Construct: ORF TRCN0000477049
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000304.1_s317c1
- Derived from:
- ccsbBroadEn_07328
- DNA Barcode:
- GATGGTCCCCGTGCCAATGTATCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PRPF4B (8899)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477049
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8899 | PRPF4B | pre-mRNA processing factor 4B | NM_003913.5 | 99.8% | 99.5% | (many diffs) |
| 2 | human | 8899 | PRPF4B | pre-mRNA processing factor 4B | XM_017011410.2 | 99.8% | 99.5% | (many diffs) |
| 3 | human | 8899 | PRPF4B | pre-mRNA processing factor 4B | XM_017011411.2 | 99.8% | 99.5% | (many diffs) |
| 4 | human | 8899 | PRPF4B | pre-mRNA processing factor 4B | XM_017011413.2 | 99.8% | 99.5% | (many diffs) |
| 5 | human | 8899 | PRPF4B | pre-mRNA processing factor 4B | XM_017011414.2 | 99.8% | 99.5% | (many diffs) |
| 6 | human | 8899 | PRPF4B | pre-mRNA processing factor 4B | XM_017011415.2 | 99.8% | 99.5% | (many diffs) |
| 7 | human | 8899 | PRPF4B | pre-mRNA processing factor 4B | XM_017011416.2 | 99.8% | 99.5% | (many diffs) |
| 8 | human | 8899 | PRPF4B | pre-mRNA processing factor 4B | XM_017011417.2 | 99.8% | 99.5% | (many diffs) |
| 9 | human | 8899 | PRPF4B | pre-mRNA processing factor 4B | XM_017011418.2 | 99.8% | 99.5% | (many diffs) |
| 10 | human | 8899 | PRPF4B | pre-mRNA processing factor 4B | XM_017011420.2 | 99.8% | 99.5% | (many diffs) |
| 11 | human | 8899 | PRPF4B | pre-mRNA processing factor 4B | XM_017011421.2 | 99.8% | 99.5% | (many diffs) |
| 12 | human | 8899 | PRPF4B | pre-mRNA processing factor 4B | XM_017011422.2 | 99.8% | 99.5% | (many diffs) |
| 13 | human | 8899 | PRPF4B | pre-mRNA processing factor 4B | XM_017011423.2 | 99.8% | 99.5% | (many diffs) |
| 14 | human | 8899 | PRPF4B | pre-mRNA processing factor 4B | XM_017011424.2 | 99.8% | 99.5% | (many diffs) |
| 15 | human | 8899 | PRPF4B | pre-mRNA processing factor 4B | XM_017011425.2 | 99.8% | 99.5% | (many diffs) |
| 16 | human | 8899 | PRPF4B | pre-mRNA processing factor 4B | XM_017011426.2 | 99.8% | 99.5% | (many diffs) |
| 17 | human | 8899 | PRPF4B | pre-mRNA processing factor 4B | XM_017011427.2 | 99.8% | 99.5% | (many diffs) |
| 18 | human | 8899 | PRPF4B | pre-mRNA processing factor 4B | XM_017011428.2 | 99.8% | 99.5% | (many diffs) |
| 19 | human | 8899 | PRPF4B | pre-mRNA processing factor 4B | XM_017011430.2 | 99.8% | 99.5% | (many diffs) |
| 20 | human | 8899 | PRPF4B | pre-mRNA processing factor 4B | XM_017011432.2 | 85.5% | 84.9% | (many diffs) |
| 21 | human | 8899 | PRPF4B | pre-mRNA processing factor 4B | XM_017011433.2 | 85.5% | 84.9% | (many diffs) |
| 22 | human | 8899 | PRPF4B | pre-mRNA processing factor 4B | XM_017011434.2 | 85.5% | 84.9% | (many diffs) |
| 23 | human | 8899 | PRPF4B | pre-mRNA processing factor 4B | XM_017011435.2 | 85.5% | 84.9% | (many diffs) |
| 24 | human | 8899 | PRPF4B | pre-mRNA processing factor 4B | NR_146784.2 | 84.1% | (many diffs) | |
| 25 | human | 8899 | PRPF4B | pre-mRNA processing factor 4B | XM_011514970.3 | 81.7% | 81.3% | (many diffs) |
| 26 | human | 8899 | PRPF4B | pre-mRNA processing factor 4B | NR_146783.2 | 81.1% | (many diffs) | |
| 27 | human | 8899 | PRPF4B | pre-mRNA processing factor 4B | NR_146785.2 | 79.5% | (many diffs) | |
| 28 | mouse | 19134 | Prpf4b | pre-mRNA processing factor 4B | NM_013830.2 | 88.8% | 94.7% | (many diffs) |
| 29 | mouse | 19134 | Prpf4b | pre-mRNA processing factor 4B | XM_017315425.1 | 88.8% | 94.7% | (many diffs) |
| 30 | mouse | 19134 | Prpf4b | pre-mRNA processing factor 4B | XM_017315426.1 | 88.8% | 94.7% | (many diffs) |
| 31 | mouse | 19134 | Prpf4b | pre-mRNA processing factor 4B | XM_017315427.1 | 88.8% | 94.7% | (many diffs) |
| 32 | mouse | 19134 | Prpf4b | pre-mRNA processing factor 4B | XM_017315428.1 | 88.8% | 94.7% | (many diffs) |
| 33 | mouse | 19134 | Prpf4b | pre-mRNA processing factor 4B | XM_017315429.1 | 88.8% | 94.7% | (many diffs) |
| 34 | mouse | 19134 | Prpf4b | pre-mRNA processing factor 4B | XM_017315430.1 | 88.8% | 94.7% | (many diffs) |
| 35 | mouse | 19134 | Prpf4b | pre-mRNA processing factor 4B | XM_017315431.1 | 88.8% | 94.7% | (many diffs) |
| 36 | mouse | 19134 | Prpf4b | pre-mRNA processing factor 4B | XM_017315432.1 | 88.8% | 94.7% | (many diffs) |
| 37 | mouse | 19134 | Prpf4b | pre-mRNA processing factor 4B | XM_017315433.1 | 88.8% | 94.7% | (many diffs) |
| 38 | mouse | 19134 | Prpf4b | pre-mRNA processing factor 4B | XM_017315434.1 | 88.8% | 94.7% | (many diffs) |
| 39 | mouse | 19134 | Prpf4b | pre-mRNA processing factor 4B | XM_017315435.1 | 88.8% | 94.7% | (many diffs) |
| 40 | mouse | 19134 | Prpf4b | pre-mRNA processing factor 4B | XM_017315436.1 | 88.8% | 94.7% | (many diffs) |
| 41 | mouse | 19134 | Prpf4b | pre-mRNA processing factor 4B | XM_017315437.1 | 88.8% | 94.7% | (many diffs) |
| 42 | mouse | 19134 | Prpf4b | pre-mRNA processing factor 4B | XM_006516582.3 | 77.4% | 79.4% | (many diffs) |
| 43 | mouse | 19134 | Prpf4b | pre-mRNA processing factor 4B | XM_006516583.3 | 77.2% | 79.5% | (many diffs) |
| 44 | mouse | 19134 | Prpf4b | pre-mRNA processing factor 4B | XM_006516584.3 | 77% | 79.9% | (many diffs) |
| 45 | mouse | 19134 | Prpf4b | pre-mRNA processing factor 4B | XM_017315438.1 | 77% | 79.9% | (many diffs) |
| 46 | mouse | 19134 | Prpf4b | pre-mRNA processing factor 4B | XM_017315439.1 | 56.1% | 59.5% | (many diffs) |
| 47 | mouse | 19134 | Prpf4b | pre-mRNA processing factor 4B | XM_017315440.1 | 56.1% | 59.5% | (many diffs) |
| 48 | mouse | 19134 | Prpf4b | pre-mRNA processing factor 4B | XM_017315441.1 | 54.6% | 56.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 3087
- ORF length:
- 3021
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc cgccgcggag acccagtcgc tacgggagca gccagagatg gaagatgcta 121 attctgaaaa gagtataaat gaagaaaatg gagaagtatc agaagaccag tctcaaaata 181 agcacagtcg tcacaaaaaa aagaagcata aacacagaag taaacataag aaacataaac 241 attcctcaga agaagacaag gataaaaaac ataaacataa gcataaacat aagaaacaca 301 aaagaaaaga ggttattgat gcttctgata aagagggtat gtctccagca aaaagaacta 361 aacttgatga tttagctttg ctagaagact tggaaaaaca gagagccttg attaaggccg 421 aacttgataa tgagttaatg gaaggaaagg tccagtctgg tatggggctc attttgcaag 481 gttatgagtc tggctctgaa gaagaggggg aaattcatga aaaggcaaga aatggaaata 541 ggtctagtac tagatcttca agtacaaagg ggaaacttga acttgtggac aataaaatta 601 ctacaaagaa acgaagtaaa agcagatcca aagaacggac tagacatagg tctgataaaa 661 agaaaagtaa ggggggtatt gaaatcgtta aagagaaaac aactaggagc aagtcaaagg 721 agaggaaaaa atctaaaagc ccatccaaaa gaagtaagtc tcaagatcaa gcaaggaaat 781 caaaatcccc tacccttaga aggcgatctc aagagaaaat tggtaaggcc agatctccta 841 ctgatgataa ggttaaaatt gaagataaaa gtaaatcaaa agataggaaa aaatccccaa 901 ttataaatga aagtagaagt cgcgatcgag gtaaaaaatc cagatcccca gttgatttaa 961 gaggtaaatc caaagacaga aggtcacggt ccaaagagag aaaatcaaaa cggtctgaaa 1021 ctgataaaga aaagaagcca attaaatctc cctctaaaga tgcttcatct gggaaagaaa 1081 ataggtcacc cagcagaaga cctggtcgta gtcctaaaag aagaagtttg tctccaaaac 1141 cacgtgataa atcaagaaga agcaggtctc cacttttgaa tgatagaaga tctaagcaga 1201 gcaaataccc ctcgcggaca ctgtctcctg ggagaagagc caagagccga tccttagaaa 1261 gaaaacgacg agaaccagag aggagacgac tttcttctcc aagaacacga cctcgagatg 1321 atatcctcag tagacgtgaa agatcaaaag atgccagccc catcaataga tggtctccaa 1381 cccgaagaag aagtagatct cccattagaa ggaggtctcg ttccccactc agacgtagca 1441 ggtctccaag aagaagaagc agatctactc ggagaaggga cagaggtcgg aggagcagat 1501 cacgcttgcg aaggcggtct cgatcacgcg gtggtcgtag acgaaggagc agaagcaaag 1561 taaaggaaga taaatttaaa ggaagtcttt ctgaaggaat gaaagttgag caggaatctt 1621 cgtctgatga taaccttgaa gactttgatg tagaggaaga agatgaagaa gccctaatag 1681 aacagagaag aatccaaagg caggcaattg ttcagaaata taaatacctt gctgaagata 1741 gcaacatgtc tgtgccatct gaaccaagca gcccccagag cagtacgaga acacgatcac 1801 catctccaga tgacattctg gagcgagtag ctgctgatgt taaagagtat gaacgggaaa 1861 atgttgatac atttgaggcc tcagtgaaag ccaagcataa tctaatgaca gttgaacaga 1921 ataatggttc atctcagaag aagttgttgg cacctgatat gtttacagaa tctgatgata 1981 tgtttgctgc gtattttgat agtgctcgtc ttcgggccgc tggcattgga aaagatttca 2041 aagagaatcc caacctcaga gataactgga ccgatgcaga aggctattat cgtgtgaaca 2101 taggtgaagt cctagataaa cgttacaatg tgtatggcta cactgggcaa ggtgtattca 2161 gtaatgttgt acgagccaga gataatgcaa gagccaacca agaagtggct gtaaagatca 2221 tcagaaacaa tgagctcatg caaaagactg gtttaaaaga attagagttc ttgaaaaaac 2281 ttaatgatgc tgatcctgat gacaaatttc attgtctgag actcttaagg cacttctatc 2341 acaagcagca tctttgtctg gtattcgagc ctctcagcat gaacttacga gaggtgttaa 2401 aaaaatatgg taaagatgtt ggtcttcata ttaaagctgt aagatcctat agtcagcagt 2461 tgttcctggc attgaaactc cttaaaagat gcaatatcct acatgcagat atcaagccag 2521 acaatatcct ggttaatgaa tccaaaacta ttttaaagct ttgcgatttt gggtcggctt 2581 cacatgttgc ggataatgac ataacacctt atcttttcag tagattttat cgtgctcctg 2641 aaatcattat aggtaaaagc tatgactatg gtatagatat gtggtctgta ggttgcacct 2701 tatacgaact ctatactgGA AAAATTTTAT TCCCTGGCAA AACCAATAAC CATATGCTGA 2761 AGCTTGCAAT GGATCTCAAA GGAAAGATGC CAAATAAGAT GATTCGAAAA GGTGTGTTCA 2821 AAGATCAGCA TTTTGATCAA AATCTCAACT TCATGTACAT AGAAGTTGAT AAAGTAACAG 2881 AGAGGGAGAA AGTTACTGTT ATGAGCACCA TTAATCCAAC TAAGGACCTG TTGGCTGACT 2941 TGATTGGGTG CCAGAGACTT CCTGAAGACC AACGTAAGAA AGTACACCAG CTAAAGGACT 3001 TGTTGGACCA GATTCTGATG TTGGACCCAG CTAAACGAAT TAGCATCAAC CAGGCCCTAC 3061 AGCACGCCTT CATCCAGGAA AAAATTTACC CAACTTTCTT GTACAAAGTG GTTGATATCG 3121 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 3181 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GAGATGGTCC 3241 CCGTGCCAAT GTATCAACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 3301 aagatt