Transcript: Human NM_001101663.4

Homo sapiens NBPF member 11 (NBPF11), transcript variant 1, mRNA.

Source:
NCBI, updated 2019-08-05
Taxon:
Homo sapiens (human)
Gene:
NBPF11 (200030)
Length:
4789
CDS:
326..2923

Additional Resources:

NCBI RefSeq record:
NM_001101663.4
NBCI Gene record:
NBPF11 (200030)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001101663.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000282776 TGGATGTCAGGATGCTCTAAA pLKO_005 1072 CDS 100% 13.200 7.920 N NBPF11 n/a
2 TRCN0000352961 CTGAGGAGCTCAGGCAATATA pLKO_005 591 CDS 100% 15.000 7.500 Y NBPF11 n/a
3 TRCN0000244546 CTTGACGTGGACAGAATTAAA pLKO_005 2564 CDS 100% 15.000 7.500 Y NBPF11 n/a
4 TRCN0000255811 GAGGAGCTCAGGCAATATAAA pLKO_005 593 CDS 100% 15.000 7.500 Y NBPF15 n/a
5 TRCN0000369471 TCTTGACGTGGACAGAATTAA pLKO_005 2563 CDS 100% 15.000 7.500 Y NBPF14 n/a
6 TRCN0000242329 TGAGGAGCTCAGGCAATATAA pLKO_005 592 CDS 100% 15.000 7.500 Y NBPF9 n/a
7 TRCN0000244558 AGAGTGCAAAGACCTCATAAA pLKO_005 1321 CDS 100% 13.200 6.600 Y NBPF10 n/a
8 TRCN0000242430 AGTCCTGGGATGAAGGTTATT pLKO_005 2001 CDS 100% 13.200 6.600 Y NBPF11 n/a
9 TRCN0000369532 CACCAACTGCTCTTGACAATT pLKO_005 3641 3UTR 100% 13.200 6.600 Y NBPF14 n/a
10 TRCN0000161314 GACTCACTGGATAGATGTTAT pLKO.1 2225 CDS 100% 13.200 6.600 Y NBPF1 n/a
11 TRCN0000167775 GCAGCAGAACAAATACAAATA pLKO.1 1297 CDS 100% 13.200 6.600 Y NBPF11 n/a
12 TRCN0000344135 GCATGTCTCTGAGCTTCTATA pLKO_005 3458 3UTR 100% 13.200 6.600 Y NBPF15 n/a
13 TRCN0000161173 GCTGTTGACATGGATGAAATT pLKO.1 2336 CDS 100% 13.200 6.600 Y NBPF14 n/a
14 TRCN0000352962 GGAGTGATCAGTCGGACATTT pLKO_005 3378 3UTR 100% 13.200 6.600 Y NBPF11 n/a
15 TRCN0000282774 GTCCTGGGATGAAGGTTATTC pLKO_005 2002 CDS 100% 13.200 6.600 Y NBPF11 n/a
16 TRCN0000161288 GTGCCATCACTTGTTCAAATA pLKO.1 945 CDS 100% 13.200 6.600 Y NBPF1 n/a
17 TRCN0000242432 TGTGCCATCACTTGTTCAAAT pLKO_005 944 CDS 100% 13.200 6.600 Y NBPF11 n/a
18 TRCN0000244547 TTCCAGATGGGAGTCATATTC pLKO_005 3121 3UTR 100% 13.200 6.600 Y NBPF11 n/a
19 TRCN0000242328 TTCTAGAAATCAACGAGAAAT pLKO_005 375 CDS 100% 13.200 6.600 Y NBPF9 n/a
20 TRCN0000242325 ACGAGAGCTGACCCAGTTAAG pLKO_005 634 CDS 100% 10.800 5.400 Y NBPF9 n/a
21 TRCN0000242327 ATTCGACTCCTTCAGGTTATC pLKO_005 2469 CDS 100% 10.800 5.400 Y NBPF9 n/a
22 TRCN0000364788 CAATAAGCAGCCCTTACTAAG pLKO_005 3145 3UTR 100% 10.800 5.400 Y NBPF14 n/a
23 TRCN0000242431 GTATTGGAGCAACAGCGTATT pLKO_005 2309 CDS 100% 10.800 5.400 Y NBPF11 n/a
24 TRCN0000263707 TATTGGAGCAACAGCGTATTG pLKO_005 2310 CDS 100% 10.800 5.400 Y NBPF11 n/a
25 TRCN0000164466 CAGCAGCACATTTCACTCATT pLKO.1 2068 CDS 100% 4.950 2.475 Y NBPF14 n/a
26 TRCN0000146474 CCTGAGTTTCATAGGAGGTAA pLKO.1 3903 3UTR 100% 4.950 2.475 Y NBPF15 n/a
27 TRCN0000163889 CCTGAGTTTCATAGGAGGTAA pLKO.1 3903 3UTR 100% 4.950 2.475 Y NBPF14 n/a
28 TRCN0000161501 GACGATGAAGATGTTCAAGTT pLKO.1 1646 CDS 100% 4.950 2.475 Y NBPF14 n/a
29 TRCN0000128566 GATGACGATGAAGATGTTCAA pLKO.1 1643 CDS 100% 4.950 2.475 Y NBPF15 n/a
30 TRCN0000127801 GCAGAGACAATGCTGTGAGTT pLKO.1 4249 3UTR 100% 4.950 2.475 Y NBPF15 n/a
31 TRCN0000165817 GCTGTTGACATAGGCAGACAT pLKO.1 2111 CDS 100% 4.950 2.475 Y NBPF14 n/a
32 TRCN0000165321 GTCTTCCAGATGGGAGTCATA pLKO.1 3118 3UTR 100% 4.950 2.475 Y NBPF1 n/a
33 TRCN0000181020 CTGGATGAGAAAGAGCCTGAA pLKO.1 2195 CDS 100% 4.050 2.025 Y NBPF8 n/a
34 TRCN0000163782 GCTGTTTACTCATTGGAGGAA pLKO.1 2525 CDS 100% 2.640 1.320 Y NBPF1 n/a
35 TRCN0000244560 GAGGATGCTGTACACATTATT pLKO_005 1886 CDS 100% 15.000 7.500 Y NBPF10 n/a
36 TRCN0000364789 AGGATGCTGTACACATTATTC pLKO_005 1887 CDS 100% 13.200 6.600 Y NBPF14 n/a
37 TRCN0000172811 GCAGGACTCACTGGATAGATT pLKO.1 2221 CDS 100% 5.625 2.813 Y NBPF3 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001101663.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_15343 pDONR223 79.7% 86.2% 77.7% None (many diffs) n/a
2 ccsbBroad304_15343 pLX_304 0% 86.2% 77.7% V5 (many diffs) n/a
3 TRCN0000476676 CTCGGTCCAAATACGACCGTGACC pLX_317 14.4% 86.2% 77.7% V5 (many diffs) n/a
4 TRCN0000469491 AAATCTAACGTTAGACAGGGAAAT pLX_317 16.1% 72% 72% V5 (not translated due to frame shift) (many diffs) n/a
5 ccsbBroadEn_15282 pDONR223 73.8% 68.9% 67% None (many diffs) n/a
6 ccsbBroad304_15282 pLX_304 0% 68.9% 67% V5 (many diffs) n/a
7 ccsbBroadEn_09966 pDONR223 100% 61.5% 53% None (many diffs) n/a
8 ccsbBroad304_09966 pLX_304 0% 61.5% 53% V5 (many diffs) n/a
9 TRCN0000476622 TTTTACCGTGCTGGCCAAGGACTT pLX_317 19.5% 61.5% 53% V5 (many diffs) n/a
10 ccsbBroadEn_12247 pDONR223 100% 54.6% 46.5% None (many diffs) n/a
11 ccsbBroad304_12247 pLX_304 0% 54.6% 46.5% V5 (many diffs) n/a
12 TRCN0000475390 TTACACTGAACGGGCTATGATCAG pLX_317 19.6% 54.6% 46.5% V5 (many diffs) n/a
13 ccsbBroadEn_12795 pDONR223 100% 33.7% 22.7% None (many diffs) n/a
14 ccsbBroad304_12795 pLX_304 0% 33.7% 22.7% V5 (many diffs) n/a
15 TRCN0000466247 ATCAGCAGGTCACACTGTTGAGTG pLX_317 41.2% 33.7% 22.7% V5 (many diffs) n/a
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