Construct: ORF TRCN0000475275

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF007037.1_s317c1
Derived from:
ccsbBroadEn_08410
DNA Barcode:
GAATCTTAACCAGAACTAATTATC
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
DYM (54808)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000475275
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 54808 DYM dymeclin NM_017653.6 99.9% 99.8% 1222G>T
2 human 54808 DYM dymeclin NM_001353211.3 99.8% 99.7% 138_139insAGT;1219G>T
3 human 54808 DYM dymeclin NM_001353210.3 99.8% 99.5% 763_764insCAG;1219G>T
4 human 54808 DYM dymeclin NM_001374433.1 95% 93% (many diffs)
5 human 54808 DYM dymeclin XM_006722488.3 95% 93% (many diffs)
6 human 54808 DYM dymeclin NM_001374434.1 94.2% 94.1% 1222G>T;1745_1746ins114
7 human 54808 DYM dymeclin NM_001374435.1 94.1% 93.8% 763_764insCAG;1219G>T;1742_1743ins114
8 human 54808 DYM dymeclin XM_011526042.2 94% 90% (many diffs)
9 human 54808 DYM dymeclin NM_001374436.1 93.6% 93.4% 494_495ins126;1096G>T
10 human 54808 DYM dymeclin NM_001353214.3 92.3% 92.2% 1222G>T;1458_1622del
11 human 54808 DYM dymeclin NM_001374428.1 92.3% 92.2% 1222G>T;1458_1622del
12 human 54808 DYM dymeclin NM_001353212.3 92.2% 92.1% 138_139insAGT;1219G>T;1455_1619del
13 human 54808 DYM dymeclin NM_001353213.3 92.2% 91.9% 763_764insCAG;1219G>T;1455_1619del
14 human 54808 DYM dymeclin NM_001374429.1 92% 91.8% (many diffs)
15 human 54808 DYM dymeclin XM_011526039.2 91.9% 88.5% (many diffs)
16 human 54808 DYM dymeclin NM_001353216.3 90.8% 90.7% 1222G>T;1563_1564ins183
17 human 54808 DYM dymeclin NM_001374437.1 90.8% 90.5% 763_764ins183;1039G>T
18 human 54808 DYM dymeclin NM_001374438.1 90.6% 90.4% 763_764insCAG;1219G>T;1560_1561ins183
19 human 54808 DYM dymeclin XM_017025800.2 90.6% 90.4% 763_764insCAG;1219G>T;1560_1561ins183
20 human 54808 DYM dymeclin NM_001374439.1 90.5% 90.2% (many diffs)
21 human 54808 DYM dymeclin XM_017025801.1 90.5% 90.2% (many diffs)
22 human 54808 DYM dymeclin XM_011526036.2 89.7% 81.5% 1222G>T;1859_1976del;2126_2235del
23 human 54808 DYM dymeclin XM_011526037.1 89.6% 81.3% (many diffs)
24 human 54808 DYM dymeclin XM_011526038.2 89.6% 81.2% (many diffs)
25 human 54808 DYM dymeclin XM_017025795.1 89.4% 81.1% (many diffs)
26 human 54808 DYM dymeclin NM_001374440.1 88.5% 88.3% 193_194ins228;994G>T
27 human 54808 DYM dymeclin NM_001374430.1 87.9% 85.9% (many diffs)
28 human 54808 DYM dymeclin NM_001374431.1 87.1% 87% 1222G>T;1458_1622del;1910_1911ins114
29 human 54808 DYM dymeclin NM_001374432.1 86.5% 86.3% 494_495ins126;1096G>T;1332_1496del
30 human 54808 DYM dymeclin NM_001353215.3 83.9% 83.8% 1222G>T;1458_1622del;1728_1729ins183
31 human 54808 DYM dymeclin XM_011526040.1 81.8% 73.3% (many diffs)
32 human 54808 DYM dymeclin XM_011526041.2 81.5% 73.5% (many diffs)
33 human 54808 DYM dymeclin XM_017025796.2 81.4% 73.5% (many diffs)
34 human 54808 DYM dymeclin XM_006722492.4 74.5% 72.9% (many diffs)
35 human 54808 DYM dymeclin XR_002958177.1 73.6% (many diffs)
36 human 54808 DYM dymeclin NM_001374442.1 71.5% 71.3% 193_194ins570;652G>T
37 human 54808 DYM dymeclin NM_001374443.1 71.4% 71.3% 191_192ins573;649G>T
38 human 54808 DYM dymeclin NM_001374441.1 66.1% 65.8% 193_194ins570;652G>T;888_1052del
39 human 54808 DYM dymeclin NM_001374444.1 62.4% 62.1% 193_194ins570;652G>T;993_994ins183
40 mouse 69190 Dym dymeclin NM_027727.2 86.1% 94.6% (many diffs)
41 mouse 69190 Dym dymeclin XM_006526216.3 77.9% 85.6% (many diffs)
42 mouse 69190 Dym dymeclin XM_006526217.3 68% 68.3% (many diffs)
43 mouse 69190 Dym dymeclin XM_006526218.3 64.6% 68.3% (many diffs)
44 mouse 69190 Dym dymeclin XR_386014.3 64.4% (many diffs)
45 mouse 69190 Dym dymeclin XM_006526219.3 63.8% 70.7% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
2073
ORF length:
2007
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatggg atcgaatagc agcagaatcg gcgatcttcc taaaaatgag tacttgaaaa
121agttatcagg cacggaatct atctctgaga atgacccgtt ctggaatcag cttctctcat
181tttctttccc tgcaccaact agcagtagtg agttgaaact cttggaggaa gcaaccattt
241cagtctgcag gtcattagtt gaaaacaatc ctcgaacagg aaatcttggt gcactaatta
301aggtcttcct ttctagaacc aaagaactaa aactttcagc agaatgtcag aaccacatct
361tcatttggca gacacacaat gctttgttta ttatttgctg tttgctgaaa gtgttcatct
421gtcagatgtc agaggaggaa ttacaacttc attttactta tgaagaaaaa tctcctggca
481attacagttc tgactcagaa gatcttttgg aagaattgct gtgctgtttg atgcagttga
541tcactgatat tccactctta gatattacat atgaaatatc agtagaagct atatcaacaa
601tggttgtttt cctttcctgc caactcttcc acaaagaagt tttgcgacag agcatcagcc
661acaagtattt gatgcgaggt ccatgtcttc catacaccag caaacttgtg aagaccttat
721tatataactt tatcagacaa gaaaagccac ctcctccagg ggcccatgtt ttccctcagc
781agtcggatgg gggaggactg ctttatggac ttgcatcagg agtagcaaca ggactctgga
841ctgtcttcac actaggtggt gtgggcagca aagcggctgc ctctccagag ctttcttccc
901ctctggccaa ccagagtctc ctgcttctgc tggtgttggc caatctgaca gatgcctcag
961atgcgccaaa cccctacaga caagccatta tgtccttcaa gaacacacaa gatagcagtc
1021ctttcccctc atcaattcca catgccttcc agatcaactt taatagtttg tacacagctc
1081tttgtgaaca gcagacatct gatcaagcaa ctctcctctt gtataccttg ctccatcaaa
1141atagtaatat tagaacatac atgttggctc gcacagatat ggaaaatctt gttttaccaa
1201ttcttgagat tctgtatcat gttgaagaaa ggaattcaca ccatgtgtat atggccctta
1261taatattgtt gatccttacg gaagattatg gcttcaacag atccattcat gaagtgatac
1321taaaaaatat tacttggtat tcagaacgag ttttaactga aatctccttg gggagtctcc
1381tgatcctggt ggtaataaga accattcaat acaacatgac taggacacga gacaagtacc
1441ttcacacaaa ttgtttggca gctttagcaa atatgtcggc acagtttcgt tctctccatc
1501agtatgctgc ccagaggatc atcagtttat tttctttgct gtctaaaaaa cacaacaaag
1561ttctggaaca agccacacag tccttgagag gttcgctgag ttctaatgat gttccTCTAC
1621CAGATTATGC ACAAGACCTA AATGTCATTG AAGAAGTGAT TCGAATGATG TTAGAGATCA
1681TCAACTCCTG CCTGACAAAT TCCCTTCACC ACAACCCAAA CTTGGTATAC GCCCTGCTTT
1741ACAAACGCGA TCTCTTTGAA CAATTTCGAA CTCATCCTTC ATTTCAGGAT ATAATGCAAA
1801ATATTGATCT GGTGATCTCC TTCTTTAGCT CAAGGTTGCT GCAAGCTGGA GCTGAGCTGT
1861CAGTGGAACG GGTCCTGGAA ATCATTAAGC AAGGCGTCGT TGCGCTGCCC AAAGACAGAC
1921TGAAGAAATT TCCAGAATTG AAATTCAAAT ATGTGGAAGA GGAGCAGCCC GAGGAGTTTT
1981TTATCCCCTA TGTCTGGTCT CTTGTCTACA ACTCAGCAGT CGGCCTGTAC TGGAATCCAC
2041AGGACATCCA GCTGTTCACC ATGGATTCCG ACTGCCCAAC TTTCTTGTAC AAAGTGGTTG
2101ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA
2161GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGAGA
2221ATCTTAACCA GAACTAATTA TCACGCGTTA AGTCgacaat caacctctgg attacaaaat
2281ttgtgaaaga tt

Download FASTA (ORF) (Full)