Transcript: Human NM_001100594.1

Homo sapiens SNF related kinase (SNRK), transcript variant 2, mRNA.

Source:
NCBI, updated 2019-08-04
Taxon:
Homo sapiens (human)
Gene:
SNRK (54861)
Length:
5174
CDS:
271..2568

Additional Resources:

NCBI RefSeq record:
NM_001100594.1
NBCI Gene record:
SNRK (54861)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

Clone ID Target Seq Vector PAM Seq Cut Position Cut % of CDS Length Exon On Target Score[?] Other Matching Genes Orig. Target Gene[?] Notes Addgene[?]
1 BRDN0001146508 GGCATTGACGTACTCTACAA pXPR_003 TGG 940 41% 4 0.6893 SNRK SNRK 76732
2 BRDN0001149393 TGTCTTTACGGGTGAAAAGG pXPR_003 TGG 121 5% 2 0.066 SNRK SNRK 76731
3 BRDN0001146665 TTATTGCCATAAACTCCATG pXPR_003 TGG 400 17% 2 -0.0001 SNRK SNRK 76733
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shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001100594.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000196564 GAGCGTAACATCTGTATTAAA pLKO.1 4337 3UTR 100% 15.000 21.000 N SNRK n/a
2 TRCN0000315107 GGGCGTTAGGAGCAATTATTT pLKO_005 2662 3UTR 100% 15.000 21.000 N SNRK n/a
3 TRCN0000001954 GCAATATCAAGGCCCAGTTTA pLKO.1 1328 CDS 100% 13.200 18.480 N SNRK n/a
4 TRCN0000361463 TCAGATAGTTCATGCTATATC pLKO_005 633 CDS 100% 13.200 18.480 N Snrk n/a
5 TRCN0000024290 GCTCAGATAGTTCATGCTATA pLKO.1 631 CDS 100% 10.800 15.120 N Snrk n/a
6 TRCN0000382255 ATATCTGGAACCTCTTATAAA pLKO_005 2832 3UTR 100% 15.000 10.500 N SNRK n/a
7 TRCN0000194790 CTCTGCTACTTTAGGATAAAT pLKO.1 4063 3UTR 100% 15.000 10.500 N SNRK n/a
8 TRCN0000001953 CACTGAATTGGAACGGATAAA pLKO.1 2415 CDS 100% 13.200 9.240 N SNRK n/a
9 TRCN0000315038 CACTGAATTGGAACGGATAAA pLKO_005 2415 CDS 100% 13.200 9.240 N SNRK n/a
10 TRCN0000195348 CCCAAGTTGAGCAGGTTAAAG pLKO.1 1789 CDS 100% 13.200 9.240 N SNRK n/a
11 TRCN0000315037 CCCAAGTTGAGCAGGTTAAAG pLKO_005 1789 CDS 100% 13.200 9.240 N SNRK n/a
12 TRCN0000315039 CCTAACATCGTCCGCCTTTAT pLKO_005 487 CDS 100% 13.200 9.240 N SNRK n/a
13 TRCN0000001951 GAGCAGGTTAAAGATGAATAT pLKO.1 1797 CDS 100% 13.200 9.240 N SNRK n/a
14 TRCN0000315105 GAGCAGGTTAAAGATGAATAT pLKO_005 1797 CDS 100% 13.200 9.240 N SNRK n/a
15 TRCN0000001955 CTAAAGAGTGTAAAGACCTAA pLKO.1 986 CDS 100% 4.950 3.465 N SNRK n/a
16 TRCN0000001952 GCACTTAACCTAGAGAGAGAA pLKO.1 2879 3UTR 100% 4.950 3.465 N SNRK n/a
17 TRCN0000196645 GAAAGATTGCTGGATTATATG pLKO.1 299 CDS 100% 13.200 7.920 N SNRK n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001100594.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 TRCN0000489252 GAAATGTCCACACCCCCCCTTTTC pLX_317 14.4% 99.9% 100% V5 16C>A n/a
2 TRCN0000488970 TGCTAGTCTATTTTCAGTACCAGA pLX_317 15.5% 99.7% 100% V5 (not translated due to prior stop codon) 2295_2296insTGATC n/a
3 ccsbBroadEn_08424 pDONR223 100% 99.7% 99.6% None (many diffs) n/a
4 ccsbBroad304_08424 pLX_304 0% 99.7% 99.6% V5 (many diffs) n/a
5 TRCN0000475651 CCTCGCCGCGTCTCCTGACATAAT pLX_317 11.1% 99.7% 99.6% V5 (many diffs) n/a
6 ccsbBroadEn_15082 pDONR223 67.1% 93.5% 33.7% None (many diffs) n/a
7 ccsbBroad304_15082 pLX_304 0% 93.5% 33.7% V5 (not translated due to prior stop codon) (many diffs) n/a
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