Construct: ORF TRCN0000475283
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007688.1_s317c1
- Derived from:
- ccsbBroadEn_10518
- DNA Barcode:
- TATGCATTGACCTGAGATATCGAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TMEM185B (79134)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475283
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 79134 | TMEM185B | transmembrane protein 185B | NM_024121.3 | 99.9% | 100% | 111A>G |
| 2 | human | 84548 | TMEM185A | transmembrane protein 185A | NM_032508.4 | 80.4% | 88.5% | (many diffs) |
| 3 | human | 84548 | TMEM185A | transmembrane protein 185A | NM_001174092.2 | 67.5% | 73.7% | (many diffs) |
| 4 | human | 84548 | TMEM185A | transmembrane protein 185A | NM_001282302.2 | 39.3% | 44.7% | (many diffs) |
| 5 | mouse | 226351 | Tmem185b | transmembrane protein 185B | NM_146103.2 | 88.8% | 96% | (many diffs) |
| 6 | mouse | 236848 | Tmem185a | transmembrane protein 185A | XM_006527981.2 | 80.9% | 88.8% | (many diffs) |
| 7 | mouse | 236848 | Tmem185a | transmembrane protein 185A | NM_001033328.2 | 74.3% | 81.4% | (many diffs) |
| 8 | mouse | 236848 | Tmem185a | transmembrane protein 185A | XM_017318471.1 | 62.2% | 68% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1116
- ORF length:
- 1050
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgaa ccccaggggc ctgttccagg acttcaaccc cagtaagttt ctcatctaca 121 cctgcctgct gctcttctcg gtgctgctgc ccctccgcct ggacggcatc atccagtgga 181 gctactgggc cgtctttgcc cccatatggc tgtggaagct tctagtcgtc gcaggcgcct 241 ccgtgggcgc gggcgtttgg gcccgcaacc ctcgctaccg caccgaggga gaggcctgtg 301 tggagttcaa agccatgctg atcgctgtgg gcatccacct gctgctgctc atgttcgaag 361 tcctggtctg cgacagggtg gagaggggca cccacttctg gctgctggtc ttcatgcctc 421 tcttcttcgt gtcccccgtg tccgtggctg cctgcgtctg gggctttcga cacgataggt 481 cgctggagct ggagatcctg tgctcggtca acatcctgca gttcatcttc atcgccctaa 541 agctggacag gattattcac tggccgtggc tggtggtgtt tgtgcccctg tggatcctca 601 tgtcgttcct ttgcctggtc gtcctctatt acatcgtctg gtccctcctg ttcctgcggt 661 ccctGGATGT GGTTGCCGAG CAGCGGAGAA CACACGTGAC CATGGCTATC AGTTGGATAA 721 CGATTGTCGT GCCTCTGCTC ACTTTTGAGG TCCTGCTGGT TCACAGATTG GATGGCCACA 781 ATACATTCTC CTACGTCTCC ATATTTGTCC CCCTTTGGCT TTCCTTACTA ACTTTAATGG 841 CCACAACATT TAGGCGAAAG GGGGGCAATC ATTGGTGGTT TGGCATTCGC AGAGACTTCT 901 GTCAGTTTCT GCTTGAAATT TTCCCATTTT TAAGAGAATA TGGGAACATT TCATATGATC 961 TCCATCACGA AGATAGTGAA GATGCTGAAG AAACATCAGT TCCAGAAGCT CCGAAAATTG 1021 CTCCAATATT TGGAAAGAAG GCCAGAGTAG TTATAACCCA GAGCCCTGGG AAATACGTTC 1081 CCCCCCCTCC CAAGTTAAAT ATTGATATGC CAGATTACCC AACTTTCTTG TACAAAGTGG 1141 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 1201 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 1261 ATATGCATTG ACCTGAGATA TCGATACGCG TTAAGTCgac aatcaacctc tggattacaa 1321 aatttgtgaa agatt