Construct: ORF TRCN0000475207
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015859.1_s317c1
- Derived from:
- ccsbBroadEn_06761
- DNA Barcode:
- ACGACCATGATACGACGGACCGAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PPP3CC (5533)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475207
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5533 | PPP3CC | protein phosphatase 3 catal... | NM_005605.4 | 99.9% | 100% | 1524G>A |
| 2 | human | 5533 | PPP3CC | protein phosphatase 3 catal... | NM_001243974.1 | 98.2% | 98.2% | 1139_1165del;1551G>A |
| 3 | human | 5533 | PPP3CC | protein phosphatase 3 catal... | NM_001243975.1 | 97.9% | 98% | 1319_1320ins30;1494G>A |
| 4 | human | 5533 | PPP3CC | protein phosphatase 3 catal... | XM_017013611.2 | 79% | 76% | (many diffs) |
| 5 | human | 5533 | PPP3CC | protein phosphatase 3 catal... | XR_001745555.2 | 65.1% | (many diffs) | |
| 6 | human | 5533 | PPP3CC | protein phosphatase 3 catal... | XR_001745556.2 | 56.2% | (many diffs) | |
| 7 | human | 5533 | PPP3CC | protein phosphatase 3 catal... | XR_001745553.2 | 55.9% | (many diffs) | |
| 8 | human | 5533 | PPP3CC | protein phosphatase 3 catal... | XR_001745558.2 | 53.7% | (many diffs) | |
| 9 | human | 5533 | PPP3CC | protein phosphatase 3 catal... | XR_001745554.2 | 51.2% | (many diffs) | |
| 10 | human | 5533 | PPP3CC | protein phosphatase 3 catal... | XR_001745557.2 | 49.3% | (many diffs) | |
| 11 | human | 5533 | PPP3CC | protein phosphatase 3 catal... | XR_001745559.2 | 48.7% | (many diffs) | |
| 12 | mouse | 19057 | Ppp3cc | protein phosphatase 3, cata... | NM_008915.3 | 85.2% | 86.3% | (many diffs) |
| 13 | mouse | 19057 | Ppp3cc | protein phosphatase 3, cata... | NM_001304991.1 | 84.3% | 84.8% | (many diffs) |
| 14 | mouse | 19057 | Ppp3cc | protein phosphatase 3, cata... | XM_006518714.3 | 83.7% | 85.1% | (many diffs) |
| 15 | mouse | 19057 | Ppp3cc | protein phosphatase 3, cata... | XM_006518715.3 | 78.8% | 75.6% | (many diffs) |
| 16 | mouse | 19057 | Ppp3cc | protein phosphatase 3, cata... | XM_017315922.1 | 67.9% | 68.2% | (many diffs) |
| 17 | mouse | 19057 | Ppp3cc | protein phosphatase 3, cata... | NM_001304992.1 | 56.3% | 56.6% | (many diffs) |
| 18 | mouse | 19057 | Ppp3cc | protein phosphatase 3, cata... | XM_017315923.1 | 56.3% | 56.6% | (many diffs) |
| 19 | mouse | 19057 | Ppp3cc | protein phosphatase 3, cata... | XM_017315924.1 | 51.6% | 50% | (many diffs) |
| 20 | mouse | 19057 | Ppp3cc | protein phosphatase 3, cata... | XM_017315925.1 | 51.6% | 50% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1602
- ORF length:
- 1536
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc cgggaggcgc ttccacctct ccaccaccga ccgcgtcatc aaagctgtcc 121 cctttcctcc aacccaacgg cttactttca aggaagtatt tgagaatggg aaacctaaag 181 ttgatgtttt aaaaaaccat ttggtaaagg aaggacgact ggaagaggaa gtagccttaa 241 agataatcaa tgatggggct gccatcctga ggcaagagaa gactatgata gaagtagatg 301 ctccaatcac agtatgtggt gatattcatg gacaattctt tgacctaatg aagttatttg 361 aagttggagg atcacctagt aacacacgct acctctttct gggtgactat gtggacagag 421 gctatttcag tatagagtgt gtgctgtatt tatggagttt aaagattaat catcccaaaa 481 cattgtttct gcttcgggga aatcatgaat gcaggcatct tacagactat ttcaccttca 541 aacaggaatg tcgaatcaaa tattcggaac aggtgtatga tgcctgtatg gagacatttg 601 actgtcttcc tcttgctgcc ctcttaaacc agcagtttct ctgtgtacat ggaggaatgt 661 cacctgaaat tacttcttta gatgacatta ggaaattaga caggtttacg gaacctcccg 721 cctttggacc tgtgtgtgac ctgctttggt ctgatccctc agaggattat ggcaatgaga 781 agaccttgga gcactatacc cacaacactg tccgagggtg ctcttatttc tacagttacc 841 ctgcagtttg tgaatttttg cagaacaata atttactatc aattatcaga gcccatgaag 901 cccaagatgc tgggtatcga atgtacagga agagccaagc cacaggcttt ccatcactta 961 ttacaatttt ctctgccccc aattacctag atgtctataa caataaagct gctgtgttga 1021 aatatgaaaa caatgtcatg aatatcaggc agtttaactg ttctccacac ccctactggc 1081 ttccaaactt tatggatgtt ttcacatggt ctttgccttt tgttggggaa aaagtcacag 1141 agatgctggt aaatgtgctc aacatatgct ctgatgacga actgatttct gatgatgaag 1201 cagaaggaag cactacagtt cgtaaggaga tcatcaggaa taagatcaga gccattggga 1261 agatggcacg ggtcttttca atTCTTCGGC AAGAAAGTGA GAGTGTGCTG ACTCTCAAGG 1321 GCCTGACTCC CACAGGCACA CTCCCTCTGG GCGTCCTCTC AGGAGGCAAG CAGACTATCG 1381 AGACAGCCAC AGTAGAAGCG GTAGAGGCCC GGGAAGCCAT CAGAGGGTTC TCGCTTCAGC 1441 ACAAGATCCG GAGTTTTGAA GAAGCGCGAG GTCTGGACCG AATTAATGAG CGAATGCCAC 1501 CCCGAAAGGA TAGCATACAC GCTGGTGGGC CAATGAAATC TGTAACCTCA GCACACTCAC 1561 ATGCTGCGCA CAGGAGCGAC CAAGGGAAAA AAGCCCATTC ATGCCCAACT TTCTTGTACA 1621 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 1681 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 1741 AGGACGAACG ACCATGATAC GACGGACCGA TACGCGTTAA GTCgacaatc aacctctgga 1801 ttacaaaatt tgtgaaagat t