% | 0_1ins625;149_263del |
| 6 | human | 9197 | SLC33A1 | solute carrier family 33 me... | XM_017007464.1 | 50.6% | 42.6% | 0_1ins625;148_149ins188 |
| 7 | human | 9197 | SLC33A1 | solute carrier family 33 me... | XR_001740361.2 | 32.5% | 1_1361del;2506_2507ins118;2891_4583del | |
| 8 | human | 9197 | SLC33A1 | solute carrier family 33 me... | XR_001740362.2 | 31% | 1_1361del;2322_2323ins185;2824_4516del | |
| 9 | human | 9197 | SLC33A1 | solute carrier family 33 me... | XR_002959605.1 | 29.3% | (many diffs) | |
| 10 | mouse | 11416 | Slc33a1 | solute carrier family 33 (a... | NM_001272035.1 | 87.5% | 93.4% | (many diffs) |
| 11 | mouse | 11416 | Slc33a1 | solute carrier family 33 (a... | NM_015728.5 | 87.5% | 93.4% | (many diffs) |
| 12 | mouse | 11416 | Slc33a1 | solute carrier family 33 (a... | NM_001331067.1 | 44.6% | 39.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1713
- ORF length:
- 1647
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc acccaccatc tcccacaagg acagcagccg gcaacggcgg ccagggaatt 121 tcagtcactc tctggatatg aagagcggtc ccctgccgcc aggcggttgg gatgacagtc 181 atttggactc agcgggccgg gaaggggaca gagaagctct tctgggggat accggcactg 241 gcgacttctt aaaagcccca cagagcttcc gggccgaact aagcagcatt ttgctactac 301 tctttcttta cgtgcttcag ggtattcccc tgggcttggc gggaagcatc ccactcattt 361 tgcaaagcaa aaatgttagc tatacagacc aagctttctt cagttttgtc ttttggccct 421 tcagtctcaa attactctgg gccccgttgg ttgatgcggt ctacgttaag aacttcggtc 481 gtcgcaaatc ttggcttgtc ccgacacagt atatactagg actcttcatg atctatttat 541 ccactcaggt ggaccgtttg cttgggaata ccgatgacag aacacccgac gtgattgctc 601 tcactgtggc gttctttttg tttgaattct tggccgccac tcaggacatt gccgtcgatg 661 gttgggcgtt aactatgtta tccagggaaa atgtgggtta tgcttctact tgcaattcgg 721 tgggccaaac agcgggttac tttttgggca atgttttgtt tttggccctt gaatctgccg 781 acttttgtaa caaatatttg cggtttcagc ctcaacccag aggaatcgtt actctttcag 841 atttcctttt tttctgggga actgtatttt taataacaac aacattggtt gcccttctga 901 aaaaagaaaa cgaagtatca gtagtaaaag aagaaacaca agggatcaca gatacttaca 961 agctgctttt tgcaattata aaaatgccag cagttctgac attttgcctt ctgattctaa 1021 ctgcaaagat tggtttttca gcagcagatg ctgtaacagg actgaaattg gtagaagagg 1081 gagtacccaa agaacattta gccttattgg cagttccaat ggttcctttg cagataatac 1141 tgcctctgat tatcagcaaa tacactgcag gtccccagcc attaaacaca ttttacaaag 1201 ccatgcccta cagattattg cttgggttag aatatgccct actggtttgg tggactccta 1261 aagtagaaca tcaaggggga ttccctatat attactatat cgtagtcctg ctgagttatg 1321 ctttacatca ggttacagtg tacagcatgt atgtttctat aatggctttc aatgcaaagg 1381 ttagtgatcc acttattgga ggaacataca tgaccctttt aaataccgtg tccaatctgg 1441 gaggaaactg gccttctaca gtagctcttt ggcttgtaga tcccctcaca gtaaaagagt 1501 gtgtaggagc atcaaaccag aattgtcgaa cacctgatgc tgttgagctt tgcaaaaaac 1561 tgggtggctc atgtgttaca gccctggatg gttattatgt ggagtccatt atttgtgttt 1621 tcattggatt tggttggtgg ttctttcttg gtccaaaatt tAAAAAGTTA CAGGATGAAG 1681 GATCATCTTC GTGGAAATGC AAAAGGAACA ATTACCCAAC TTTCTTGTAC AAAGTGGTTG 1741 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 1801 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGATT 1861 TTATCCTGCC AGCTCTTGTG TTACGCGTTA AGTCgacaat caacctctgg attacaaaat 1921 ttgtgaaaga tt