Construct: ORF TRCN0000476672
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003384.1_s317c1
- Derived from:
- ccsbBroadEn_07875
- DNA Barcode:
- AGTGTACCACCTCGAAGCACATGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SPECC1L (23384)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000476672
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 23384 | SPECC1L | sperm antigen with calponin... | NM_001145468.3 | 100% | 100% | |
| 2 | human | 23384 | SPECC1L | sperm antigen with calponin... | NM_015330.5 | 100% | 100% | |
| 3 | human | 23384 | SPECC1L | sperm antigen with calponin... | NM_001254732.2 | 96.5% | 96.5% | 3085_3086ins117 |
| 4 | human | 101730217 | SPECC1L-ADORA2A | SPECC1L-ADORA2A readthrough... | NR_103546.1 | 53.2% | 1_308del;2958_3079del;3782_6289del | |
| 5 | human | 23384 | SPECC1L | sperm antigen with calponin... | NM_001254733.1 | 16.3% | 16.3% | 0_1ins2802 |
| 6 | mouse | 74392 | Specc1l | sperm antigen with calponin... | NM_153406.3 | 88.3% | 95.2% | (many diffs) |
| 7 | mouse | 74392 | Specc1l | sperm antigen with calponin... | XM_006514252.3 | 88.3% | 95.2% | (many diffs) |
| 8 | mouse | 74392 | Specc1l | sperm antigen with calponin... | XM_017314127.1 | 88.3% | 95.2% | (many diffs) |
| 9 | mouse | 74392 | Specc1l | sperm antigen with calponin... | NM_001145826.1 | 87% | 93.8% | (many diffs) |
| 10 | mouse | 74392 | Specc1l | sperm antigen with calponin... | XM_011243589.2 | 87% | 93.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 3420
- ORF length:
- 3351
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gaagaaagca agcaggagtg ttggctcagt gcctaaagtg tctgcaataa 121 gtaaaacgca aacagcagaa aaaattaaac ctgaaaacag ctcttcagca tctacgggag 181 gcaaacttgt aaaacctgga acagcagcat cattgtcaaa gaccaagagc agtgatgacc 241 ttttagctgg aatggccgga ggggtaacgg tgactaatgg tgttaaagga aagaaaagca 301 cctgcccatc tgcagcacct tcagcatctg cccctgccat gaccaccgtg gagaacaaat 361 ccaagattag cacaggcaca gcttcttcaa ccaagcggag cacttctaca ggtaataaag 421 aatccagttc tactagagaa agattacgtg aacgtacccg attaaaccag agcaaaaaac 481 taccttctgc aggtcaggga gctaatgaca tggcattggc caaacgttcc cgcagtcgaa 541 ctgctacaga atgtgacgtt cgtatgagca agtctaagtc agacaatcag atcagtgaca 601 gagctgcttt ggaggccaaa gtgaaggatc ttctcacgct ggcaaaaacc aaagacgtag 661 aaattttaca tttgagaaat gaactgcgag acatgcgtgc ccagctgggc attaatgagg 721 atcattctga gggtgatgaa aaatctgaga aggaaactat tatggctcac cagccgactg 781 atgtggagtc cactttattg cagttgcagg aacagaatac tgccatccgt gaagaactca 841 accagctgaa aaatgaaaac agaatgttaa aggacaggtt gaatgcattg ggcttttccc 901 tagagcagag gttagacaat tctgaaaaac tgtttggcta tcagtccctg agcccagaaa 961 tcacccctgg taaccagagc gatggaggag gaactctgac ttcttcagtg gaaggctctg 1021 cccctggctc agtggaggat ctcttgagtc aggatgaaaa tacactaatg gaccatcagc 1081 acagtaactc catggacaat ttagacagtg agtgcagtga ggtctaccag cccctcacat 1141 cgagcgatga tgcgctggat gcaccatcct cctcagagtc ggaaggcatc cccagcatag 1201 agcgctcccg gaaggggagc agcgggaatg ccagtgaagt gtccgtggct tgcctgactg 1261 aacggataca ccagatggaa gagaaccaac acagtacaag tgaggaactc caggcaaccc 1321 tgcaagagct agctgattta cagcagatta cccaggaact gaatagtgaa aacgaaaggc 1381 ttggagaaga gaaggttatt ctgatggagt ctttatgtca gcagagcgat aagttggaac 1441 actttagtcg acagattgaa tacttccgct ctcttctaga tgagcatcac atttcttatg 1501 tcatagatga agatgtaaaa agtgggcgct atatggaatt agagcaacgt tacatggacc 1561 tcgctgagaa tgcccgtttt gaacgggagc agcttcttgg tgtccagcag catttaagca 1621 atactttgaa aatggcagaa caagacaata aggaagctca agaaatgata ggggcactca 1681 aagaacgcag tcaccatatg gagcgaatta ttgagtctga gcagaaagga aaagcagcct 1741 tggcagccac gttagaggaa tacaaagcca cagtggccag tgaccagata gagatgaatc 1801 gcctgaaggc tcagctggag aatgaaaagc agaaagtggc agagctgtat tctatccata 1861 actctggaga caaatctgat attcaggacc tcctggagag tgtcaggctg gacaaagaaa 1921 aagcagagac tttggctagt agcttgcagg aagatctggc tcatacccga aatgatgcca 1981 atcgattaca ggatgccatt gctaaggtag aggatgaata ccgagccttc caagaagaag 2041 ctaagaaaca aattgaagat ttgaatatga cgttagaaaa attaagatca gacctggatg 2101 aaaaagaaac agaaaggagt gacatgaaag aaaccatctt tgaacttgaa gatgaagtag 2161 aacaacatcg tgctgtgaaa cttcatgaca acctcattat ttctgatcta gagaatacag 2221 ttaaaaaact ccaggaccaa aagcacgaca tggaaagaga aataaagaca ctccacagaa 2281 gacttcggga agaatctgcg gaatggcggc agtttcaggc tgatctccag actgcagtag 2341 tcattgcaaa tgacattaaa tctgaagccc aagaggagat tggtgatcta aagcgccggt 2401 tacatgaggc tcaagaaaaa aatgagaaac tcacaaaaga attggaggaa ataaagtcac 2461 gcaagcaaga ggaggagcga ggccgggtat acaattacat gaatgccgtt gagagagatt 2521 tggcagcctt aaggcaggga atgggactga gtagaaggtc ctcgacttcc tcagagccaa 2581 ctcctacagt aaaaaccctc atcaagtcct ttgacagtgc atctcaagta ccaaaccctg 2641 ctgcagctgc aattcctcga acgcccctga gcccaagtcc tatgaaaacc cctcctgcag 2701 cagctgtgtc ccctatgcag agacattcca taagtggacc aatctcaaca tccaaacccc 2761 tgacagccct gtcagataag agaccaaact atggggaaat ccctgttcaa gagcatctgt 2821 taagaacatc ttcagccagc cggcctgctt ccctgccaag agtgcctgcg atggaaagtg 2881 ccaagaccct ctcagtgtct cgacgaagta gtgaagaaat gaaacgggac atttctgcac 2941 aggagggagc gtcgccagcc tctctgatgg ctatgggaac cacgtctcca cagctttccc 3001 tgtccTCTTC TCCAACGGCA TCTGTGACTC CCACCACCCG AAGCCGAATA AGAGAAGAAA 3061 GGAAAGACCC TCTCTCAGCA TTGGCCAGAG AATATGGAGG ATCAAAGAGG AACGCCTTGC 3121 TGAAGTGGTG TCAGAAGAAA ACAGAAGGCT ATCAGAATAT TGACATTACA AACTTCAGCA 3181 GCAGCTGGAA TGATGGGCTG GCCTTCTGTG CCCTCCTGCA TACATATCTC CCTGCCCACA 3241 TTCCATATCA AGAACTGAAC AGCCAGGATA AGAGAAGGAA CTTCATGCTG GCTTTCCAGG 3301 CAGCTGAAAG TGTCGGCATC AAATCCACAC TGGACATTAA TGAAATGGTA CGGACTGAAC 3361 GACCCGACTG GCAGAACGTG ATGCTGTATG TGACGGCGAT CTACAAGTAC TTTGAGACCT 3421 TGCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 3481 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 3541 TTTATATATC TTGTGGAAAG GACGAAGTGT ACCACCTCGA AGCACATGTA CGCGTTAAGT 3601 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt