Construct: ORF TRCN0000476458

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF000123.1_s317c1
Derived from:
BRDN0000391381
DNA Barcode:
CAACCAGGCTTTAGGCAGGCCGTC
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
TIAM1 (7074)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000476458
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 7074 TIAM1 TIAM Rac1 associated GEF 1 NM_001353688.1 99.8% 99.7% (many diffs)
2 human 7074 TIAM1 TIAM Rac1 associated GEF 1 NM_001353689.1 99.8% 99.7% (many diffs)
3 human 7074 TIAM1 TIAM Rac1 associated GEF 1 NM_001353690.1 99.8% 99.7% (many diffs)
4 human 7074 TIAM1 TIAM Rac1 associated GEF 1 NM_001353691.1 99.8% 99.7% (many diffs)
5 human 7074 TIAM1 TIAM Rac1 associated GEF 1 NM_001353692.1 99.8% 99.7% (many diffs)
6 human 7074 TIAM1 TIAM Rac1 associated GEF 1 NM_001353693.1 99.8% 99.7% (many diffs)
7 human 7074 TIAM1 TIAM Rac1 associated GEF 1 NM_001353694.1 99.8% 99.7% (many diffs)
8 human 7074 TIAM1 TIAM Rac1 associated GEF 1 NM_003253.3 99.8% 99.7% (many diffs)
9 human 7074 TIAM1 TIAM Rac1 associated GEF 1 XM_005261038.3 99.8% 99.7% (many diffs)
10 human 7074 TIAM1 TIAM Rac1 associated GEF 1 XM_011529712.1 99.8% 99.7% (many diffs)
11 human 7074 TIAM1 TIAM Rac1 associated GEF 1 XM_011529713.1 99.8% 99.7% (many diffs)
12 human 7074 TIAM1 TIAM Rac1 associated GEF 1 XM_017028448.1 99.8% 99.7% (many diffs)
13 human 7074 TIAM1 TIAM Rac1 associated GEF 1 XM_017028450.1 99.8% 99.7% (many diffs)
14 human 7074 TIAM1 TIAM Rac1 associated GEF 1 XM_017028451.2 99.8% 99.7% (many diffs)
15 human 7074 TIAM1 TIAM Rac1 associated GEF 1 XM_024452127.1 99.8% 99.7% (many diffs)
16 human 7074 TIAM1 TIAM Rac1 associated GEF 1 NM_001353686.1 98.3% 98.1% (many diffs)
17 human 7074 TIAM1 TIAM Rac1 associated GEF 1 NM_001353687.1 98.3% 98.1% (many diffs)
18 human 7074 TIAM1 TIAM Rac1 associated GEF 1 XM_005261040.2 90.9% 89.3% (many diffs)
19 human 7074 TIAM1 TIAM Rac1 associated GEF 1 NM_001353684.1 38% 37.3% (many diffs)
20 human 7074 TIAM1 TIAM Rac1 associated GEF 1 NM_001353685.1 36.6% 36% (many diffs)
21 mouse 21844 Tiam1 T cell lymphoma invasion an... NM_001145886.1 89.2% 95.2% (many diffs)
22 mouse 21844 Tiam1 T cell lymphoma invasion an... NM_009384.3 89.2% 95.2% (many diffs)
23 mouse 21844 Tiam1 T cell lymphoma invasion an... XM_006522978.3 89.2% 95.2% (many diffs)
24 mouse 21844 Tiam1 T cell lymphoma invasion an... XM_006522980.3 89.2% 95.2% (many diffs)
25 mouse 21844 Tiam1 T cell lymphoma invasion an... XM_006522982.3 89.2% 95.2% (many diffs)
26 mouse 21844 Tiam1 T cell lymphoma invasion an... XM_011246112.2 89.2% 95.2% (many diffs)
27 mouse 21844 Tiam1 T cell lymphoma invasion an... XM_017316936.1 89.2% 95.2% (many diffs)
28 mouse 21844 Tiam1 T cell lymphoma invasion an... XM_006522983.3 71.4% 77.1% (many diffs)
29 mouse 21844 Tiam1 T cell lymphoma invasion an... NM_001145887.1 34.5% 36% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
4842
ORF length:
4773
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcaccat gggaaacgca gaaagtcaag acagagagca cgagttttat ggagaaaagc
121atgccagcct ggggcgcaag cacacttccc gctccctgcg cctctcgcac aagacgcgga
181ggaccaggca cgcttcctcg gggaaggtga tccacaggaa ctccgaagtg agcacccgat
241ccagcagcac ccccagcatc ccccagtccc tggctgaaaa tggcctggag cccttctccc
301aagatggtac cctagaagac ttcgggagcc ccatctgggt ggaccgagtg gacatgggct
361tgagacctgt gtcttacact gactcttctg tcactcccag cgtagacagc agcatcgtcc
421tcacagcagc ctctgtgcag agcatgccag acactgagga gagcaggctt tacggggatg
481acgctacata tttggctgag ggaggcagga ggcagcattc ctatacatcc aatgggccca
541ctttcatgga gacggcgagc tttaagaaga aacgctccaa atctgcagac atctggcggg
601aggacagcct ggaattctca ctctctgatc tgagccaaga acatttaaca agcaacgaag
661aaatcttggg ttccgccgaa gagaaggact gcgaggaggc tcgggggatg gaaacgcggg
721cgagtccgcg gcagctcagc acctgtcaga gagccaattc cttgggtgac ttgtatgctc
781agaaaaactc tggagtgaca gcaaacgggg ggccggggag caaatttgca ggctactgtc
841ggaatttggt gtctgatatt cccaatcttg caaaccataa gatgccacca gctgctgctg
901aagagactcc tccgtacagt aattataaca cacttccctg taggaaatct cactgtctct
961ctgaaggtgc caccaaccca caaattagcc atagcaacag catgcaaggc agaagagcta
1021aaacaactca ggatgttaat gcaggcgagg gcagtgagtt tgcagacagt gggattgaag
1081gggccactac cgacacggac ctcctgtcca ggcgatctaa tgccaccaac tccagctact
1141cacccaccac aggccgggcc tttgtgggca gcgacagcgg cagcagctcc accggggatg
1201cggctcgtca gggggtgtac gagaacttcc ggcgggagct ggagatgagc accaccaaca
1261gcgagagcct ggaggaggcc ggctcggcgc acagcgatga gcagagcagc ggcaccctga
1321gctctccggg ccagtcggac atcctgctga ccgccgcaca gggcacggtg cgcaaggccg
1381gcgccctggc cgtcaagaac ttcctggtgc acaagaagaa caagaaggtg gagtcagcca
1441cccggaggaa gtggaagcac tactgggtgt ccctgaaagg atgcacgcta tttttctacg
1501agagcgacgg caggtctggg atagaccaca acagcatccc caaacacgcc gtctgggtgg
1561agaacagcat tgtgcaggcg gtgcctgagc accccaagaa ggactttgtc ttctgcctca
1621gcaattccct gggtgatgcc ttcctttttc agaccactag ccagacggag cttgaaaact
1681ggatcaccgc catccactct gcctgcgcca ctgcggtcgc gaggcaccac cacaaggaag
1741acacgctccg actcctgaaa tcagagatca aaaaactgga acagaagatt gacatggatg
1801aaaagatgaa gaaaatgggt gaaatgcagc tgtcttcagt cactgactca aagaaaaaga
1861aaacaatatt agatcagatc tttgtctggg agcaaaatct cgagcagttc caaatggacc
1921tgtttcgttt ccgctgttat ttagccagcc ttcagggtgg ggagctgcca aaccccaaaa
1981ggcttctcgc ttttgcaagt cgaccaacga aagtggccat gggccgcctt ggaatctttt
2041cggtatcatc gtttcatgcc ctggtggcag cacgcactgg tgaaactgga gtgagaagac
2101gtactcaggc catgtccaga tccgcgagca agcgaaggag caggttttct tctctgtggg
2161gtctggatac tacctccaaa aagaagcagg gacggccaag catcaatcag gtgtttggag
2221agggaaccga agctgtaaag aaatctttag agggaatatt tgatgacatt gttccagatg
2281gcaagaggga gaaagaagtg gtcttaccta acgttcacca gcacaaccct gactgcgaca
2341tttgggtcca cgagtatttc actccatcct ggttctgtct gcccaataat cagcctgccc
2401tgacggtcgt ccggccaggc gacactgcac gggacaccct ggagctgatt tgcaagacac
2461atcaactgga tcattctgct cattacctgc gcctgaaatt tctaatagaa aacaaaatgc
2521agctctatgt tccacagccc gaggaagaca tctatgagct gctgtacaaa gaaattgaaa
2581tctgtccaaa agtcactcag agcatccaca ttgagaagtc agatacagct gctgatactt
2641acgggttttc actttcttct gtggaagaag atggtattcg aaggctgtac gtgaatagtg
2701tgaaggaaac cggtttagct tccaagaaag gcctgaaagc aggagatgag attcttgaga
2761tcaataatcg tgctgctgac gccctgaact cttctatgct caaagatttc ctctcacagc
2821cctcgctggg cctcctggtg aggacctacc ccgagctgga ggaaggagtg gagctgctgg
2881aaagcccgcc ccaccgagtg gacggccctg ccgaccttgg cgagagcccc ctcgcctttc
2941tcaccagcaa cccagggcac agcctttgca gcgagcaggg cagcagtgct gagaccgctc
3001cagaggagac cgaggggcca gacttggaat cctcagatga gactgatcac agcagcaaga
3061gtacagaaca ggtggccgca ttttgccgca gtttgcatga gatgaacccc tctgaccaga
3121gcccatctcc tcaggactcc acggggcctc agctggcgac catgagacaa ctctcggatg
3181cagataagct gcgcaaggtg atctgcgagc tcctggagac ggagcgcacc tacgtgaagg
3241atttaaactg tcttatggag agatacctaa agcctcttca aaaagaaact tttctcaccc
3301aggatgagct tgacgtgctt tttggaaatt taacggaaat ggtagagttt caagtagaat
3361tccttaaaac tctagaagat ggagtgagac tggtacctga tttggaaaag cttgagaagg
3421ttgatcaatt taagaaagtg ctgttctctc tggggggatc attcctgtat tatgctgacc
3481gcttcaagct ctacagtgcc ttctgcgcca gccacacaaa agttcccaag gtcctggaga
3541aagtcaagac agacacggct ttcaaggcat tcttggatgc ccagaacccg aagcagcagc
3601actcatccac gctggagtcg tacctcatca agcccatcca gaggatcctc aagtacccac
3661ttctgctcag ggagctgttc gccctgaccg atgcggagag cgaggagcac taccacctgg
3721acgtggccat caagaccatg aacaaggttg ccagtcacat caatgagatg cagaaaatcc
3781atgaagagtt tggggctgtg tttgaccagc tgattgctga acagactggt gagaaaaaag
3841aggttgcaga tctgagcatg ggagacctgc ttttgcacac taccgtgatc tggctgaacc
3901cgccggcctc gctgggcaag tggaaaaagg aaccagagtt ggcagcattc gtcttcaaaa
3961ctgctgtggt ccttgtgtat aaagatggtt ccaaacagaa gaagaaactt gtaggatctc
4021acaggctttc catttatgag gactgggacc ccttcagatt tcgacacatg atccccacgg
4081aagcgctgca ggttcgagct ttggcgagtg cagatgcaga ggcaaatgcc gtgtgtgaaa
4141ttgtccatgt aaaatccgag tctgaaggga ggccggagag ggtctttcac ttgtgctgca
4201gctccccaga gagccgaaag gatttcctaa aggctgtgca ttcaatcctg cgtgataagc
4261acagaagaca gctcctcaaa accgagagcc ttccctcatc ccagcaatat gtcccttttg
4321gaggcaaaag attgtgtgca ctgaaggggg ccaggccggc catgagcagg gcagtgtctg
4381ccccaagcaa gtctcttggg aggaggaggc ggcggctggc tcgaaacagg tttaccattg
4441attctgatgc cgtctccgca agcagcccgg agaaagagtc ccagcagccc cccggtgGTG
4501GGGACACTGA CCGATGGGTA GAGGAGCAGT TTGATCTTGC TCAGTATGAG GAGCAAGATG
4561ACATCAAGGA GACAGACATC CTCAGTGACG ATGATGAGTT CTGTGAGTCC GTGAAGGGTG
4621CCTCAGTGGA CAGAGACCTG CAGGAGCGGC TTCAGGCCAC CTCCATCAGT CAGCGGGAAA
4681GAGGCCGGAA AACCCTGGAT AGTCACGCGT CCCGCATGGC ACAGCTCAAG AAGCAAGCTG
4741CCCTGTCGGG GATCAATGGA GGCCTGGAGA GCGCAAGCGA GGAAGTCATT TGGGTTAGGC
4801GTGAAGACTT TGCCCCCTCC AGGAAACTGA ACACTGAGAT CTTGCCAACT TTCTTGTACA
4861AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT
4921AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA
4981AGGACGACAA CCAGGCTTTA GGCAGGCCGT CACGCGTTAA GTCgacaatc aacctctgga
5041ttacaaaatt tgtgaaagat t

Download FASTA (ORF) (Full)