Construct: ORF TRCN0000476458
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000123.1_s317c1
- Derived from:
- BRDN0000391381
- DNA Barcode:
- CAACCAGGCTTTAGGCAGGCCGTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TIAM1 (7074)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000476458
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 7074 | TIAM1 | TIAM Rac1 associated GEF 1 | NM_001353688.1 | 99.8% | 99.7% | (many diffs) |
| 2 | human | 7074 | TIAM1 | TIAM Rac1 associated GEF 1 | NM_001353689.1 | 99.8% | 99.7% | (many diffs) |
| 3 | human | 7074 | TIAM1 | TIAM Rac1 associated GEF 1 | NM_001353690.1 | 99.8% | 99.7% | (many diffs) |
| 4 | human | 7074 | TIAM1 | TIAM Rac1 associated GEF 1 | NM_001353691.1 | 99.8% | 99.7% | (many diffs) |
| 5 | human | 7074 | TIAM1 | TIAM Rac1 associated GEF 1 | NM_001353692.1 | 99.8% | 99.7% | (many diffs) |
| 6 | human | 7074 | TIAM1 | TIAM Rac1 associated GEF 1 | NM_001353693.1 | 99.8% | 99.7% | (many diffs) |
| 7 | human | 7074 | TIAM1 | TIAM Rac1 associated GEF 1 | NM_001353694.1 | 99.8% | 99.7% | (many diffs) |
| 8 | human | 7074 | TIAM1 | TIAM Rac1 associated GEF 1 | NM_003253.3 | 99.8% | 99.7% | (many diffs) |
| 9 | human | 7074 | TIAM1 | TIAM Rac1 associated GEF 1 | XM_005261038.3 | 99.8% | 99.7% | (many diffs) |
| 10 | human | 7074 | TIAM1 | TIAM Rac1 associated GEF 1 | XM_011529712.1 | 99.8% | 99.7% | (many diffs) |
| 11 | human | 7074 | TIAM1 | TIAM Rac1 associated GEF 1 | XM_011529713.1 | 99.8% | 99.7% | (many diffs) |
| 12 | human | 7074 | TIAM1 | TIAM Rac1 associated GEF 1 | XM_017028448.1 | 99.8% | 99.7% | (many diffs) |
| 13 | human | 7074 | TIAM1 | TIAM Rac1 associated GEF 1 | XM_017028450.1 | 99.8% | 99.7% | (many diffs) |
| 14 | human | 7074 | TIAM1 | TIAM Rac1 associated GEF 1 | XM_017028451.2 | 99.8% | 99.7% | (many diffs) |
| 15 | human | 7074 | TIAM1 | TIAM Rac1 associated GEF 1 | XM_024452127.1 | 99.8% | 99.7% | (many diffs) |
| 16 | human | 7074 | TIAM1 | TIAM Rac1 associated GEF 1 | NM_001353686.1 | 98.3% | 98.1% | (many diffs) |
| 17 | human | 7074 | TIAM1 | TIAM Rac1 associated GEF 1 | NM_001353687.1 | 98.3% | 98.1% | (many diffs) |
| 18 | human | 7074 | TIAM1 | TIAM Rac1 associated GEF 1 | XM_005261040.2 | 90.9% | 89.3% | (many diffs) |
| 19 | human | 7074 | TIAM1 | TIAM Rac1 associated GEF 1 | NM_001353684.1 | 38% | 37.3% | (many diffs) |
| 20 | human | 7074 | TIAM1 | TIAM Rac1 associated GEF 1 | NM_001353685.1 | 36.6% | 36% | (many diffs) |
| 21 | mouse | 21844 | Tiam1 | T cell lymphoma invasion an... | NM_001145886.1 | 89.2% | 95.2% | (many diffs) |
| 22 | mouse | 21844 | Tiam1 | T cell lymphoma invasion an... | NM_009384.3 | 89.2% | 95.2% | (many diffs) |
| 23 | mouse | 21844 | Tiam1 | T cell lymphoma invasion an... | XM_006522978.3 | 89.2% | 95.2% | (many diffs) |
| 24 | mouse | 21844 | Tiam1 | T cell lymphoma invasion an... | XM_006522980.3 | 89.2% | 95.2% | (many diffs) |
| 25 | mouse | 21844 | Tiam1 | T cell lymphoma invasion an... | XM_006522982.3 | 89.2% | 95.2% | (many diffs) |
| 26 | mouse | 21844 | Tiam1 | T cell lymphoma invasion an... | XM_011246112.2 | 89.2% | 95.2% | (many diffs) |
| 27 | mouse | 21844 | Tiam1 | T cell lymphoma invasion an... | XM_017316936.1 | 89.2% | 95.2% | (many diffs) |
| 28 | mouse | 21844 | Tiam1 | T cell lymphoma invasion an... | XM_006522983.3 | 71.4% | 77.1% | (many diffs) |
| 29 | mouse | 21844 | Tiam1 | T cell lymphoma invasion an... | NM_001145887.1 | 34.5% | 36% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 4842
- ORF length:
- 4773
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gggaaacgca gaaagtcaag acagagagca cgagttttat ggagaaaagc 121 atgccagcct ggggcgcaag cacacttccc gctccctgcg cctctcgcac aagacgcgga 181 ggaccaggca cgcttcctcg gggaaggtga tccacaggaa ctccgaagtg agcacccgat 241 ccagcagcac ccccagcatc ccccagtccc tggctgaaaa tggcctggag cccttctccc 301 aagatggtac cctagaagac ttcgggagcc ccatctgggt ggaccgagtg gacatgggct 361 tgagacctgt gtcttacact gactcttctg tcactcccag cgtagacagc agcatcgtcc 421 tcacagcagc ctctgtgcag agcatgccag acactgagga gagcaggctt tacggggatg 481 acgctacata tttggctgag ggaggcagga ggcagcattc ctatacatcc aatgggccca 541 ctttcatgga gacggcgagc tttaagaaga aacgctccaa atctgcagac atctggcggg 601 aggacagcct ggaattctca ctctctgatc tgagccaaga acatttaaca agcaacgaag 661 aaatcttggg ttccgccgaa gagaaggact gcgaggaggc tcgggggatg gaaacgcggg 721 cgagtccgcg gcagctcagc acctgtcaga gagccaattc cttgggtgac ttgtatgctc 781 agaaaaactc tggagtgaca gcaaacgggg ggccggggag caaatttgca ggctactgtc 841 ggaatttggt gtctgatatt cccaatcttg caaaccataa gatgccacca gctgctgctg 901 aagagactcc tccgtacagt aattataaca cacttccctg taggaaatct cactgtctct 961 ctgaaggtgc caccaaccca caaattagcc atagcaacag catgcaaggc agaagagcta 1021 aaacaactca ggatgttaat gcaggcgagg gcagtgagtt tgcagacagt gggattgaag 1081 gggccactac cgacacggac ctcctgtcca ggcgatctaa tgccaccaac tccagctact 1141 cacccaccac aggccgggcc tttgtgggca gcgacagcgg cagcagctcc accggggatg 1201 cggctcgtca gggggtgtac gagaacttcc ggcgggagct ggagatgagc accaccaaca 1261 gcgagagcct ggaggaggcc ggctcggcgc acagcgatga gcagagcagc ggcaccctga 1321 gctctccggg ccagtcggac atcctgctga ccgccgcaca gggcacggtg cgcaaggccg 1381 gcgccctggc cgtcaagaac ttcctggtgc acaagaagaa caagaaggtg gagtcagcca 1441 cccggaggaa gtggaagcac tactgggtgt ccctgaaagg atgcacgcta tttttctacg 1501 agagcgacgg caggtctggg atagaccaca acagcatccc caaacacgcc gtctgggtgg 1561 agaacagcat tgtgcaggcg gtgcctgagc accccaagaa ggactttgtc ttctgcctca 1621 gcaattccct gggtgatgcc ttcctttttc agaccactag ccagacggag cttgaaaact 1681 ggatcaccgc catccactct gcctgcgcca ctgcggtcgc gaggcaccac cacaaggaag 1741 acacgctccg actcctgaaa tcagagatca aaaaactgga acagaagatt gacatggatg 1801 aaaagatgaa gaaaatgggt gaaatgcagc tgtcttcagt cactgactca aagaaaaaga 1861 aaacaatatt agatcagatc tttgtctggg agcaaaatct cgagcagttc caaatggacc 1921 tgtttcgttt ccgctgttat ttagccagcc ttcagggtgg ggagctgcca aaccccaaaa 1981 ggcttctcgc ttttgcaagt cgaccaacga aagtggccat gggccgcctt ggaatctttt 2041 cggtatcatc gtttcatgcc ctggtggcag cacgcactgg tgaaactgga gtgagaagac 2101 gtactcaggc catgtccaga tccgcgagca agcgaaggag caggttttct tctctgtggg 2161 gtctggatac tacctccaaa aagaagcagg gacggccaag catcaatcag gtgtttggag 2221 agggaaccga agctgtaaag aaatctttag agggaatatt tgatgacatt gttccagatg 2281 gcaagaggga gaaagaagtg gtcttaccta acgttcacca gcacaaccct gactgcgaca 2341 tttgggtcca cgagtatttc actccatcct ggttctgtct gcccaataat cagcctgccc 2401 tgacggtcgt ccggccaggc gacactgcac gggacaccct ggagctgatt tgcaagacac 2461 atcaactgga tcattctgct cattacctgc gcctgaaatt tctaatagaa aacaaaatgc 2521 agctctatgt tccacagccc gaggaagaca tctatgagct gctgtacaaa gaaattgaaa 2581 tctgtccaaa agtcactcag agcatccaca ttgagaagtc agatacagct gctgatactt 2641 acgggttttc actttcttct gtggaagaag atggtattcg aaggctgtac gtgaatagtg 2701 tgaaggaaac cggtttagct tccaagaaag gcctgaaagc aggagatgag attcttgaga 2761 tcaataatcg tgctgctgac gccctgaact cttctatgct caaagatttc ctctcacagc 2821 cctcgctggg cctcctggtg aggacctacc ccgagctgga ggaaggagtg gagctgctgg 2881 aaagcccgcc ccaccgagtg gacggccctg ccgaccttgg cgagagcccc ctcgcctttc 2941 tcaccagcaa cccagggcac agcctttgca gcgagcaggg cagcagtgct gagaccgctc 3001 cagaggagac cgaggggcca gacttggaat cctcagatga gactgatcac agcagcaaga 3061 gtacagaaca ggtggccgca ttttgccgca gtttgcatga gatgaacccc tctgaccaga 3121 gcccatctcc tcaggactcc acggggcctc agctggcgac catgagacaa ctctcggatg 3181 cagataagct gcgcaaggtg atctgcgagc tcctggagac ggagcgcacc tacgtgaagg 3241 atttaaactg tcttatggag agatacctaa agcctcttca aaaagaaact tttctcaccc 3301 aggatgagct tgacgtgctt tttggaaatt taacggaaat ggtagagttt caagtagaat 3361 tccttaaaac tctagaagat ggagtgagac tggtacctga tttggaaaag cttgagaagg 3421 ttgatcaatt taagaaagtg ctgttctctc tggggggatc attcctgtat tatgctgacc 3481 gcttcaagct ctacagtgcc ttctgcgcca gccacacaaa agttcccaag gtcctggaga 3541 aagtcaagac agacacggct ttcaaggcat tcttggatgc ccagaacccg aagcagcagc 3601 actcatccac gctggagtcg tacctcatca agcccatcca gaggatcctc aagtacccac 3661 ttctgctcag ggagctgttc gccctgaccg atgcggagag cgaggagcac taccacctgg 3721 acgtggccat caagaccatg aacaaggttg ccagtcacat caatgagatg cagaaaatcc 3781 atgaagagtt tggggctgtg tttgaccagc tgattgctga acagactggt gagaaaaaag 3841 aggttgcaga tctgagcatg ggagacctgc ttttgcacac taccgtgatc tggctgaacc 3901 cgccggcctc gctgggcaag tggaaaaagg aaccagagtt ggcagcattc gtcttcaaaa 3961 ctgctgtggt ccttgtgtat aaagatggtt ccaaacagaa gaagaaactt gtaggatctc 4021 acaggctttc catttatgag gactgggacc ccttcagatt tcgacacatg atccccacgg 4081 aagcgctgca ggttcgagct ttggcgagtg cagatgcaga ggcaaatgcc gtgtgtgaaa 4141 ttgtccatgt aaaatccgag tctgaaggga ggccggagag ggtctttcac ttgtgctgca 4201 gctccccaga gagccgaaag gatttcctaa aggctgtgca ttcaatcctg cgtgataagc 4261 acagaagaca gctcctcaaa accgagagcc ttccctcatc ccagcaatat gtcccttttg 4321 gaggcaaaag attgtgtgca ctgaaggggg ccaggccggc catgagcagg gcagtgtctg 4381 ccccaagcaa gtctcttggg aggaggaggc ggcggctggc tcgaaacagg tttaccattg 4441 attctgatgc cgtctccgca agcagcccgg agaaagagtc ccagcagccc cccggtgGTG 4501 GGGACACTGA CCGATGGGTA GAGGAGCAGT TTGATCTTGC TCAGTATGAG GAGCAAGATG 4561 ACATCAAGGA GACAGACATC CTCAGTGACG ATGATGAGTT CTGTGAGTCC GTGAAGGGTG 4621 CCTCAGTGGA CAGAGACCTG CAGGAGCGGC TTCAGGCCAC CTCCATCAGT CAGCGGGAAA 4681 GAGGCCGGAA AACCCTGGAT AGTCACGCGT CCCGCATGGC ACAGCTCAAG AAGCAAGCTG 4741 CCCTGTCGGG GATCAATGGA GGCCTGGAGA GCGCAAGCGA GGAAGTCATT TGGGTTAGGC 4801 GTGAAGACTT TGCCCCCTCC AGGAAACTGA ACACTGAGAT CTTGCCAACT TTCTTGTACA 4861 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 4921 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 4981 AGGACGACAA CCAGGCTTTA GGCAGGCCGT CACGCGTTAA GTCgacaatc aacctctgga 5041 ttacaaaatt tgtgaaagat t