Construct: ORF TRCN0000476325

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF008681.1_s317c1
Derived from:
ccsbBroadEn_01248
DNA Barcode:
GTCACTGTTAGTTGAGAACCACAG
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
POU6F1 (5463)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000476325
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 5463 POU6F1 POU class 6 homeobox 1 XM_017019529.1 100% 100%
2 human 5463 POU6F1 POU class 6 homeobox 1 XM_017019518.1 52% 49.9% (many diffs)
3 human 5463 POU6F1 POU class 6 homeobox 1 XM_017019517.1 51.8% 49.5% (many diffs)
4 human 5463 POU6F1 POU class 6 homeobox 1 XM_017019516.1 49.3% 49.3% 1_927del
5 human 5463 POU6F1 POU class 6 homeobox 1 NM_001330422.2 49.2% 49.2% 1_930del
6 human 5463 POU6F1 POU class 6 homeobox 1 XM_017019514.1 49.2% 49.2% 1_930del
7 human 5463 POU6F1 POU class 6 homeobox 1 XM_017019513.1 49.1% 49% 1_927del;1319_1324delGCCTAG
8 human 5463 POU6F1 POU class 6 homeobox 1 XM_017019508.1 49.1% 48.9% 1_930del;1322_1327delGCCTAG
9 human 5463 POU6F1 POU class 6 homeobox 1 XM_017019510.2 49.1% 48.9% 1_930del;1322_1327delGCCTAG
10 human 5463 POU6F1 POU class 6 homeobox 1 XM_017019511.1 49.1% 48.9% 1_930del;1322_1327delGCCTAG
11 human 5463 POU6F1 POU class 6 homeobox 1 XM_017019512.2 49.1% 48.9% 1_930del;1322_1327delGCCTAG
12 human 5463 POU6F1 POU class 6 homeobox 1 XM_024449026.1 49.1% 48.9% 1_930del;1322_1327delGCCTAG
13 human 5463 POU6F1 POU class 6 homeobox 1 XM_024449027.1 22.1% 20.3% (many diffs)
14 human 5463 POU6F1 POU class 6 homeobox 1 XM_017019519.1 21.4% 21.2% 1_930del;1321_1328delAGTTCGAG;1332_1333ins509
15 human 5463 POU6F1 POU class 6 homeobox 1 NR_160430.1 17.2% 1_1015del;1919_5232del
16 human 5463 POU6F1 POU class 6 homeobox 1 NR_147049.2 17% 1_1092del;1996_5309del
17 human 5463 POU6F1 POU class 6 homeobox 1 NR_147052.2 16.6% 1_1214del;2118_5431del
18 human 5463 POU6F1 POU class 6 homeobox 1 NR_147050.2 16.5% 1_1233del;2137_5450del
19 human 5463 POU6F1 POU class 6 homeobox 1 NR_147046.2 16.4% (many diffs)
20 human 5463 POU6F1 POU class 6 homeobox 1 NR_147048.2 16.4% (many diffs)
21 human 5463 POU6F1 POU class 6 homeobox 1 NR_147051.2 16.3% 1_1301del;2205_5518del
22 human 5463 POU6F1 POU class 6 homeobox 1 NR_147047.2 16.3% 1_1306del;2210_5523del
23 human 5463 POU6F1 POU class 6 homeobox 1 NR_026893.3 16.3% 1_1309del;2213_5526del
24 human 5463 POU6F1 POU class 6 homeobox 1 NR_160429.1 15.6% (many diffs)
25 human 5463 POU6F1 POU class 6 homeobox 1 XR_001748774.1 14.2% 1_2140del;3044_6357del
26 mouse 19009 Pou6f1 POU domain, class 6, transc... XM_030248419.1 79.9% 86.7% (many diffs)
27 mouse 19009 Pou6f1 POU domain, class 6, transc... XM_030248418.1 46% 49.9% (many diffs)
28 mouse 19009 Pou6f1 POU domain, class 6, transc... NM_001304965.1 45.9% 49.8% (many diffs)
29 mouse 19009 Pou6f1 POU domain, class 6, transc... XM_030248417.1 44.8% 48.5% (many diffs)
30 mouse 19009 Pou6f1 POU domain, class 6, transc... XM_006520609.4 43.4% 47.1% (many diffs)
31 mouse 19009 Pou6f1 POU domain, class 6, transc... XM_006520608.4 43.4% 47% (many diffs)
32 mouse 19009 Pou6f1 POU domain, class 6, transc... XM_030248416.1 43.4% 47% (many diffs)
33 mouse 19009 Pou6f1 POU domain, class 6, transc... XM_017316509.2 20.6% 19.2% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
972
ORF length:
903
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcaccat gccagggatc agcagtcaga tcctcaccaa tgctcaggga caggttattg
121gaacccttcc atgggtagtg aactcagcta gtgtggcggc cccagcacca gcccaaagcc
181tgcaggtcca ggccgtgacc ccccagctgt tgttgaacgc ccagggccag gtgattgcga
241ccctggctag cagccccctg cctccacctg tggctgtccg gaagccaagc acacctgagt
301cccctgctaa gagtgaggtg cagcccatcc agcccacacc aaccgtgccc cagcctgctg
361tggtcattgc cagcccagct ccagccgcca agccatctgc ctctgctcct atcccaatta
421cctgctcaga gacccccacc gtcagccagt tggtgtccaa gccacatact ccaagtctgg
481atgaggatgg gatcaactta gaagagatcc gggagtttgc caagaacttt aagatccggc
541ggctctcgct gggccttaca cagacccagg tgggtcaggc tctgactgca acggAAGGTC
601CAGCCTACAG CCAGTCAGCC ATCTGCCGGT TCGAGAAGCT AGACATCACA CCCAAGAGTG
661CCCAGAAGCT AAAGCCGGTG CTGGAAAAGT GGCTAAACGA AGCTGAACTG CGGAACCAGG
721AAGGCCAGCA GAACCTGATG GAGTTTGTGG GAGGCGAGCC CTCCAAGAAA CGCAAACGCC
781GCACCTCCTT CACCCCCCAG GCCATAGAGG CTCTCAATGC CTATTTTGAG AAGAACCCAC
841TGCCCACAGG CCAGGAGATC ACTGAAATTG CTAAGGAGCT CAACTACGAC CGTGAGGTAG
901TGCGGGTCTG GTTCTGCAAT CGGCGCCAGA CGCTCAAGAA CACCAGCAAG CTGAACGTCT
961TTCAGATCCC TTTGCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA
1021ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT
1081CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGAGTC ACTGTTAGTT GAGAACCACA
1141GACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t

Download FASTA (ORF) (Full)