Construct: ORF TRCN0000476347

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF003958.1_s317c1
Derived from:
ccsbBroadEn_01233
DNA Barcode:
CCATTTCCAACTATGATTTCGGGC
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Early stop codon detected

Originally Annotated References:

Gene:
SEPTIN4 (5414)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000476347
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 5414 SEPTIN4 septin 4 NM_004574.4 90.2% 84.9% 1277_1278ins155
2 human 5414 SEPTIN4 septin 4 NM_001198713.1 88.1% 80.8% (many diffs)
3 human 5414 SEPTIN4 septin 4 NM_001368772.1 88% 81.2% (many diffs)
4 human 5414 SEPTIN4 septin 4 XM_006721949.3 87.6% 80.9% (many diffs)
5 human 5414 SEPTIN4 septin 4 NM_080416.3 86.5% 80.9% 1_2delATins59;1220_1221ins155
6 human 5414 SEPTIN4 septin 4 XR_002958032.1 86.2% (many diffs)
7 human 5414 SEPTIN4 septin 4 NM_001256782.1 85.9% 79.6% (many diffs)
8 human 5414 SEPTIN4 septin 4 XR_002958031.1 84% (many diffs)
9 human 5414 SEPTIN4 septin 4 XR_002958037.1 82.6% (many diffs)
10 human 5414 SEPTIN4 septin 4 NR_104196.1 77% 1_293del;1883_2063del
11 human 5414 SEPTIN4 septin 4 XR_002958034.1 76.2% (many diffs)
12 human 5414 SEPTIN4 septin 4 XR_002958039.1 75.9% (many diffs)
13 human 5414 SEPTIN4 septin 4 XR_002958036.1 72.8% (many diffs)
14 human 5414 SEPTIN4 septin 4 XR_002958033.1 72.5% (many diffs)
15 human 5414 SEPTIN4 septin 4 NR_104197.1 72% (many diffs)
16 human 5414 SEPTIN4 septin 4 NM_001363803.1 71.5% 65.4% 59_60ins297;980_981ins155
17 human 5414 SEPTIN4 septin 4 XM_006721950.4 71.5% 62.2% (many diffs)
18 human 5414 SEPTIN4 septin 4 XM_006721952.3 67.9% 61.5% 0_1ins346;2_3insTCCTCTGA;923_924ins155
19 human 5414 SEPTIN4 septin 4 NR_037155.2 65.3% 1_293del;649_1016del;2251_2431del
20 human 5414 SEPTIN4 septin 4 NM_001256822.1 62.4% 56% 0_1ins441;836_837ins155
21 human 5414 SEPTIN4 septin 4 XM_006721954.3 62.4% 56% 0_1ins441;836_837ins155
22 human 5414 SEPTIN4 septin 4 XM_006721955.3 62.4% 56% 0_1ins441;836_837ins155
23 human 5414 SEPTIN4 septin 4 XM_011524911.1 62.4% 56% 0_1ins441;836_837ins155
24 human 5414 SEPTIN4 septin 4 XM_011524912.2 62.4% 56% 0_1ins441;836_837ins155
25 human 5414 SEPTIN4 septin 4 XM_024450807.1 62.4% 56% 0_1ins441;836_837ins155
26 human 5414 SEPTIN4 septin 4 XM_024450808.1 62.4% 56% 0_1ins441;836_837ins155
27 human 5414 SEPTIN4 septin 4 XR_002958040.1 53% (many diffs)
28 human 5414 SEPTIN4 septin 4 NM_080415.3 49% 52.1% (many diffs)
29 human 5414 SEPTIN4 septin 4 NM_001368771.2 44.8% 40% (many diffs)
30 human 5414 SEPTIN4 septin 4 XR_002958035.1 38.1% (many diffs)
31 human 5414 SEPTIN4 septin 4 XR_002958038.1 33% 1_2289del;2396_2397ins250;3629_3802del
32 mouse 18952 Sept4 septin 4 NM_011129.2 80.9% 78.2% (many diffs)
33 mouse 18952 Sept4 septin 4 XM_006532497.3 78.8% 75.3% (many diffs)
34 mouse 18952 Sept4 septin 4 NM_001368776.1 77.9% 73.9% (many diffs)
35 mouse 18952 Sept4 septin 4 NM_001361936.1 77.5% 74.7% (many diffs)
36 mouse 18952 Sept4 septin 4 NM_001284394.1 72.6% 82.2% (many diffs)
37 mouse 18952 Sept4 septin 4 XM_030245664.1 70.5% 79.1% (many diffs)
38 mouse 18952 Sept4 septin 4 NM_001368779.1 69.7% 77.6% (many diffs)
39 mouse 18952 Sept4 septin 4 NM_001368778.1 69.2% 78.4% (many diffs)
40 mouse 18952 Sept4 septin 4 NM_001284392.1 65.3% 64.3% (many diffs)
41 mouse 18952 Sept4 septin 4 XM_030245665.1 62.9% 61.6% (many diffs)
42 mouse 18952 Sept4 septin 4 NM_001368777.1 62% 60.8% (many diffs)
43 mouse 18952 Sept4 septin 4 XM_030245666.1 57% 67.4% (many diffs)
44 mouse 18952 Sept4 septin 4 XM_030245667.1 56.9% 55.3% (many diffs)
45 mouse 18952 Sept4 septin 4 NM_001284398.1 53.7% 63.6% (many diffs)
46 mouse 18952 Sept4 septin 4 XM_006532493.2 41.3% 38% (many diffs)
47 mouse 18952 Sept4 septin 4 NM_001368775.1 37% 38.6% (many diffs)
48 mouse 18952 Sept4 septin 4 XR_388364.2 34.9% (many diffs)
49 mouse 18952 Sept4 septin 4 XR_001779919.2 33.5% (many diffs)
50 mouse 18952 Sept4 septin 4 XM_030245663.1 25.4% 23% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
1506
ORF length:
1437
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcaccat ggaccgttca ctgggatggc aagggaattc tgtccctgag gacaggactg
121aagctgggat caagcgtttc ctggaggaca ccacggatga tggagaactg agcaagttcg
181tgaaggattt ctcaggaaat gcgagctgcc acccaccaga ggctaagacc tgggcatcca
241ggccccaagt cccggagcca aggccccagg ccccggacct ctatgatgat gacctggagt
301tcagaccccc ctcgcggccc cagtcctctg acaaccagca gtacttctgt gccccagccc
361ctctcagccc atctgccagg ccccgcagcc catggggcaa gcttgatccc tatgattcct
421ctgaggatga caaggagtat gtgggctttg caaccctccc caaccaagtc caccgaaagt
481ccgtgaagaa aggctttgac tttaccctca tggtggcagg agagtctggc ctgggcaaat
541ccacacttgt caatagcctc ttcctcactg atctgtaccg ggaccggaaa cttcttggtg
601ctgaagagag gatcatgcaa actgtggaga tcactaagca tgcagtggac atagaagaga
661agggtgtgag gctgcggctc accattgtgg acacaccagg ttttggggat gcagtcaaca
721acacagagtg ctggaagcct gtggcagaat acattgatca gcagtttgag cagtatttcc
781gagacgagag tggcctgaac cgaaagaaca tccaagacaa cagggtgcac tgctgcctgt
841acttcatctc acccttcggc catgggctcc ggccattgga tgttgaattc atgaaggccc
901tgcatcagcg ggtcaacatc gtgcctatcc tggctaaggc agacacactg acacctcccg
961aagtggacca caagaaacgc aaaatccggg aggagattga gcattttgga atcaagatct
1021atcaattccc agactgtgac tctgatgagg atgaggactt caaattgcag gaccaagccc
1081taaaggaaag catcccattt gcagtaattg gcagcaacac tgtagtagag gccagagggc
1141ggcgagttcg gggtcgactc tacccctggg gcatcgtgga agtggaaaac ccagggcact
1201gcgactttgt gaagctgagg acaatgctgg tacgtaccca catgcaggac cTGAAGGATG
1261TGACACGGGA GACACATTAT GAGAACTACC GGGCACAGTG CATCCAGAGC ATGACCCGCC
1321TGGTGGTGAA GGAACGGAAT CGCAAGTATG ACCAGAAGCC AGGACAAAGC TGGCAGGGGG
1381AGATCCCAAG CCTAGCCTTG GGTGAGACCA AGCCCTACTT TTGTTCTTCT ATAGGCCCTG
1441GGCTCAATCT AAGCGGGTGC TGGGGTCCTC CTCGCCTTAT CAACCCTTTT CTCCCTTTAG
1501CAAACTGACT CGGGAAAGTG GTACCGACTT CCCCATCCCT GCTGTCCCAC CAGGGACAGA
1561TCCAGAAACT GAGAAGCTTA TCCGAGAGAA AGATGAGGAG CTGCGGCGGA TGCAGGAGAT
1621GCTACACAAA ATACAAAAAC AGATGAAGGA GAACTATTTG CCAACTTTCT TGTACAAAGT
1681GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG
1741AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA
1801CGACCATTTC CAACTATGAT TTCGGGCACG CGTTAAGTCg acaatcaacc tctggattac
1861aaaatttgtg aaagatt

Download FASTA (ORF) (Full)

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