Construct: ORF TRCN0000475651
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003283.1_s317c1
- Derived from:
- ccsbBroadEn_08424
- DNA Barcode:
- CCTCGCCGCGTCTCCTGACATAAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SNRK (54861)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475651
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 54861 | SNRK | SNF related kinase | NM_001100594.1 | 99.7% | 99.6% | (many diffs) |
| 2 | human | 54861 | SNRK | SNF related kinase | NM_017719.5 | 99.7% | 99.6% | (many diffs) |
| 3 | human | 54861 | SNRK | SNF related kinase | XM_005265245.3 | 99.7% | 99.6% | (many diffs) |
| 4 | human | 54861 | SNRK | SNF related kinase | NM_001330750.1 | 72.8% | 72.6% | (many diffs) |
| 5 | mouse | 20623 | Snrk | SNF related kinase | NM_001164572.1 | 82.1% | 88.8% | (many diffs) |
| 6 | mouse | 20623 | Snrk | SNF related kinase | NM_133741.2 | 82.1% | 88.8% | (many diffs) |
| 7 | mouse | 20623 | Snrk | SNF related kinase | XM_006511998.3 | 82.1% | 88.8% | (many diffs) |
| 8 | mouse | 20623 | Snrk | SNF related kinase | XM_006511999.3 | 82.1% | 88.8% | (many diffs) |
| 9 | mouse | 20623 | Snrk | SNF related kinase | XM_006512000.3 | 82.1% | 88.8% | (many diffs) |
| 10 | mouse | 20623 | Snrk | SNF related kinase | XM_006512002.3 | 82.1% | 88.8% | (many diffs) |
| 11 | mouse | 20623 | Snrk | SNF related kinase | XM_006512003.3 | 82.1% | 88.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2364
- ORF length:
- 2295
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggcaggattt aagcgagggt atgatggaaa gattgctggg ttatatgatc 121 tggataaaac cttgggtcga ggccattttg ccgtggttaa acttgccagg catgtcttta 181 cgggtgaaaa ggtggcagta aaagttattg acaagacaaa actggacact ctagctactg 241 gtcatctttt ccaggaagtg agatgcatga aactagtgca gcatcctaac atcgtccgcc 301 tttatgaagt tattgacacc cagaccaaac tatatcttat tctagaactt ggggatggag 361 gagatatgtt tgattatata atgaaacatg aggagggtct taatgaagac ttggccaaga 421 agtattttgc tcagatagtt catgctatat cttattgcca taaactccat gtggttcaca 481 gagacttaaa accagagaat gtagtcttct ttgaaaaaca aggtcttgta aagttgacag 541 actttgggtt cagcaacaaa tttcaaccag ggaagaagct cactacaagc tgtggatctc 601 ttgcatattc cgctccagaa attctgcttg gtgatgagta tgatgcacct gcagtagata 661 tttggagtct gggagtgatc cttttcatgt tggtgtgtgg gcagccgccc tttcaagaag 721 ccaatgacag tgaaacactg acaatgatca tggattgcaa atatacagta ccatcccatg 781 tgtctaaaga gtgtaaagac ctaatcacac ggatgctaca gagagatccc aagagaaggg 841 cttatttaga agagattgaa aatcatcctt ggcttcaggg agtggaccct tcaccagcta 901 caaagtataa cattcccctt gtgtcataca aaaatctctc ggaagaggag cacaacagca 961 tcattcagcg catggtgctt ggggacatag cggatcgaga cgccattgta gaagccctgg 1021 aaaccaacag gtataaccat atcacagcca catacttcct gctggctgaa aggatcctga 1081 gagaaaagca agagaaagaa atacagacca gatctgcaag cccgagcaat atcaaggccc 1141 agtttaggca gtcatggcca accaaaattg atgtacccca ggaccttgag gatgacctca 1201 cggccactcc tttgtcccac gcgactgtcc ctcagtctcc tgctcgggct gctgacagtg 1261 tcctcaatgg ccacaggagc aaaggcctgt gtgactcagc taagaaagat gacctccctg 1321 agttggctgg accagcactc tctacggtgc cacccgcaag cttaaaaccc acagccagtg 1381 ggcggaagtg tctgttcagg gtggaagaag atgaagagga agatgaggag gacaagaaac 1441 ccatgtccct ctcaacacaa gtggttttgc gccggaagcc atctgtaacc aaccgcctga 1501 catccaggaa gagtgcgccc gtcgtcaacc agatctttga ggaaggggaa tctgacgatg 1561 agtttgacat ggatgagaat ctgcctccca agttgagcag gttaaagatg aatatagctt 1621 ctccaggtac agttcacaaa cgctaccacc ggaggaaaag tcagggccgg ggctccagct 1681 gcagtagttc ggagaccagt gatgatgatt ctgaaagccg gcggcggctc gataaagata 1741 gcgggttcac ctactcctgg caccgacggg atagcagcga ggggccccct ggcagtgagg 1801 gggatggcgg gggccagagc aagccaagca atgccagtgg aggggtggac aaggccagcc 1861 ccagtgagaa caatgctggt gggggcagtc cctccagcgg ctcgggtggc aaccccacca 1921 atacatcggg taccacacgc cgctgtgccg gcccaagcaa ctccatgcag ctggcctctc 1981 gcagtgctgg ggagctcgtt gagagcctca aactcatgag cctctgcctc ggctcccagc 2041 ttcatgggag caccaagtac attattgatc cacagaatgg cttgtcattt tccagtgtga 2101 aagtccaaga gaaatCTACG TGGAAAATGT GCATTAGCTC CACAGGGAAT GCAGGGCAGG 2161 TCCCTGCAGT GGGCGGCATA AAGTTTTTCT CTGACCACAT GGCAGATACC ACCACTGAAT 2221 TGGAACGGAT AAGGAGCAAG AACCTAAAAA ATAACGTGCT GCAGCTACCT CTGTGCGAAA 2281 AGACCATCTC TGTGAACATC CAGCGGAACC CTAAGGAGGG GCTGCTGTGC GCATCCAGCC 2341 CAGCCAGCTG TTGCCATGTC ATCTTGCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA 2401 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 2461 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAC CTCGCCGCGT 2521 CTCCTGACAT AATACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 2581 att