Transcript: Mouse NM_133741.2

Mus musculus SNF related kinase (Snrk), transcript variant 1, mRNA.

Source:
NCBI, updated 2017-05-27
Taxon:
Mus musculus (mouse)
Gene:
Snrk (20623)
Length:
4860
CDS:
300..2546

Additional Resources:

NCBI RefSeq record:
NM_133741.2
NBCI Gene record:
Snrk (20623)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

Clone ID Target Seq Vector PAM Seq Cut Position Cut % of CDS Length Exon On Target Score[?] Other Matching Genes Orig. Target Gene[?] Notes Addgene[?]
1 BRDN0001146665 TTATTGCCATAAACTCCATG pXPR_003 TGG 400 18% 3 0.0205 Snrk SNRK 76733
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shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_133741.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000024290 GCTCAGATAGTTCATGCTATA pLKO.1 660 CDS 100% 10.800 15.120 N Snrk n/a
2 TRCN0000361532 TATCTGGAACCCAACGTAAAT pLKO_005 2700 3UTR 100% 13.200 10.560 N Snrk n/a
3 TRCN0000361463 TCAGATAGTTCATGCTATATC pLKO_005 662 CDS 100% 13.200 9.240 N Snrk n/a
4 TRCN0000024289 CCAGCCACCAAGTATAACATT pLKO.1 1125 CDS 100% 5.625 3.938 N Snrk n/a
5 TRCN0000024292 CCTGGAAACCAACAGGTACAA pLKO.1 1247 CDS 100% 4.950 3.465 N Snrk n/a
6 TRCN0000221238 GCATGTCTTCACAGGTGAGAA pLKO.1 401 CDS 100% 4.950 3.465 N LOC241864 n/a
7 TRCN0000024291 CGAGTTTGACATGGACGAGAA pLKO.1 1790 CDS 100% 4.050 2.835 N Snrk n/a
8 TRCN0000361530 ATCACCAGCCACCAAGTATAA pLKO_005 1121 CDS 100% 13.200 7.920 N Snrk n/a
9 TRCN0000024293 GCAACATCAAGGCCCAGTTTA pLKO.1 1357 CDS 100% 13.200 7.920 N Snrk n/a
10 TRCN0000221234 CCCAACATTGTGCGTCTCTAT pLKO.1 516 CDS 100% 4.950 2.970 N LOC241864 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_133741.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 TRCN0000489252 GAAATGTCCACACCCCCCCTTTTC pLX_317 14.4% 82.4% 88.9% V5 (many diffs) n/a
2 TRCN0000488970 TGCTAGTCTATTTTCAGTACCAGA pLX_317 15.5% 82.3% 88.9% V5 (not translated due to prior stop codon) (many diffs) n/a
3 ccsbBroadEn_08424 pDONR223 100% 82.1% 88.8% None (many diffs) n/a
4 ccsbBroad304_08424 pLX_304 0% 82.1% 88.8% V5 (many diffs) n/a
5 TRCN0000475651 CCTCGCCGCGTCTCCTGACATAAT pLX_317 11.1% 82.1% 88.8% V5 (many diffs) n/a
6 ccsbBroadEn_15082 pDONR223 67.1% 77.8% 33.8% None (many diffs) n/a
7 ccsbBroad304_15082 pLX_304 0% 77.8% 33.8% V5 (not translated due to prior stop codon) (many diffs) n/a
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