Transcript: Human NM_001330750.1

Homo sapiens SNF related kinase (SNRK), transcript variant 3, mRNA.

Source:
NCBI, updated 2019-08-06
Taxon:
Homo sapiens (human)
Gene:
SNRK (54861)
Length:
4479
CDS:
194..1873

Additional Resources:

NCBI RefSeq record:
NM_001330750.1
NBCI Gene record:
SNRK (54861)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

Clone ID Target Seq Vector PAM Seq Cut Position Cut % of CDS Length Exon On Target Score[?] Other Matching Genes Orig. Target Gene[?] Notes Addgene[?]
1 BRDN0001146508 GGCATTGACGTACTCTACAA pXPR_003 TGG 322 19% 3 0.6966 SNRK SNRK 76732
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shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001330750.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000196564 GAGCGTAACATCTGTATTAAA pLKO.1 3642 3UTR 100% 15.000 21.000 N SNRK n/a
2 TRCN0000315107 GGGCGTTAGGAGCAATTATTT pLKO_005 1967 3UTR 100% 15.000 21.000 N SNRK n/a
3 TRCN0000001954 GCAATATCAAGGCCCAGTTTA pLKO.1 633 CDS 100% 13.200 18.480 N SNRK n/a
4 TRCN0000382255 ATATCTGGAACCTCTTATAAA pLKO_005 2137 3UTR 100% 15.000 10.500 N SNRK n/a
5 TRCN0000194790 CTCTGCTACTTTAGGATAAAT pLKO.1 3368 3UTR 100% 15.000 10.500 N SNRK n/a
6 TRCN0000001953 CACTGAATTGGAACGGATAAA pLKO.1 1720 CDS 100% 13.200 9.240 N SNRK n/a
7 TRCN0000315038 CACTGAATTGGAACGGATAAA pLKO_005 1720 CDS 100% 13.200 9.240 N SNRK n/a
8 TRCN0000195348 CCCAAGTTGAGCAGGTTAAAG pLKO.1 1094 CDS 100% 13.200 9.240 N SNRK n/a
9 TRCN0000315037 CCCAAGTTGAGCAGGTTAAAG pLKO_005 1094 CDS 100% 13.200 9.240 N SNRK n/a
10 TRCN0000001951 GAGCAGGTTAAAGATGAATAT pLKO.1 1102 CDS 100% 13.200 9.240 N SNRK n/a
11 TRCN0000315105 GAGCAGGTTAAAGATGAATAT pLKO_005 1102 CDS 100% 13.200 9.240 N SNRK n/a
12 TRCN0000001955 CTAAAGAGTGTAAAGACCTAA pLKO.1 291 CDS 100% 4.950 3.465 N SNRK n/a
13 TRCN0000001952 GCACTTAACCTAGAGAGAGAA pLKO.1 2184 3UTR 100% 4.950 3.465 N SNRK n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001330750.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 TRCN0000489252 GAAATGTCCACACCCCCCCTTTTC pLX_317 14.4% 73% 73% V5 0_1ins618 n/a
2 TRCN0000488970 TGCTAGTCTATTTTCAGTACCAGA pLX_317 15.5% 72.9% 73% V5 (not translated due to prior stop codon) 0_1ins618;1677_1678insTGATC n/a
3 ccsbBroadEn_08424 pDONR223 100% 72.8% 72.6% None (many diffs) n/a
4 ccsbBroad304_08424 pLX_304 0% 72.8% 72.6% V5 (many diffs) n/a
5 TRCN0000475651 CCTCGCCGCGTCTCCTGACATAAT pLX_317 11.1% 72.8% 72.6% V5 (many diffs) n/a
6 ccsbBroadEn_15082 pDONR223 67.1% 66.9% 6.7% None (many diffs) n/a
7 ccsbBroad304_15082 pLX_304 0% 66.9% 6.7% V5 (not translated due to prior stop codon) (many diffs) n/a
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