Transcript: Human XM_011509833.3

PREDICTED: Homo sapiens RB binding protein 5, histone lysine methyltransferase complex subunit (RBBP5), transcript variant X1, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
RBBP5 (5929)
Length:
4399
CDS:
143..1756

Additional Resources:

NCBI RefSeq record:
XM_011509833.3
NBCI Gene record:
RBBP5 (5929)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_011509833.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000329737 GCTGTAATGATGGCCGAATTG pLKO_005 267 CDS 100% 10.800 15.120 N RBBP5 n/a
2 TRCN0000353567 TGGAGCCGAGATGGTCATAAA pLKO_005 362 CDS 100% 13.200 10.560 N RBBP5 n/a
3 TRCN0000165444 GCCACCTAAGAAGAAACCCAA pLKO.1 1480 CDS 100% 2.640 2.112 N RBBP5 n/a
4 TRCN0000165777 CGTGAGTGCTTCCACTGATAA pLKO.1 385 CDS 100% 13.200 9.240 N RBBP5 n/a
5 TRCN0000329678 TCATTGTACCCAGCGTCATTT pLKO_005 2083 3UTR 100% 13.200 9.240 N RBBP5 n/a
6 TRCN0000369175 TTTCAAGCATAAGTCCAAATA pLKO_005 1948 3UTR 100% 13.200 9.240 N RBBP5 n/a
7 TRCN0000369176 ACCCATCATAGCATCCATTTC pLKO_005 1063 CDS 100% 10.800 7.560 N RBBP5 n/a
8 TRCN0000418649 GTCACAGTGGGATGTTCTTTC pLKO_005 412 CDS 100% 10.800 7.560 N Rbbp5 n/a
9 TRCN0000159189 GCAGATCGAATAATCAGAGTT pLKO.1 794 CDS 100% 4.950 3.465 N RBBP5 n/a
10 TRCN0000329677 GCAGATCGAATAATCAGAGTT pLKO_005 794 CDS 100% 4.950 3.465 N RBBP5 n/a
11 TRCN0000034413 GCCTTCTGTAGCAGTGATGAA pLKO.1 1295 CDS 100% 4.950 3.465 N Rbbp5 n/a
12 TRCN0000034410 GCTCTATTGTATTTACCCATT pLKO.1 1334 CDS 100% 4.050 2.835 N Rbbp5 n/a
13 TRCN0000166086 CCACGAGATCAGAACAAGGTT pLKO.1 491 CDS 100% 3.000 2.100 N RBBP5 n/a
14 TRCN0000165100 CATGGCTTTGACTTGCACCTT pLKO.1 217 CDS 100% 2.640 1.848 N RBBP5 n/a
15 TRCN0000159776 GTAAAGAGAAAGATTCTCCAT pLKO.1 1617 CDS 100% 2.640 1.848 N RBBP5 n/a
16 TRCN0000161384 GCTTCCAGAGATGGAAACAAA pLKO.1 2532 3UTR 100% 0.563 0.394 N RBBP5 n/a
17 TRCN0000431475 AGAGAAAGATTCTCCATTTAA pLKO_005 1621 CDS 100% 15.000 9.000 N Rbbp5 n/a
18 TRCN0000256748 GGCAGGAGAATTGCTTGAATC pLKO_005 4192 3UTR 100% 10.800 5.400 Y SMIM11A n/a
19 TRCN0000222574 CGCCTGTAATCCCAGCACTTT pLKO.1 3067 3UTR 100% 4.950 2.475 Y ERAP2 n/a
20 TRCN0000078113 GCCTGTAATCCCAGCACTTTA pLKO.1 3068 3UTR 100% 13.200 6.600 Y LIAS n/a
21 TRCN0000021429 CACACCTGTAATCCCAGCATT pLKO.1 4024 3UTR 100% 4.950 2.475 Y ERN2 n/a
22 TRCN0000138998 CACACCTGTAATCCCAGCATT pLKO.1 4024 3UTR 100% 4.950 2.475 Y P3H4 n/a
23 TRCN0000344020 CACACCTGTAATCCCAGCATT pLKO_005 4024 3UTR 100% 4.950 2.475 Y P3H4 n/a
24 TRCN0000007228 CACCTGTAATCCCAGCACTTT pLKO.1 4026 3UTR 100% 4.950 2.475 Y CFLAR n/a
25 TRCN0000166635 CACCTGTAATCCCAGCACTTT pLKO.1 4026 3UTR 100% 4.950 2.475 Y C19orf31 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_011509833.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_06848 pDONR223 100% 99.7% 99.8% None 438G>A;1585_1586insCAG n/a
2 TRCN0000475189 CATTTCTGTCCGGTTCTTCAGACT pLX_317 21.1% 99.7% 99.8% V5 438G>A;1585_1586insCAG n/a
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