Construct: ORF TRCN0000475189
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016811.1_s317c1
- Derived from:
- ccsbBroadEn_06848
- DNA Barcode:
- CATTTCTGTCCGGTTCTTCAGACT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RBBP5 (5929)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475189
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5929 | RBBP5 | RB binding protein 5, histo... | NM_005057.4 | 99.9% | 100% | 438G>A |
| 2 | human | 5929 | RBBP5 | RB binding protein 5, histo... | XM_011509833.3 | 99.7% | 99.8% | 438G>A;1585_1586insCAG |
| 3 | human | 5929 | RBBP5 | RB binding protein 5, histo... | NM_001193272.2 | 92.8% | 92.9% | 438G>A;1472_1473ins114 |
| 4 | human | 5929 | RBBP5 | RB binding protein 5, histo... | NM_001193273.2 | 76.1% | 76.2% | 0_1ins381;57G>A;1204_1205insCAG |
| 5 | mouse | 213464 | Rbbp5 | retinoblastoma binding prot... | NM_172517.2 | 92.5% | 99% | (many diffs) |
| 6 | mouse | 213464 | Rbbp5 | retinoblastoma binding prot... | XM_006529358.3 | 85.8% | 91.6% | (many diffs) |
| 7 | mouse | 213464 | Rbbp5 | retinoblastoma binding prot... | XM_006529357.2 | 85.6% | 92.5% | (many diffs) |
| 8 | mouse | 213464 | Rbbp5 | retinoblastoma binding prot... | XM_006529359.1 | 66.4% | 72.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1680
- ORF length:
- 1614
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgaa cctcgagttg ctggagtcct ttgggcagaa ctatccagag gaagctgatg 121 gaactttgga ttgtatcagc atggctttga cttgcacctt taacaggtgg ggcacactgc 181 ttgcagttgg ctgtaatgat ggccgaattg tcatctggga tttcttgaca agaggcattg 241 ctaaaataat tagtgcacac atccatccag tgtgttcttt atgctggagc cgagatggtc 301 ataaactcgt gagtgcttcc actgataaca tagtgtcaca gtgggatgtt ctttcaggcg 361 actgtgacca gaggtttcga ttcccttcac ccatcttaaa agtccaatat catccacgag 421 atcagaacaa ggttctcgtg tgtcccatga aatctgctcc tgtcatgttg accctttcag 481 attccaaaca tgttgttctg ccagtggacg atgactccga tttgaacgtt gtggcatctt 541 ttgataggcg aggggaatat atttatacgg gaaacgcaaa aggcaagatt ttggtcctaa 601 aaacagattc tcaggatctt gttgcttcct tcagagtgac aactggaaca agcaatacca 661 cagccattaa gtcaattgag tttgcccgga aggggagttg ctttttaatt aacacggcag 721 atcgaataat cagagtttat gatggcagag aaatcttaac atgtggaaga gatggagagc 781 ctgaacctat gcagaaattg caggatttgg tgaataggac cccatggaag aaatgttgtt 841 tctctgggga tggggaatac atcgtggcag gttctgcccg gcagcatgcc ctgtacatct 901 gggagaagag cattggcaac ctggtgaaga ttctccatgg gacgagagga gaactcctct 961 tggatgtagc ttggcatcct gttcgaccca tcatagcatc catttccagt ggagtggtat 1021 ctatctgggc acagaatcaa gtagaaaact ggagtgcatt tgcaccagac ttcaaagaat 1081 tggatgaaaa tgtagaatac gaagaaaggg aatcagagtt tgatattgaa gatgaagata 1141 agagtgagcc tgagcagaca ggggctgatg ctgcagaaga tgaggaagtg gatgtcacca 1201 gcgtggaccc tattgctgcc ttctgtagca gtgatgaaga gctggaagat tcaaaggctc 1261 tattgtattt acccattgcc cctgaggtag aagacccaga agaaaatcct tacggccccc 1321 caccggatgc agtccaaacc tcctTGATGG ATGAAGGGGC TAGTTCAGAG AAGAAGAGGC 1381 AGTCCTCAGC AGATGGGTCC CAGCCACCTA AGAAGAAACC CAAAACAACC AATATAGAAC 1441 TTCAAGGAGT ACCAAATGAT GAAGTCCATC CACTACTGGG TGTGAAGGGG GATGGCAAAT 1501 CCAAGAAGAA GCAAGCAGGC CGGCCTAAAG GATCAAAAGG TAAAGAGAAA GATTCTCCAT 1561 TTAAACCGAA ACTCTACAAA GGGGACAGAG GTTTACCTCT GGAAGGATCA GCGAAGGGTA 1621 AAGTGCAGGC GGAACTCAGC CAGCCCTTGA CAGCAGGAGG AGCAATCTCA GAACTGTTAT 1681 GCCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 1741 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 1801 TTTATATATC TTGTGGAAAG GACGACATTT CTGTCCGGTT CTTCAGACTA CGCGTTAAGT 1861 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt