Construct: ORF TRCN0000476735
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016257.1_s317c1
- Derived from:
- ccsbBroadEn_04855
- DNA Barcode:
- TTCGGATTATGATGCACGAGTGCT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- FBLN7 (129804)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000476735
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 129804 | FBLN7 | fibulin 7 | NM_001128165.1 | 100% | 100% | |
| 2 | human | 129804 | FBLN7 | fibulin 7 | NM_153214.3 | 89.5% | 89.5% | 532_669del |
| 3 | human | 129804 | FBLN7 | fibulin 7 | XM_011510585.2 | 86.1% | 84.5% | (many diffs) |
| 4 | human | 129804 | FBLN7 | fibulin 7 | XM_024452686.1 | 76.5% | 76.5% | 0_1ins171;361_498del |
| 5 | human | 129804 | FBLN7 | fibulin 7 | XM_011510587.2 | 73.6% | 72% | (many diffs) |
| 6 | human | 129804 | FBLN7 | fibulin 7 | XM_017003317.1 | 66.5% | 60.3% | (many diffs) |
| 7 | human | 129804 | FBLN7 | fibulin 7 | XM_006712260.3 | 62.1% | 61.5% | (many diffs) |
| 8 | human | 129804 | FBLN7 | fibulin 7 | XM_017003319.1 | 61.7% | 61.2% | 532_669del;946_947insTCCAGTG;951_952ins359 |
| 9 | human | 129804 | FBLN7 | fibulin 7 | XR_001738616.1 | 59.6% | (many diffs) | |
| 10 | human | 129804 | FBLN7 | fibulin 7 | XR_001738615.1 | 59% | (many diffs) | |
| 11 | human | 129804 | FBLN7 | fibulin 7 | XR_001738614.2 | 58.3% | (many diffs) | |
| 12 | human | 129804 | FBLN7 | fibulin 7 | XR_001738613.1 | 50.7% | (many diffs) | |
| 13 | human | 129804 | FBLN7 | fibulin 7 | XR_001738617.1 | 47.9% | (many diffs) | |
| 14 | human | 129804 | FBLN7 | fibulin 7 | XR_001738618.2 | 25% | (many diffs) | |
| 15 | mouse | 70370 | Fbln7 | fibulin 7 | NM_024237.4 | 77.1% | 76.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1248
- ORF length:
- 1179
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggtgcccagc tctccgcgcg cgctcttcct tctgctcctg atcctcgcct 121 gccccgagcc gcgggcttcc cagaactgtc tcagcaaaca gcagctcctc tcggccatcc 181 gccagctgca gcagctgctg aagggccagg agacacgctt cgccgagggc atccgccaca 241 tgaagagccg gctggccgcg ctgcagaact ctgtgggcag ggtgggccca gatgcccttc 301 cagtttcctg cccggctctg aacacccccg cagacggcag aaagtttgga agcaagtact 361 tagtggatca cgaagtccat tttacctgca accctgggtt ccggctggtc gggcccagca 421 gcgtggtgtg tcttcccaat ggcacctgga caggggagca gccccactgt agaggtatca 481 gtgaatgctc cagccagcct tgtcaaaatg gtggtacatg tgtagaagga gtcaaccagt 541 acagatgcat ttgtcctcca ggaaggactg ggaaccgctg tcagcatcag gcccagactg 601 acgtgaacga gtgtgagctc tacgggcagg aggggcgccc ccggctctgc atgcacgcct 661 gcgtgaacac cccgggctct taccgttgca cctgccccgg tggataccga actctggctg 721 acgggaagag ctgtgaggat gtggatgaat gtgtgggcct gcagccggtg tgcccccagg 781 ggaccacatg catcaacacc ggtggaagct tccagtgtgt cagcccTGAG TGCCCCGAGG 841 GCAGCGGCAA TGTGAGCTAC GTGAAGACGT CTCCATTCCA GTGTGAGCGG AACCCCTGCC 901 CCATGGACAG CAGGCCCTGC CGCCATCTGC CCAAGACCAT CTCCTTCCAT TACCTCTCTC 961 TGCCTTCCAA CCTGAAGACG CCCATCACGC TCTTCCGCAT GGCCACAGCC TCTGCCCCCG 1021 GCCGAGCTGG GCCCAACAGC CTGCGGTTTG GGATCGTGGG TGGGAACAGC CGCGGCCACT 1081 TTGTGATGCA GCGTTCAGAC CGGCAGACTG GGGATCTGAT CCTTGTGCAG AACCTGGAGG 1141 GGCCTCAGAC GCTGGAGGTG GACGTCGACA TGTCGGAATA CCTGGACCGC TCCTTCCAGG 1201 CCAACCACGT GTCCAAGGTC ACCATCTTTG TATCCCCCTA TGACTTCTTG CCAACTTTCT 1261 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 1321 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 1381 GTGGAAAGGA CGATTCGGAT TATGATGCAC GAGTGCTACG CGTTAAGTCg acaatcaacc 1441 tctggattac aaaatttgtg aaagatt