Transcript: Human XM_017003317.1

PREDICTED: Homo sapiens fibulin 7 (FBLN7), transcript variant X9, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
FBLN7 (129804)
Length:
1766
CDS:
272..1327

Additional Resources:

NCBI RefSeq record:
XM_017003317.1
NBCI Gene record:
FBLN7 (129804)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_017003317.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000053944 CGGCAATGTGAGCTACGTGAA pLKO.1 1186 CDS 100% 4.050 5.670 N FBLN7 n/a
2 TRCN0000174242 CGGCAATGTGAGCTACGTGAA pLKO.1 1186 CDS 100% 4.050 5.670 N FBLN7 n/a
3 TRCN0000416441 AGTTTGGAAGCAAGTACTTAG pLKO_005 546 CDS 100% 10.800 7.560 N Fbln7 n/a
4 TRCN0000053947 TCCCAGAACTGTCTCAGCAAA pLKO.1 341 CDS 100% 4.950 3.465 N FBLN7 n/a
5 TRCN0000053943 CGGCAGAAAGTTTGGAAGCAA pLKO.1 538 CDS 100% 3.000 2.100 N FBLN7 n/a
6 TRCN0000053945 GATGCATTTGTCCTCCAGGAA pLKO.1 747 CDS 100% 2.640 1.848 N FBLN7 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_017003317.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_04855 pDONR223 100% 66.5% 60.3% None (many diffs) n/a
2 ccsbBroad304_04855 pLX_304 0% 66.5% 60.3% V5 (many diffs) n/a
3 TRCN0000476735 TTCGGATTATGATGCACGAGTGCT pLX_317 35.9% 66.5% 60.3% V5 (many diffs) n/a
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