Construct: ORF TRCN0000476448

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF011102.1_s317c1
Derived from:
ccsbBroadEn_13327
DNA Barcode:
GTATAATACAGCTCGGATCTAGCC
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
USP32P1 (162632)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000476448
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 162632 USP32P1 ubiquitin specific peptidas... NR_003190.2 5.4% 1_1364del;1369G>A;1578_3901del
2 human 84669 USP32 ubiquitin specific peptidas... XM_017025234.1 5.2% 4.5% (many diffs)
3 human 84669 USP32 ubiquitin specific peptidas... XM_011525379.3 5% 4.4% (many diffs)
4 human 9098 USP6 ubiquitin specific peptidase 6 XM_011524057.2 4.9% 4.4% (many diffs)
5 human 9098 USP6 ubiquitin specific peptidase 6 NM_001304284.2 4.9% 4.3% (many diffs)
6 human 9098 USP6 ubiquitin specific peptidase 6 NM_004505.4 4.9% 4.3% (many diffs)
7 human 9098 USP6 ubiquitin specific peptidase 6 XM_011524050.1 4.9% 4.3% (many diffs)
8 human 9098 USP6 ubiquitin specific peptidase 6 XM_011524051.2 4.9% 4.3% (many diffs)
9 human 9098 USP6 ubiquitin specific peptidase 6 XM_011524052.2 4.9% 4.3% (many diffs)
10 human 9098 USP6 ubiquitin specific peptidase 6 XM_011524053.2 4.9% 4.3% (many diffs)
11 human 9098 USP6 ubiquitin specific peptidase 6 XM_011524054.2 4.9% 4.3% (many diffs)
12 human 9098 USP6 ubiquitin specific peptidase 6 XM_011524055.2 4.9% 4.3% (many diffs)
13 human 9098 USP6 ubiquitin specific peptidase 6 XM_011524056.2 4.9% 4.3% (many diffs)
14 human 9098 USP6 ubiquitin specific peptidase 6 XM_017025290.1 4.9% 4.3% (many diffs)
15 human 347716 USP32P3 ubiquitin specific peptidas... NR_146076.1 4.6% (many diffs)
16 human 84669 USP32 ubiquitin specific peptidas... XM_017025233.1 4.3% 3.7% (many diffs)
17 human 84669 USP32 ubiquitin specific peptidas... XM_011525378.1 4.2% 3.7% (many diffs)
18 human 220594 USP32P2 ubiquitin specific peptidas... NR_003554.1 4.2% (many diffs)
19 human 84669 USP32 ubiquitin specific peptidas... XM_011525376.1 4.2% 3.6% (many diffs)
20 human 84669 USP32 ubiquitin specific peptidas... NM_032582.4 4.1% 3.6% (many diffs)
21 human 84669 USP32 ubiquitin specific peptidas... XM_011525375.1 4.1% 3.6% (many diffs)
22 human 84669 USP32 ubiquitin specific peptidas... XM_011525374.1 4.1% 3.6% (many diffs)
23 human 84669 USP32 ubiquitin specific peptidas... XM_011525373.1 4.1% 3.6% (many diffs)
24 human 84669 USP32 ubiquitin specific peptidas... XM_011525372.1 4.1% 3.6% (many diffs)
25 human 84669 USP32 ubiquitin specific peptidas... XM_011525371.1 4.1% 3.6% (many diffs)
26 mouse 237898 Usp32 ubiquitin specific peptidas... NM_001029934.1 3.9% 3.4% (many diffs)
27 mouse 237898 Usp32 ubiquitin specific peptidas... XM_011248990.1 3.9% 3.3% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
116
ORF end:
329
ORF length:
213
Sequence:
1GGATCTTGGT TCATTCTCAA GCCTCAGACA GTGGTTCAAA GTTTTTTTCT TCCATTTCAG
61GTGTCGTGAG GCTAGCATCG ATTGATCAAC AAGTTTGTAC AAAAAAGTTG GCACCATGCA
121GGGGGTGGGC AGCCAACCAG AGCTGGTCAC TCCTCAGGAC CATGAGGTAG CTTTGGCCAA
181TGGATTCCTT TATGAGCATG AAGCATATGG CAATGGCTAT AGCAATGGTC AGCTTGGAAA
241CCACAGTGAA GAAGACAGCA CTGATGACCA AAGAGAAGAC ACTCATATTA AGCCTATTTA
301TATGCAATTT CATGCCATTC AGGAATTCTT GCCAACTTTC TTGTACAAAG TGGTTGATAT
361CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC
421GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGAGTATAA
481TACAGCTCGG ATCTAGCCAC GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt
541gaaagatt

Download FASTA (ORF) (Full)

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