Construct: ORF TRCN0000476740
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017326.1_s317c1
- Derived from:
- ccsbBroadEn_03401
- DNA Barcode:
- CGTTTTACCATCTAGCTCGATGCT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- YBEY (54059)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000476740
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 54059 | YBEY | ybeY metalloendoribonuclease | NM_001006114.2 | 100% | 100% | |
| 2 | human | 54059 | YBEY | ybeY metalloendoribonuclease | NM_001314026.1 | 100% | 100% | |
| 3 | human | 54059 | YBEY | ybeY metalloendoribonuclease | XM_017028396.1 | 100% | 100% | |
| 4 | human | 54059 | YBEY | ybeY metalloendoribonuclease | NM_001314025.2 | 71.3% | 68.2% | (many diffs) |
| 5 | human | 54059 | YBEY | ybeY metalloendoribonuclease | NM_058181.3 | 71.3% | 68.2% | (many diffs) |
| 6 | human | 54059 | YBEY | ybeY metalloendoribonuclease | NM_001314022.2 | 66.3% | 51.6% | (many diffs) |
| 7 | human | 54059 | YBEY | ybeY metalloendoribonuclease | XM_011529633.2 | 64.7% | 61.9% | (many diffs) |
| 8 | human | 54059 | YBEY | ybeY metalloendoribonuclease | XM_011529631.2 | 57.8% | 55.3% | (many diffs) |
| 9 | human | 54059 | YBEY | ybeY metalloendoribonuclease | XM_011529629.2 | 55.1% | 52.7% | (many diffs) |
| 10 | human | 54059 | YBEY | ybeY metalloendoribonuclease | XM_011529630.2 | 55.1% | 52.7% | (many diffs) |
| 11 | human | 54059 | YBEY | ybeY metalloendoribonuclease | XM_011529635.2 | 55.1% | 52.7% | (many diffs) |
| 12 | human | 54059 | YBEY | ybeY metalloendoribonuclease | NM_001314023.2 | 44.4% | 41.3% | (many diffs) |
| 13 | human | 54059 | YBEY | ybeY metalloendoribonuclease | XM_017028394.1 | 36.1% | 24.4% | (many diffs) |
| 14 | human | 54059 | YBEY | ybeY metalloendoribonuclease | XM_005261156.4 | 34.9% | 27.8% | (many diffs) |
| 15 | human | 54059 | YBEY | ybeY metalloendoribonuclease | XM_011529634.2 | 30.9% | 24.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 93
- ORF end:
- 456
- ORF length:
- 363
- Sequence:
-
1 TCAGACAGTG GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGCT AGCATCGATT 61 GATCAACAAG TTTGTACAAA AAAGTTGGCA CCATGAGTTT GGTGATTAGA AATCTGCAGC 121 GAGTCATCCC CATCAGGAGA GCGCCACTTC GCAGTAAGAT CGAGATTGTA AGGAGGATTT 181 TAGGAGTGCA GAAATTTGAC CTGGGGATCA TCTGTGTTGA CAACAAGAAT ATTCAGCACA 241 TTAATAGAAT CTACAGAGAT AGAAATGTCC CAACCGATGT GCTTTCTTTT CCATTTCATG 301 AGCATCTGAA AGCAGGTGAA TTTCCCCAGC CTGATTTTCC AGATGACTAC AATTTGGGAG 361 ACATTTTCCT AGGAGTGGAG TATATCTTCC ATCAGTGTAA AGAAAATGAA GATTACAATG 421 ACGTCCTGAC TGTAAGCGGG GATGCTGAAA TCATATTGCC AACTTTCTTG TACAAAGTGG 481 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 541 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 601 ACGTTTTACC ATCTAGCTCG ATGCTACGCG TTAAGTCgac aatcaacctc tggattacaa 661 aatttgtgaa agatt