Construct: ORF TRCN0000476448
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011102.1_s317c1
- Derived from:
- ccsbBroadEn_13327
- DNA Barcode:
- GTATAATACAGCTCGGATCTAGCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- USP32P1 (162632)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000476448
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 162632 | USP32P1 | ubiquitin specific peptidas... | NR_003190.2 | 5.4% | 1_1364del;1369G>A;1578_3901del | |
| 2 | human | 84669 | USP32 | ubiquitin specific peptidas... | XM_017025234.1 | 5.2% | 4.5% | (many diffs) |
| 3 | human | 84669 | USP32 | ubiquitin specific peptidas... | XM_011525379.3 | 5% | 4.4% | (many diffs) |
| 4 | human | 9098 | USP6 | ubiquitin specific peptidase 6 | XM_011524057.2 | 4.9% | 4.4% | (many diffs) |
| 5 | human | 9098 | USP6 | ubiquitin specific peptidase 6 | NM_001304284.2 | 4.9% | 4.3% | (many diffs) |
| 6 | human | 9098 | USP6 | ubiquitin specific peptidase 6 | NM_004505.4 | 4.9% | 4.3% | (many diffs) |
| 7 | human | 9098 | USP6 | ubiquitin specific peptidase 6 | XM_011524050.1 | 4.9% | 4.3% | (many diffs) |
| 8 | human | 9098 | USP6 | ubiquitin specific peptidase 6 | XM_011524051.2 | 4.9% | 4.3% | (many diffs) |
| 9 | human | 9098 | USP6 | ubiquitin specific peptidase 6 | XM_011524052.2 | 4.9% | 4.3% | (many diffs) |
| 10 | human | 9098 | USP6 | ubiquitin specific peptidase 6 | XM_011524053.2 | 4.9% | 4.3% | (many diffs) |
| 11 | human | 9098 | USP6 | ubiquitin specific peptidase 6 | XM_011524054.2 | 4.9% | 4.3% | (many diffs) |
| 12 | human | 9098 | USP6 | ubiquitin specific peptidase 6 | XM_011524055.2 | 4.9% | 4.3% | (many diffs) |
| 13 | human | 9098 | USP6 | ubiquitin specific peptidase 6 | XM_011524056.2 | 4.9% | 4.3% | (many diffs) |
| 14 | human | 9098 | USP6 | ubiquitin specific peptidase 6 | XM_017025290.1 | 4.9% | 4.3% | (many diffs) |
| 15 | human | 347716 | USP32P3 | ubiquitin specific peptidas... | NR_146076.1 | 4.6% | (many diffs) | |
| 16 | human | 84669 | USP32 | ubiquitin specific peptidas... | XM_017025233.1 | 4.3% | 3.7% | (many diffs) |
| 17 | human | 84669 | USP32 | ubiquitin specific peptidas... | XM_011525378.1 | 4.2% | 3.7% | (many diffs) |
| 18 | human | 220594 | USP32P2 | ubiquitin specific peptidas... | NR_003554.1 | 4.2% | (many diffs) | |
| 19 | human | 84669 | USP32 | ubiquitin specific peptidas... | XM_011525376.1 | 4.2% | 3.6% | (many diffs) |
| 20 | human | 84669 | USP32 | ubiquitin specific peptidas... | NM_032582.4 | 4.1% | 3.6% | (many diffs) |
| 21 | human | 84669 | USP32 | ubiquitin specific peptidas... | XM_011525375.1 | 4.1% | 3.6% | (many diffs) |
| 22 | human | 84669 | USP32 | ubiquitin specific peptidas... | XM_011525374.1 | 4.1% | 3.6% | (many diffs) |
| 23 | human | 84669 | USP32 | ubiquitin specific peptidas... | XM_011525373.1 | 4.1% | 3.6% | (many diffs) |
| 24 | human | 84669 | USP32 | ubiquitin specific peptidas... | XM_011525372.1 | 4.1% | 3.6% | (many diffs) |
| 25 | human | 84669 | USP32 | ubiquitin specific peptidas... | XM_011525371.1 | 4.1% | 3.6% | (many diffs) |
| 26 | mouse | 237898 | Usp32 | ubiquitin specific peptidas... | NM_001029934.1 | 3.9% | 3.4% | (many diffs) |
| 27 | mouse | 237898 | Usp32 | ubiquitin specific peptidas... | XM_011248990.1 | 3.9% | 3.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 116
- ORF end:
- 329
- ORF length:
- 213
- Sequence:
-
1 GGATCTTGGT TCATTCTCAA GCCTCAGACA GTGGTTCAAA GTTTTTTTCT TCCATTTCAG 61 GTGTCGTGAG GCTAGCATCG ATTGATCAAC AAGTTTGTAC AAAAAAGTTG GCACCATGCA 121 GGGGGTGGGC AGCCAACCAG AGCTGGTCAC TCCTCAGGAC CATGAGGTAG CTTTGGCCAA 181 TGGATTCCTT TATGAGCATG AAGCATATGG CAATGGCTAT AGCAATGGTC AGCTTGGAAA 241 CCACAGTGAA GAAGACAGCA CTGATGACCA AAGAGAAGAC ACTCATATTA AGCCTATTTA 301 TATGCAATTT CATGCCATTC AGGAATTCTT GCCAACTTTC TTGTACAAAG TGGTTGATAT 361 CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC 421 GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGAGTATAA 481 TACAGCTCGG ATCTAGCCAC GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt 541 gaaagatt