Construct: ORF TRCN0000476597

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF014303.1_s317c1
Derived from:
ccsbBroadEn_00202
DNA Barcode:
TATGCGGCAACACTGTTGCCTGAA
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
CACNB4 (785)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000476597
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 785 CACNB4 calcium voltage-gated chann... NM_000726.4 100% 100%
2 human 785 CACNB4 calcium voltage-gated chann... NM_001005746.4 95.6% 95.9% 0_1ins61;2_8delTGGATGT
3 human 785 CACNB4 calcium voltage-gated chann... NM_001005747.4 91.4% 89.7% (many diffs)
4 human 785 CACNB4 calcium voltage-gated chann... NM_001320722.3 90.8% 90.5% 0_1ins109;6_6delTins33
5 human 785 CACNB4 calcium voltage-gated chann... NM_001330118.1 90.8% 90.5% 0_1ins109;6_6delTins33
6 human 785 CACNB4 calcium voltage-gated chann... XM_011511796.2 90.7% 90.5% 0_1ins135;2_3insCCTCAGACA
7 human 785 CACNB4 calcium voltage-gated chann... NM_001145798.2 88% 88% 1116_1117ins186
8 human 785 CACNB4 calcium voltage-gated chann... XM_011511797.3 87.8% 84.2% 597_598insGCAAAACAGAAGCAAAAAGT;677_678ins169
9 human 785 CACNB4 calcium voltage-gated chann... NM_001330113.2 83.5% 80.2% (many diffs)
10 human 785 CACNB4 calcium voltage-gated chann... NM_001330115.2 79.4% 74.1% (many diffs)
11 human 785 CACNB4 calcium voltage-gated chann... NM_001330116.2 78.7% 74.8% (many diffs)
12 human 785 CACNB4 calcium voltage-gated chann... NM_001330117.2 64.2% 64.2% 0_1ins558
13 human 785 CACNB4 calcium voltage-gated chann... XM_024453128.1 64.2% 64.2% 0_1ins558
14 human 785 CACNB4 calcium voltage-gated chann... NM_001330114.2 58% 58% 0_1ins654
15 human 785 CACNB4 calcium voltage-gated chann... XM_017004885.1 58% 58% 0_1ins654
16 human 785 CACNB4 calcium voltage-gated chann... XR_923022.3 47.6% 1_153del;1454_1525del;1786_3271del
17 human 785 CACNB4 calcium voltage-gated chann... XR_002959337.1 45.4% 1_153del;1454_1688del;1949_3434del
18 human 785 CACNB4 calcium voltage-gated chann... XR_002959338.1 44.2% (many diffs)
19 human 785 CACNB4 calcium voltage-gated chann... XM_017004888.2 40.5% 33.8% (many diffs)
20 human 785 CACNB4 calcium voltage-gated chann... XR_001738938.2 39.2% (many diffs)
21 human 785 CACNB4 calcium voltage-gated chann... XR_001738928.1 30.6% (many diffs)
22 human 785 CACNB4 calcium voltage-gated chann... XR_001738939.1 17.9% (many diffs)
23 human 785 CACNB4 calcium voltage-gated chann... XR_001738940.2 17.6% (many diffs)
24 human 785 CACNB4 calcium voltage-gated chann... XR_001738935.1 16.5% (many diffs)
25 human 785 CACNB4 calcium voltage-gated chann... XR_001738937.2 16.3% (many diffs)
26 mouse 12298 Cacnb4 calcium channel, voltage-de... NM_001037099.2 89.9% 98.2% (many diffs)
27 mouse 12298 Cacnb4 calcium channel, voltage-de... NM_001285426.1 86.2% 95.1% (many diffs)
28 mouse 12298 Cacnb4 calcium channel, voltage-de... NM_146123.3 82.6% 88.9% (many diffs)
29 mouse 12298 Cacnb4 calcium channel, voltage-de... NM_001285427.1 81.6% 89.8% (many diffs)
30 mouse 12298 Cacnb4 calcium channel, voltage-de... XM_006497641.3 81.6% 89.8% (many diffs)
31 mouse 12298 Cacnb4 calcium channel, voltage-de... XM_011239015.1 81.6% 89.8% (many diffs)
32 mouse 12298 Cacnb4 calcium channel, voltage-de... XM_017315163.1 81.6% 89.8% (many diffs)
33 mouse 12298 Cacnb4 calcium channel, voltage-de... XM_006497642.3 63.6% 67.3% (many diffs)
34 mouse 12298 Cacnb4 calcium channel, voltage-de... XM_017315165.1 63.6% 67.3% (many diffs)
35 mouse 12298 Cacnb4 calcium channel, voltage-de... NM_001285428.1 58.1% 63.4% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
1629
ORF length:
1560
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcaccat gtcctcctcc tcctacgcca agaacgggac cgcggacggg ccgcactccc
121ccacctcgca ggtggcccga ggcaccacaa cccggaggag caggttgaaa agatccgatg
181gcagcaccac ttcgaccagc ttcatcctca gacagggttc agcggattcc tacacaagca
241ggccgtctga ctccgatgtc tctttggaag aggaccggga agcaattcga caggagagag
301aacagcaagc agctatccag cttgagagag caaagtccaa acctgtagca tttgccgtga
361agacaaatgt gagctactgc ggcgccctgg acgaggatgt gcctgttcca agcacagcta
421tctcctttga tgctaaagac tttctacata ttaaagagaa atataacaat gattggtgga
481taggaaggct ggtgaaagag ggctgtgaaa ttggcttcat tccaagtcca ctcagattgg
541agaacatacg gatccagcaa gaacaaaaaa gaggacgttt tcacggaggg aaatcaagtg
601gaaattcttc ttcaagtctt ggagaaatgg tatctgggac attccgagca actcccacat
661caacagcaaa acagaagcaa aaagtgacgg agcacattcc tccttacgat gttgtaccgt
721caatgcgtcc ggtggtgtta gtggggccgt cactgaaagg ttacgaggta acagacatga
781tgcagaaagc cctctttgat ttcctgaagc acaggtttga tgggaggatt tcaataacga
841gagtgacagc tgacatttct cttgctaaga ggtctgtcct aaataatccc agcaagagag
901caataattga acgttcgaac acccggtcca gcttagcgga agtacaaagt gaaattgaaa
961gaatctttga gttggcaaga tctttgcaac tggttgttct tgatgcagac accatcaatc
1021acccagcaca acttataaag acttccttag caccaattat tgttcatgta aaagtctcat
1081ctccaaaggt tttacagcgg ttgattaaat ctagaggaaa gtcacaaagt aaacacttga
1141atgttcaact ggtggcagct gataaacttg cacaatgccc cccagaaatg tttgatgtta
1201tattggatga aaatcagctt gaggatgcat gtgaacatct aggggagtac ctGGAGGCGT
1261ACTGGCGTGC CACCCACACA ACCAGTAGCA CACCCATGAC CCCGCTGCTG GGAAGGAATT
1321TGGGCTCCAC GGCACTCTCA CCATATCCCA CAGCAATTTC TGGGTTACAG AGTCAGCGAA
1381TGAGGCACAG CAACCACTCC ACAGAGAACT CTCCAATTGA AAGACGAAGT CTAATGACCT
1441CTGATGAAAA TTATCACAAT GAAAGGGCTC GGAAGAGTAG GAACCGCTTG TCTTCCAGTT
1501CTCAGCATAG CCGAGATCAT TACCCTCTTG TGGAAGAAGA TTACCCTGAC TCATACCAGG
1561ACACTTACAA ACCCCATAGG AACCGAGGAT CACCTGGGGG ATATAGCCAT GACTCCCGAC
1621ATAGGCTTTT GCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC
1681CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA
1741TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGATATGCG GCAACACTGT TGCCTGAAAC
1801GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt

Download FASTA (ORF) (Full)