Construct: ORF TRCN0000475208

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF007266.1_s317c1
Derived from:
ccsbBroadEn_13593
DNA Barcode:
ACTTTGATCTAAAATTGAACCTTC
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
CCDC158 (339965)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000475208
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 339965 CCDC158 coiled-coil domain containi... XR_938730.2 22.9% 1_281del;885delA;958_2944del
2 human 339965 CCDC158 coiled-coil domain containi... XM_011531915.2 21.1% 19% 604delA;677_3198del
3 human 339965 CCDC158 coiled-coil domain containi... XR_938729.2 20.8% 1_280del;884delA;957_3232del
4 human 339965 CCDC158 coiled-coil domain containi... NM_001042784.1 20.2% 18.2% 604delA;677_3339del
5 human 339965 CCDC158 coiled-coil domain containi... XM_005262974.4 20.2% 18.2% 604delA;677_3339del
6 human 339965 CCDC158 coiled-coil domain containi... XM_006714204.3 20.1% 18.2% 604delA;677_3351del
7 human 339965 CCDC158 coiled-coil domain containi... XM_011531908.2 20.1% 18.2% 604delA;677_3351del
8 human 339965 CCDC158 coiled-coil domain containi... XM_011531909.2 20.1% 18.2% 604delA;677_3351del
9 human 339965 CCDC158 coiled-coil domain containi... XM_011531910.3 20.1% 18.2% 604delA;677_3351del
10 human 339965 CCDC158 coiled-coil domain containi... XM_011531911.1 20.1% 18.2% 604delA;677_3351del
11 human 339965 CCDC158 coiled-coil domain containi... XM_011531912.2 20.1% 18.2% 604delA;677_3351del
12 human 339965 CCDC158 coiled-coil domain containi... XM_011531913.1 20.1% 18.2% 604delA;677_3351del
13 human 339965 CCDC158 coiled-coil domain containi... XM_011531914.2 20.1% 18.2% 604delA;677_3351del
14 human 339965 CCDC158 coiled-coil domain containi... XM_017008160.1 20.1% 18.2% 604delA;677_3351del
15 human 339965 CCDC158 coiled-coil domain containi... XM_011531916.2 12.8% 10% (many diffs)
16 human 339965 CCDC158 coiled-coil domain containi... XM_011531917.1 12.8% 10% (many diffs)
17 mouse 320696 Ccdc158 coiled-coil domain containi... XM_006534996.2 19.3% 17.5% (many diffs)
18 mouse 320696 Ccdc158 coiled-coil domain containi... XM_011249479.1 19% 17.2% (many diffs)
19 mouse 320696 Ccdc158 coiled-coil domain containi... XM_006534995.2 18.7% 17% (many diffs)
20 mouse 320696 Ccdc158 coiled-coil domain containi... XM_006534994.2 18.6% 16.9% (many diffs)
21 mouse 320696 Ccdc158 coiled-coil domain containi... XM_006534993.2 18.3% 16.6% (many diffs)
22 mouse 320696 Ccdc158 coiled-coil domain containi... XM_006534992.2 18.1% 16.5% (many diffs)
23 mouse 320696 Ccdc158 coiled-coil domain containi... NM_177230.3 18.1% 16.4% (many diffs)
24 mouse 320696 Ccdc158 coiled-coil domain containi... XM_011249478.1 18% 16.3% (many diffs)
25 mouse 320696 Ccdc158 coiled-coil domain containi... XM_006534991.2 17.9% 16.2% (many diffs)
26 mouse 320696 Ccdc158 coiled-coil domain containi... XM_006534988.2 17.8% 16.2% (many diffs)
27 mouse 320696 Ccdc158 coiled-coil domain containi... XM_006534989.3 17.8% 16.2% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
741
ORF length:
675
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgga atcaaaagct tgggaatcaa ataatgaaga tcttttatca agtagtggtg
121tcacatctaa tggaggttct tcaagttcat tttttgtgtc atctattcgt ggtacaataa
181ttgaaaacac atcttcagct gggactttga cacaggttcc ttttttccct aaatatgaag
241tggaacttga ttctcctaga aaaatcatcc catctcctgg aaaggaacac tttgaacgtg
301ttttggaaga atattcacat caagtcaaag atttacagag aagactaaat gaaagcaatg
361aattgcatga gaaacaaaag ttttattTGA GGCAGTCAGT CATTGATTTG CAAACAAAAC
421TTCAGGAGAT GCAAATGGAG AGAGATGCTA TGGCTGACAT CAGACGAAGG GAGAGTCAGT
481CCCAGGAGGA TTTAAGAAAT CAGCTTCAAA ATACAGTTCA TGAACTTGAA GCTGCCAAAT
541GCCTTAAAGA GGACATGCTG AAAGACAGCA ACACACAGAT AGAGCAACTA CGAAAAATGA
601TGCTTAGTCA TGAGGGAGTG CTTCAAGAAA TCCGGTCAAT CCTAGTTGAC TTTGAAGAAG
661CCTCAGGCAA AAAATATGTG AACATGACAG CATGTCTACT CTGCACTTCC GCAGCTTGGG
721CTCAGCTATT AGTAAAATAC TACCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC
781CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG
841AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGAACTT TGATCTAAAA
901TTGAACCTTC ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt

Download FASTA (ORF) (Full)