Construct: ORF TRCN0000475326
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011060.1_s317c1
- Derived from:
- ccsbBroadEn_02211
- DNA Barcode:
- AACGAAGATCTATTACCCAAATTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GDA (9615)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475326
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9615 | GDA | guanine deaminase | NM_004293.5 | 100% | 100% | |
| 2 | human | 9615 | GDA | guanine deaminase | NM_001242505.2 | 96.3% | 96.3% | 1363_1413del |
| 3 | human | 9615 | GDA | guanine deaminase | NM_001351572.2 | 96.3% | 96.3% | 1363_1413del |
| 4 | human | 9615 | GDA | guanine deaminase | XM_005252317.2 | 96.3% | 96.3% | 1363_1413del |
| 5 | human | 9615 | GDA | guanine deaminase | NM_001351573.2 | 94% | 93.7% | (many diffs) |
| 6 | human | 9615 | GDA | guanine deaminase | XM_024447720.1 | 94% | 93.7% | (many diffs) |
| 7 | human | 9615 | GDA | guanine deaminase | XM_011519217.2 | 92.2% | 92.2% | 384_497del |
| 8 | human | 9615 | GDA | guanine deaminase | NM_001351571.2 | 90.6% | 90.5% | 1_1delAins127 |
| 9 | human | 9615 | GDA | guanine deaminase | XM_011519213.2 | 89.1% | 89.1% | 384_497del;1477_1527del |
| 10 | human | 9615 | GDA | guanine deaminase | XM_011519214.2 | 89.1% | 89.1% | 384_497del;1477_1527del |
| 11 | human | 9615 | GDA | guanine deaminase | XM_011519222.2 | 89.1% | 89.1% | 384_497del;1477_1527del |
| 12 | human | 9615 | GDA | guanine deaminase | XM_017015333.2 | 89.1% | 89.1% | 384_497del;1477_1527del |
| 13 | human | 9615 | GDA | guanine deaminase | XM_011519220.1 | 87.4% | 87.2% | 1_1delAins127;1237_1287del |
| 14 | human | 9615 | GDA | guanine deaminase | XM_011519215.2 | 86.9% | 86.6% | (many diffs) |
| 15 | human | 9615 | GDA | guanine deaminase | XM_011519216.2 | 86.9% | 86.6% | (many diffs) |
| 16 | human | 9615 | GDA | guanine deaminase | XM_017015335.2 | 86.9% | 86.6% | (many diffs) |
| 17 | human | 9615 | GDA | guanine deaminase | NM_001242506.3 | 83.7% | 83.7% | 0_1ins222 |
| 18 | human | 9615 | GDA | guanine deaminase | NM_001242507.2 | 83.7% | 83.7% | 0_1ins222 |
| 19 | human | 9615 | GDA | guanine deaminase | XM_011519218.2 | 81.2% | 81.1% | (many diffs) |
| 20 | human | 9615 | GDA | guanine deaminase | XM_011519219.1 | 80.8% | 80.7% | 1_1delAins127;258_371del;1351_1401del |
| 21 | human | 9615 | GDA | guanine deaminase | XM_011519221.1 | 80.6% | 80.6% | 0_1ins222;1141_1191del |
| 22 | human | 9615 | GDA | guanine deaminase | XM_017015338.1 | 76.6% | 74.8% | (many diffs) |
| 23 | human | 9615 | GDA | guanine deaminase | XR_929878.2 | 74.5% | 1_198del;582_695del;1675_1827del | |
| 24 | human | 9615 | GDA | guanine deaminase | NR_147240.2 | 69.3% | 1_120del;1483_1964del | |
| 25 | human | 9615 | GDA | guanine deaminase | XM_011519223.2 | 60.2% | 60.2% | 0_1ins510;853_903del |
| 26 | human | 9615 | GDA | guanine deaminase | XM_017015340.2 | 60.2% | 60.2% | 0_1ins510;853_903del |
| 27 | human | 9615 | GDA | guanine deaminase | XR_002956833.1 | 43.4% | (many diffs) | |
| 28 | human | 9615 | GDA | guanine deaminase | XR_001746423.2 | 43.2% | 1_198del;582_695del;1675_3150del | |
| 29 | human | 9615 | GDA | guanine deaminase | XR_001746424.2 | 30.2% | 1_198del;582_695del;1675_4499del | |
| 30 | human | 9615 | GDA | guanine deaminase | XR_002956834.1 | 25.4% | (many diffs) | |
| 31 | mouse | 14544 | Gda | guanine deaminase | NM_010266.2 | 88.2% | 90.7% | (many diffs) |
| 32 | mouse | 14544 | Gda | guanine deaminase | XM_006526680.1 | 79.5% | 82.1% | (many diffs) |
| 33 | mouse | 14544 | Gda | guanine deaminase | XM_017318064.1 | 73.3% | 75.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1428
- ORF length:
- 1362
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtg tgccgctcag atgccgcccc tggcgcacat cttccgaggg acgttcgtcc 121 actccacctg gacctgcccc atggaggtgc tgcgggatca cctcctcggc gtgagcgaca 181 gcggcaaaat agtgttttta gaagaagcat ctcaacagga aaaactggcc aaagaatggt 241 gcttcaagcc gtgtgaaata agagaactga gccaccatga gttcttcatg cctgggctgg 301 ttgatacaca catccatgcc tctcagtatt cctttgctgg aagtagcata gacctgccac 361 tcttggagtg gctgaccaag tacacatttc ctgcagaaca cagattccag aacatcgact 421 ttgcagaaga agtatatacc agagttgtca ggagaacact aaagaatgga acaaccacag 481 cttgttactt tgcaacaatt cacactgact catctctgct ccttgccgac attacagata 541 aatttggaca gcgggcattt gtgggcaaag tttgcatgga tttgaatgac acttttccag 601 aatacaagga gaccactgag gaatcgatca aggaaactga gagatttgtg tcagaaatgc 661 tccaaaagaa ctattctaga gtgaagccca tagtgacacc acgtttttcc ctctcctgct 721 ctgagacttt gatgggtgaa ctgggcaaca ttgctaaaac ccgtgatttg cacattcaga 781 gccatataag tgaaaatcgt gatgaagttg aagctgtgaa aaacttatac cccagttata 841 aaaactacac atctgtgtat gataaaaaca atcttttgac aaataagaca gtgatggcac 901 acggctgcta cctctctgca gaagaactga acgtattcca tgaacgagga gcatccatcg 961 cacactgtcc caattctaat ttatcgctca gcagtggatt tctaaatgtg ctagaagtcc 1021 TGAAACATGA AGTCAAGATA GGGCTGGGTA CAGACGTGGC TGGTGGCTAT TCATATTCCA 1081 TGCTTGATGC AATCAGAAGA GCAGTGATGG TTTCCAATAT CCTTTTAATT AATAAGGTAA 1141 ATGAGAAAAG CCTCACCCTC AAAGAAGTCT TCAGACTAGC TACTCTTGGA GGAAGCCAAG 1201 CCCTGGGGCT GGATGGTGAG ATTGGAAACT TTGAAGTGGG CAAGGAATTT GATGCCATCC 1261 TGATCAACCC CAAAGCATCC GACTCTCCCA TTGACCTGTT TTATGGGGAC TTTTTTGGTG 1321 ATATTTCTGA GGCTGTTATC CAGAAGTTCC TCTATCTAGG AGATGATCGA AATATTGAAG 1381 AGGTTTATGT GGGCGGAAAG CAGGTGGTTC CGTTTTCCAG CTCAGTGTAC CCAACTTTCT 1441 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 1501 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 1561 GTGGAAAGGA CGAAACGAAG ATCTATTACC CAAATTCACG CGTTAAGTCg acaatcaacc 1621 tctggattac aaaatttgtg aaagatt