Construct: ORF TRCN0000475390
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014245.1_s317c1
- Derived from:
- ccsbBroadEn_12247
- DNA Barcode:
- TTACACTGAACGGGCTATGATCAG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NBPF1 (55672)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475390
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 284565 | NBPF15 | NBPF member 15 | NM_001170755.2 | 87.5% | 85.1% | (many diffs) |
| 2 | human | 284565 | NBPF15 | NBPF member 15 | NM_173638.4 | 87.5% | 85.1% | (many diffs) |
| 3 | human | 84224 | NBPF3 | NBPF member 3 | NM_001256417.3 | 69.9% | 62.1% | (many diffs) |
| 4 | human | 84224 | NBPF3 | NBPF member 3 | NM_001330381.2 | 68.5% | 60.8% | (many diffs) |
| 5 | human | 84224 | NBPF3 | NBPF member 3 | XM_006710958.1 | 68.5% | 60.8% | (many diffs) |
| 6 | human | 84224 | NBPF3 | NBPF member 3 | XM_006710959.2 | 68.5% | 60.8% | (many diffs) |
| 7 | human | 84224 | NBPF3 | NBPF member 3 | XM_011542276.1 | 68.5% | 60.8% | (many diffs) |
| 8 | human | 84224 | NBPF3 | NBPF member 3 | XM_011542277.1 | 68.5% | 60.8% | (many diffs) |
| 9 | human | 84224 | NBPF3 | NBPF member 3 | XM_011542278.1 | 68.5% | 60.8% | (many diffs) |
| 10 | human | 84224 | NBPF3 | NBPF member 3 | XM_011542281.1 | 68.5% | 60.8% | (many diffs) |
| 11 | human | 84224 | NBPF3 | NBPF member 3 | XM_017002499.2 | 68.5% | 60.8% | (many diffs) |
| 12 | human | 84224 | NBPF3 | NBPF member 3 | XM_024450188.1 | 68.5% | 60.8% | (many diffs) |
| 13 | human | 84224 | NBPF3 | NBPF member 3 | XM_024450190.1 | 68.5% | 60.8% | (many diffs) |
| 14 | human | 84224 | NBPF3 | NBPF member 3 | XM_017002500.1 | 63.8% | 57.2% | (many diffs) |
| 15 | human | 84224 | NBPF3 | NBPF member 3 | XM_017002501.1 | 63.8% | 57.2% | (many diffs) |
| 16 | human | 84224 | NBPF3 | NBPF member 3 | XM_017002502.1 | 63.8% | 57.2% | (many diffs) |
| 17 | human | 102724250 | LOC102724250 | neuroblastoma breakpoint fa... | XM_024452498.1 | 63.3% | 62.8% | (many diffs) |
| 18 | human | 84224 | NBPF3 | NBPF member 3 | NM_032264.5 | 63.2% | 56.1% | (many diffs) |
| 19 | human | 728841 | NBPF8 | NBPF member 8 | NM_001037501.3 | 62.3% | 60.9% | (many diffs) |
| 20 | human | 400818 | NBPF9 | NBPF member 9 | NM_001037675.3 | 62.3% | 60.6% | (many diffs) |
| 21 | human | 84224 | NBPF3 | NBPF member 3 | XM_006710957.1 | 58.9% | 52.8% | (many diffs) |
| 22 | human | 84224 | NBPF3 | NBPF member 3 | NM_001256416.3 | 56.9% | 51.1% | (many diffs) |
| 23 | human | 200030 | NBPF11 | NBPF member 11 | NM_001101663.4 | 54.6% | 46.5% | (many diffs) |
| 24 | human | 400818 | NBPF9 | NBPF member 9 | NM_001277444.1 | 53% | 51.7% | (many diffs) |
| 25 | human | 200030 | NBPF11 | NBPF member 11 | NM_183372.6 | 51.7% | 44% | (many diffs) |
| 26 | human | 149013 | NBPF12 | NBPF member 12 | XM_024453443.1 | 49.6% | 48.2% | (many diffs) |
| 27 | human | 55672 | NBPF1 | NBPF member 1 | NM_017940.6 | 49.5% | 49.4% | (many diffs) |
| 28 | human | 102724250 | LOC102724250 | neuroblastoma breakpoint fa... | XM_017030107.2 | 47.5% | 21.2% | (many diffs) |
| 29 | human | 101929780 | NBPF25P | NBPF member 25, pseudogene | NR_104217.1 | 46.3% | (many diffs) | |
| 30 | human | 101060684 | NBPF26 | NBPF member 26 | NM_001351372.1 | 44.1% | 43% | (many diffs) |
| 31 | human | 200030 | NBPF11 | NBPF member 11 | XM_024453858.1 | 43% | 40% | (many diffs) |
| 32 | human | 149013 | NBPF12 | NBPF member 12 | NM_001278141.1 | 40.3% | 39.2% | (many diffs) |
| 33 | human | 200030 | NBPF11 | NBPF member 11 | NR_110864.2 | 31.7% | (many diffs) | |
| 34 | human | 200030 | NBPF11 | NBPF member 11 | XR_002959662.1 | 31.6% | (many diffs) | |
| 35 | human | 200030 | NBPF11 | NBPF member 11 | XR_002959669.1 | 31.4% | (many diffs) | |
| 36 | human | 200030 | NBPF11 | NBPF member 11 | XR_002959670.1 | 29.9% | (many diffs) | |
| 37 | human | 200030 | NBPF11 | NBPF member 11 | NR_046188.4 | 29.5% | (many diffs) | |
| 38 | human | 84224 | NBPF3 | NBPF member 3 | NR_046176.3 | 29.4% | (many diffs) | |
| 39 | human | 343505 | NBPF7 | NBPF member 7 | XM_017001186.1 | 28.1% | 22% | (many diffs) |
| 40 | human | 200030 | NBPF11 | NBPF member 11 | XM_024453859.1 | 25.5% | 23.6% | (many diffs) |
| 41 | human | 728841 | NBPF8 | NBPF member 8 | NR_102405.2 | 22% | (many diffs) | |
| 42 | human | 728841 | NBPF8 | NBPF member 8 | NR_102404.2 | 21.3% | (many diffs) | |
| 43 | human | 25832 | NBPF14 | NBPF member 14 | NM_015383.2 | 20.3% | 19% | (many diffs) |
| 44 | human | 100132406 | NBPF10 | NBPF member 10 | NM_001039703.6 | 15.8% | 14.8% | (many diffs) |
| 45 | human | 100132406 | NBPF10 | NBPF member 10 | NM_001302371.3 | 15.1% | 14.1% | (many diffs) |
| 46 | human | 101060226 | NBPF19 | NBPF member 19 | NM_001351365.1 | 14.9% | 13.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1875
- ORF length:
- 1809
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgct gaggaatgag cgacagttca aggaggagaa gcttgcagag cagctcaagc 121 aagctgagga gctcaggcaa tataaagtcc tggttcactc tcaggaacga gagctgaccc 181 agttaaggga gaagttacgg gaagggagag atgcctcctg ctcattgaat cagcatctcc 241 aggccctcct cactccggat gagccagaca agtcccaggg gcaggacctc caagaacagc 301 tggctgaggg gtgtagactg gcacagcacc ttgtccaaaa gctcagccca gaaaatgaca 361 acgatgacga tgaagatgtt caagttgagg tggctgagaa agtgcagaaa tcgtctgccc 421 ccagggagat gccgaaggct gaagaaaagg aagtccctga ggactcactg gaggaatgtg 481 ccatcacttg ttcaaatagc catggccctt atgactccaa ccagccacat aggaaaacca 541 aaatcacatt tgaggaagac aaagtcgact caactctcat tggctcatcc tctcatgttg 601 aatgggagga tgctgtacac attatcccag aaaatgaaag tgatgatgag gaagaggaag 661 aaaaagggcc agtgtctccc aggaatctgc aggagtctga agaggaggaa gtcccccagg 721 agtcctggga tgaaggttat tcgactctct caattcctcc tgaaatgttg gcctcgtaca 781 agtcttacag cggcacattt cactcattag aggaacagca agtctgcatg gctgttgaca 841 taggcggaca tcggtgggat caagtgaaaa aggaggacca agaggcaaca ggtcccaggc 901 tcagcaggga gctgctggat gagaaagggc ctgaagtctt gcaggactca ctggatagat 961 gttattcaac tccttcaggt tatcttgaac tgactgactc atgccagccc tacagaagtg 1021 ccttttacat attggagcaa cagcgtgttg gctgggctct tgacatggat gaaattgaaa 1081 agtaccaaga agtggaagaa gaccaagacc catcatgccc caggctcagc agggagctgc 1141 tggatgagaa agagcctgaa gtcttgcagg actcactgga tagatgttat tcgactcctt 1201 caggttatct tgaactgcct gacttaggcc agccctacag aagtgctgtt tactcattgg 1261 aggaacagta ccttggcttg gctcttgacg tggacagaat taaaaaggac caagaagagg 1321 aagaagacca aggcccacca tgccccaggc tcagcaggga gctgctggag gcagtagagc 1381 ctgaagtctt gcaggactca ctggatagat gttattcaac tccttccagt tgtcttgaac 1441 agcctgactc ctgcctgccc tatggaagtt ccttttatgc attggaggaa aaacatgttg 1501 gcttttctct tgacgtggga gaaattGAAA AGAAGGGGAA GGGGAAGAAA AGAAGGGGAA 1561 GAAGATCAAC GAAGAAAAGA AGGAGAAGGG GAAGAAAAGA AGGGGAAGAA GATCAAAACC 1621 CACCATGCCC CAGGCTCAGC GGTGTGCTGA TGGAAGTGGA AGAGCCTGAA GTCTTACAGG 1681 ACTCACTGGA TAGATGTTAT TCGACTCCGT CAATGTTCTT TGAACTACCT GACTCATTCC 1741 AGCACTACAG AAGTGTGTTT TACTCATTTG AGGAACAGCA CATCAGCTTC GCCCTTGACG 1801 TGGACAATAG GTTTCTTACT TTGATGGGAA GAAGTCTCCA CCTGGTCTTC CAGATGGGAG 1861 TCATATTCCC ACAATACCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 1921 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 1981 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA TTACACTGAA CGGGCTATGA 2041 TCAGACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt