Construct: ORF TRCN0000477223
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010308.1_s317c1
- Derived from:
- ccsbBroadEn_03574
- DNA Barcode:
- TGTCTGGTCTCAACTGTCAGACCT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- FBXW7 (55294)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477223
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55294 | FBXW7 | F-box and WD repeat domain ... | NM_001349798.2 | 100% | 100% | |
| 2 | human | 55294 | FBXW7 | F-box and WD repeat domain ... | NM_033632.3 | 100% | 100% | |
| 3 | human | 55294 | FBXW7 | F-box and WD repeat domain ... | XM_011532084.2 | 100% | 100% | |
| 4 | human | 55294 | FBXW7 | F-box and WD repeat domain ... | XM_011532085.2 | 100% | 100% | |
| 5 | human | 55294 | FBXW7 | F-box and WD repeat domain ... | XM_024454121.1 | 100% | 100% | |
| 6 | human | 55294 | FBXW7 | F-box and WD repeat domain ... | XM_024454122.1 | 100% | 100% | |
| 7 | human | 55294 | FBXW7 | F-box and WD repeat domain ... | XM_024454123.1 | 100% | 100% | |
| 8 | human | 55294 | FBXW7 | F-box and WD repeat domain ... | XM_024454124.1 | 100% | 100% | |
| 9 | human | 55294 | FBXW7 | F-box and WD repeat domain ... | NM_018315.5 | 80.8% | 71.9% | (many diffs) |
| 10 | human | 55294 | FBXW7 | F-box and WD repeat domain ... | XM_011532086.2 | 80.8% | 77.8% | 0_1ins262;240_417del |
| 11 | human | 55294 | FBXW7 | F-box and WD repeat domain ... | XM_011532087.2 | 80.8% | 77.8% | 0_1ins262;240_417del |
| 12 | human | 55294 | FBXW7 | F-box and WD repeat domain ... | XM_024454125.1 | 80.8% | 77.8% | 0_1ins262;240_417del |
| 13 | human | 55294 | FBXW7 | F-box and WD repeat domain ... | NM_001013415.2 | 80.6% | 75% | (many diffs) |
| 14 | human | 55294 | FBXW7 | F-box and WD repeat domain ... | XM_011532088.2 | 76.3% | 76.3% | 0_1ins501 |
| 15 | human | 55294 | FBXW7 | F-box and WD repeat domain ... | XM_024454126.1 | 76.3% | 76.3% | 0_1ins501 |
| 16 | human | 55294 | FBXW7 | F-box and WD repeat domain ... | NM_001257069.1 | 24% | 23.7% | (many diffs) |
| 17 | mouse | 50754 | Fbxw7 | F-box and WD-40 domain prot... | NM_001177773.1 | 90.7% | 96% | (many diffs) |
| 18 | mouse | 50754 | Fbxw7 | F-box and WD-40 domain prot... | NM_001177774.1 | 90.7% | 96% | (many diffs) |
| 19 | mouse | 50754 | Fbxw7 | F-box and WD-40 domain prot... | XM_006501656.3 | 90.7% | 96% | (many diffs) |
| 20 | mouse | 50754 | Fbxw7 | F-box and WD-40 domain prot... | XM_006501657.3 | 90.7% | 96% | (many diffs) |
| 21 | mouse | 50754 | Fbxw7 | F-box and WD-40 domain prot... | NM_080428.3 | 75.8% | 71.3% | (many diffs) |
| 22 | mouse | 50754 | Fbxw7 | F-box and WD-40 domain prot... | XM_006501658.2 | 75.1% | 75.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2190
- ORF length:
- 2121
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gaatcaggaa ctgctctctg tgggcagcaa aagacgacga actggaggct 121 ctctgagagg taacccttcc tcaagccagg tagatgaaga acagatgaat cgtgtggtag 181 aggaggaaca gcaacagcaa ctcagacaac aagaggagga gcacactgca aggaatggtg 241 aagttgttgg agtagaacct agacctggag gccaaaatga ttcccagcaa ggacagttgg 301 aagaaaacaa taatagattt atttcggtag atgaggactc ctcaggaaac caagaagaac 361 aagaggaaga tgaagaacat gctggtgaac aagatgagga ggatgaggag gaggaggaga 421 tggaccagga gagtgacgat tttgatcagt ctgatgatag tagcagagaa gatgaacata 481 cacatactaa cagtgtcacg aactccagta gtattgtgga cctgcccgtt caccaactct 541 cctccccatt ctatacaaaa acaacaaaaa tgaaaagaaa gttggaccat ggttctgagg 601 tccgctcttt ttctttggga aagaaaccat gcaaagtctc agaatataca agtaccactg 661 ggcttgtacc atgttcagca acaccaacaa cttttgggga cctcagagca gccaatggcc 721 aagggcaaca acgacgccga attacatctg tccagccacc tacaggcctc caggaatggc 781 taaaaatgtt tcagagctgg agtggaccag agaaattgct tgctttagat gaactcattg 841 atagttgtga accaacacaa gtaaaacata tgatgcaagt gatagaaccc cagtttcaac 901 gagacttcat ttcattgctc cctaaagagt tggcactcta tgtgctttca ttcctggaac 961 ccaaagacct gctacaagca gctcagacat gtcgctactg gagaattttg gctgaagaca 1021 accttctctg gagagagaaa tgcaaagaag aggggattga tgaaccattg cacatcaaga 1081 gaagaaaagt aataaaacca ggtttcatac acagtccatg gaaaagtgca tacatcagac 1141 agcacagaat tgatactaac tggaggcgag gagaactcaa atctcctaag gtgctgaaag 1201 gacatgatga tcatgtgatc acatgcttac agttttgtgg taaccgaata gttagtggtt 1261 ctgatgacaa cactttaaaa gtttggtcag cagtcacagg caaatgtctg agaacattag 1321 tgggacatac aggtggagta tggtcatcac aaatgagaga caacatcatc attagtggat 1381 ctacagatcg gacactcaaa gtgtggaatg cagagactgg agaatgtata cacaccttat 1441 atgggcatac ttccactgtg cgttgtatgc atcttcatga aaaaagagtt gttagcggtt 1501 ctcgagatgc cactcttagg gtttgggata ttgagacagg ccagtgttta catgttttga 1561 tgggtcatgt tgcagcagtc cgctgtgttc aatatgatgg caggagggtt gttagtggag 1621 catatgattt tatggtaaag gtgtgggatc cagagactga aacctgtcta cacacgttgc 1681 aggggcatac taatagagtc tattcattac agtttgatgg tatccatgtg gtgagtggat 1741 CTCTTGATAC ATCAATCCGT GTTTGGGATG TGGAGACAGG GAATTGCATT CACACGTTAA 1801 CAGGGCACCA GTCGTTAACA AGTGGAATGG AACTCAAAGA CAATATTCTT GTCTCTGGGA 1861 ATGCAGATTC TACAGTTAAA ATCTGGGATA TCAAAACAGG ACAGTGTTTA CAAACATTGC 1921 AAGGTCCCAA CAAGCATCAG AGTGCTGTGA CCTGTTTACA GTTCAACAAG AACTTTGTAA 1981 TTACCAGCTC AGATGATGGA ACTGTAAAAC TATGGGACTT GAAAACGGGT GAATTTATTC 2041 GAAACCTAGT CACATTGGAG AGTGGGGGGA GTGGGGGAGT TGTGTGGCGG ATCAGAGCCT 2101 CAAACACAAA GCTGGTGTGT GCAGTTGGGA GTCGGAATGG GACTGAAGAA ACCAAGCTGC 2161 TGGTGCTGGA CTTTGATGTG GACATGAAGT TGCCAACTTT CTTGTACAAA GTGGTTGATA 2221 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 2281 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGATGTCT 2341 GGTCTCAACT GTCAGACCTA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 2401 tgaaagatt