Construct: ORF TRCN0000476145
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015863.1_s317c1
- Derived from:
- ccsbBroadEn_05500
- DNA Barcode:
- AGCCGGTTCGCAAAATTCCTTCCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ECT2L (345930)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000476145
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 345930 | ECT2L | epithelial cell transformin... | NM_001077706.2 | 100% | 100% | |
| 2 | human | 345930 | ECT2L | epithelial cell transformin... | NM_001195037.2 | 100% | 100% | |
| 3 | human | 345930 | ECT2L | epithelial cell transformin... | XM_006715472.3 | 95.5% | 95.5% | 1579_1704del |
| 4 | human | 345930 | ECT2L | epithelial cell transformin... | XM_011535795.2 | 95.5% | 95.5% | 1579_1704del |
| 5 | human | 345930 | ECT2L | epithelial cell transformin... | XM_017010828.1 | 95.5% | 95.5% | 1579_1704del |
| 6 | human | 345930 | ECT2L | epithelial cell transformin... | XM_017010829.1 | 92.2% | 92.1% | 1198_1199ins93;1486_1611del |
| 7 | human | 345930 | ECT2L | epithelial cell transformin... | XM_011535797.2 | 88.2% | 88.2% | 0_1ins207;1372_1497del |
| 8 | human | 345930 | ECT2L | epithelial cell transformin... | XM_017010830.1 | 59.1% | 58.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2781
- ORF length:
- 2712
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggagagcttc cacaccagat ttagtgcctg gacacctttt agcaacaagt 121 cattaaatag acagctcttt caggaaagag tggctcttat aagtcattgg tttgacctct 181 ggactaacaa gcaacgtcaa gaattcttat tcgcaatttt tttaagatgc actaaatcac 241 aattaaggtt tgtccaagac tggttttcag aaaggatgca agtggccaaa gtggacttct 301 ctacagtgtt accacgcttc atttctctat atatcttttc ctttttgagt ccgaaagatt 361 tgtgtgccgc tgcccaagtc agctggccct ggaagttttt aactgaacag gattgcttat 421 ggatgcccaa atgcgttaag ttcggatggt ttctgcccta tactccaaca gataatgagt 481 atggtgcttg gaagcgccat tacattgctt gtgtgtccca cttagactgg ctgacaccta 541 gggaggctgc tgctacttat gggacgctga atgaacccaa aacagaagat gaggaactac 601 tggagagaca aagagaaaag tgcctgagga aaagaatttg ggagaaaatt gcactacgta 661 agaaggagtt attcaaagtt cgaccccctt gggtgagtgg aacttgctgc tctagcgtgc 721 taaagcccag atgccaacca cgcctctccc agactgtaag ggagcgagtg ggattacatg 781 aagctttgga gaaacagctt gttttgacat cgttagaaac cttgcccaag cgaagcaata 841 tttctggaag ccattcctac cctttattat caaagaaaaa ttggcatgga gttcataaaa 901 atgatgacag atcttcatat gctctccggc cacacttcat gttaatatca tcccggattc 961 ctgcgtatga gatggtgatg gagagtgtga aggctggtgt tgtttctgtg gtatatgaac 1021 acagcgtaac cttggaaagc cttctgtatc ttatagaaaa agctctggat gggcagaagg 1081 cacagagcat cggaatattt agcgatggag acagcagaga aatcaattta ctccaaggct 1141 ataaaattgg tgttaaaaat ttactgaggc ctgaagtgag agatttctgg gagaaattag 1201 gaagctatgt ggccactgaa gaagaagggg gtcacgtgga cttcttcgtg ccccttggag 1261 catcagaggc aggaattgaa gttctttccc agctgtctca actaactggc acgttcttta 1321 cggcccccac tgggattgca actggctctt accagcacat tcttagtgat tggctgggat 1381 cccaatgggg aaaggccccc tcttccatct acttctgcga atcgaagcta cagacgtggt 1441 ccagcttcac agacttccta gaagaaacct tgaaaacagt aaggaagcag ctgtatcctt 1501 tcttcaagga actgcagaag agcatcagtg gcaggatgat agggcagttt atgtttgaca 1561 ccatgggtat gaccaacatt ctaaacaacc aagatactgc gcaagctctg gcagatggat 1621 tgatggagtt gtcaaaagaa gattctgaaa gaaatgttgt agaagacaat tcttgggaca 1681 caaagtccag gctcagcaaa aatgatttaa attttgaagc actgattaat ctggagagaa 1741 tactccagaa ggactcagca gaaaagcgag ctagagttgt cagagaactc ttacagagtg 1801 agagaaaata cgtgcagata ctggaaattg tgagagatgt ttatgtcgca ccactgaaag 1861 cagcattgtc atcaaacaga gcgattctga gtgctgccaa tatccagatc attttctgtg 1921 acattctaca gattttaagt ctcaacaggc agtttctaga taacctgaga gacagactgc 1981 aggaatgggg cccagctcac tgtgtgggag aaatagtcac gaagtttgga agccagttaa 2041 acacatatac caatttcttc aacaattacc ctgtcattct gaaaactatt gagaagtgca 2101 gagaaatgat accagcattc cgaactttcc tgaagaggca tgataagacc attgttacca 2161 aaatgctgag cctgccagag ctgctgctgt acccatcccg aagatttgaa gaatacctta 2221 atcttctcta cgctgtcagg cttcataccc ctgcagagca tgttgaccgt ggggacttga 2281 ccactgcaat tgaccaaatc aaaaaatata aaggttatat agatcagatg aagcaaaaca 2341 tcactatgaa ggatcatctg tcagatatac agagaatcat ctggggatgc ccTACTCTAT 2401 CAGAAGTAAA CAGATATCTG ATTAGGGTAC AAGATGTAGC CCAACTTCAT TGCTGTGATG 2461 AAGAAATAAG TTTCTCTTTA AGGCTCTATG AACACATCCA TGATCTCAGC CTTTTCCTCT 2521 TCAATGATGC CCTGCTCGTT TCTAGTCGGG GCACATCTCA CACTCCATTT GAGAGGACTT 2581 CAAAAACAAC CTACCAGTTC ATTGCATCAG TGGCCCTTCA TCGGTTACTC ATAGAAAATA 2641 TTCCAGATTC CAAGTATGTC AAGAATGCAT TTATTCTTCA GGGTCCAAAA TATAAATGGA 2701 TTTGTGCTAC AGAAATAGAG GATGATAAGT TCCTATGGCT GTCAGTACTT CGAAATGCAA 2761 TCAAAAGCAG TATGGAGAAG TTGCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 2821 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 2881 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGAAGCC GGTTCGCAAA 2941 ATTCCTTCCC ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt