Construct: ORF TRCN0000475214
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006759.1_s317c1
- Derived from:
- ccsbBroadEn_01044
- DNA Barcode:
- ATCGACCACCTTCTCGGATCAACG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MT1X (4501)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475214
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4501 | MT1X | metallothionein 1X | NM_005952.4 | 100% | 100% | |
| 2 | human | 4502 | MT2A | metallothionein 2A | NM_005953.5 | 92.9% | 90.1% | (many diffs) |
| 3 | human | 4496 | MT1H | metallothionein 1H | NM_005951.2 | 92.8% | 88.5% | (many diffs) |
| 4 | human | 4489 | MT1A | metallothionein 1A | NM_005946.3 | 91.8% | 85.2% | (many diffs) |
| 5 | human | 4495 | MT1G | metallothionein 1G | NM_005950.3 | 91.2% | 86.8% | (many diffs) |
| 6 | human | 4493 | MT1E | metallothionein 1E | NM_175617.4 | 90.7% | 88.5% | (many diffs) |
| 7 | human | 4495 | MT1G | metallothionein 1G | NM_001301267.2 | 90.3% | 87% | (many diffs) |
| 8 | human | 645745 | MT1HL1 | metallothionein 1H like 1 | NM_001276687.2 | 90.1% | 85.2% | (many diffs) |
| 9 | human | 4499 | MT1M | metallothionein 1M | NM_176870.3 | 90.1% | 81.9% | (many diffs) |
| 10 | human | 4490 | MT1B | metallothionein 1B | NM_005947.3 | 89.6% | 86.8% | (many diffs) |
| 11 | human | 4494 | MT1F | metallothionein 1F | NM_005949.4 | 88.5% | 83.6% | (many diffs) |
| 12 | human | 326343 | MT1DP | metallothionein 1D, pseudogene | NR_027781.1 | 42.8% | (many diffs) | |
| 13 | human | 4498 | MT1JP | metallothionein 1J, pseudogene | NR_036677.1 | 40.6% | (many diffs) | |
| 14 | human | 4500 | MT1L | metallothionein 1L, pseudogene | NR_001447.2 | 37.6% | (many diffs) | |
| 15 | human | 644314 | MT1IP | metallothionein 1I, pseudogene | NR_003669.2 | 36.7% | (many diffs) | |
| 16 | human | 644314 | MT1IP | metallothionein 1I, pseudogene | NR_104045.1 | 33.1% | (many diffs) | |
| 17 | mouse | 17750 | Mt2 | metallothionein 2 | NM_008630.2 | 86.4% | 85.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 211
- ORF end:
- 394
- ORF length:
- 183
- Sequence:
-
1 ATGCGATGGA GTTTCCCCAC ACTGAGTGGG TGGAGACTGA AGTTAGGCCA GCTTGGCACT 61 TGATGTAATT CTCCTTGGAA TTTGCCCTTT TTGAGTTTGG ATCTTGGTTC ATTCTCAAGC 121 CTCAGACAGT GGTTCAAAGT TTTTTTCTTC CATTTCAGGT GTCGTGAGGC TAGCATCGAT 181 TGATCAACAA GTTTGTACAA AAAAGTTGGC ATGGACCCCA ACTGCTCCTG CTCGCCTGTT 241 GGCTCCTGTG CCTGTGCCGG CTCCTGCAAA TGCAAAGAGT GCAAATGCAC CTCCTGCAAG 301 AAGAGCTGCT GCTCCTGCTG CCCTGTGGGC TGTGCCAAGT GTGCCCAGGG CTGCATCTGC 361 AAAGGGACGT CAGACAAGTG CAGCTGCTGT GCCTGCCCAA CTTTCTTGTA CAAAGTGGTT 421 GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT 481 AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAA 541 TCGACCACCT TCTCGGATCA ACGACGCGTT AAGTCgacaa tcaacctctg gattacaaaa 601 tttgtgaaag att