Construct: ORF TRCN0000475025
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006011.1_s317c1
- Derived from:
- ccsbBroadEn_06297
- DNA Barcode:
- GATTCCCGACGGCGCCTATTGAGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GNB2 (2783)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475025
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2783 | GNB2 | G protein subunit beta 2 | NM_005273.4 | 99.9% | 100% | 954C>T |
| 2 | human | 2782 | GNB1 | G protein subunit beta 1 | NM_001282539.1 | 78.6% | 90.2% | (many diffs) |
| 3 | human | 2782 | GNB1 | G protein subunit beta 1 | NM_002074.5 | 78.6% | 90.2% | (many diffs) |
| 4 | human | 2782 | GNB1 | G protein subunit beta 1 | XM_017001059.2 | 78.6% | 90.2% | (many diffs) |
| 5 | human | 2782 | GNB1 | G protein subunit beta 1 | XM_017001060.2 | 78.6% | 90.2% | (many diffs) |
| 6 | human | 2782 | GNB1 | G protein subunit beta 1 | XM_017001061.2 | 78.6% | 90.2% | (many diffs) |
| 7 | human | 2782 | GNB1 | G protein subunit beta 1 | XM_024446495.1 | 78.6% | 90.2% | (many diffs) |
| 8 | human | 59345 | GNB4 | G protein subunit beta 4 | NM_021629.4 | 71.7% | 90% | (many diffs) |
| 9 | mouse | 14693 | Gnb2 | guanine nucleotide binding ... | NM_010312.4 | 92.7% | 100% | (many diffs) |
| 10 | mouse | 14693 | Gnb2 | guanine nucleotide binding ... | XM_006504553.3 | 92.7% | 100% | (many diffs) |
| 11 | mouse | 14688 | Gnb1 | guanine nucleotide binding ... | XM_017319977.2 | 77.1% | 90.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1086
- ORF length:
- 1020
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgag tgagctggag caactgagac aggaggccga gcagctccgg aaccagatcc 121 gggatgcccg aaaagcatgt ggggactcaa cactgaccca gatcacagct gggctggacc 181 cagtggggag aatccagatg aggacccgga ggaccctccg tgggcacctg gcaaagatct 241 atgccatgca ctgggggacc gactcaaggc tgctggtcag cgcctcccag gatgggaagc 301 tcatcatctg ggacagctac accaccaaca aggtccacgc catcccgctg cgctcctcct 361 gggtaatgac ctgtgcctac gcgccctcag ggaactttgt ggcctgtggg gggttggaca 421 acatctgctc catctacagc ctcaagaccc gcgagggcaa cgtcagggtc agccgggagc 481 tgcctggcca cactgggtac ctgtcgtgtt gccgcttcct ggatgacaac caaatcatca 541 ccagctctgg ggataccacc tgtgcccTGT GGGACATTGA GACAGGCCAG CAGACAGTGG 601 GTTTTGCTGG ACACAGTGGG GATGTGATGT CCCTGTCCCT GGCCCCCGAT GGCCGCACGT 661 TTGTGTCAGG CGCCTGTGAT GCCTCTATCA AGCTGTGGGA CGTGCGGGAT TCCATGTGCC 721 GACAGACCTT CATCGGCCAT GAATCCGACA TCAATGCAGT GGCTTTCTTC CCCAACGGCT 781 ACGCCTTCAC CACCGGCTCT GACGACGCCA CGTGCCGCCT CTTCGACCTG CGGGCCGATC 841 AGGAGCTCCT CATGTACTCC CATGACAACA TCATCTGTGG CATCACCTCT GTTGCCTTCT 901 CGCGCAGCGG ACGGCTGCTG CTCGCTGGCT ACGACGACTT CAACTGCAAC ATCTGGGATG 961 CCATGAAGGG CGACCGTGCA GGAGTCCTCG CTGGCCACGA CAACCGCGTG AGCTGCCTTG 1021 GGGTCACCGA CGATGGCATG GCTGTGGCCA CGGGCTCCTG GGACTCCTTC CTCAAGATCT 1081 GGAACTACCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 1141 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 1201 CTTGGCTTTA TATATCTTGT GGAAAGGACG AGATTCCCGA CGGCGCCTAT TGAGTACGCG 1261 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt