Transcript: Mouse XM_006501656.3

PREDICTED: Mus musculus F-box and WD-40 domain protein 7 (Fbxw7), transcript variant X1, mRNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Fbxw7 (50754)
Length:
7737
CDS:
3948..6080

Additional Resources:

NCBI RefSeq record:
XM_006501656.3
NBCI Gene record:
Fbxw7 (50754)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_006501656.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000365897 TTCAACAAGAACTTCGTAATT pLKO_005 5850 CDS 100% 13.200 18.480 N Fbxw7 n/a
2 TRCN0000373991 ACTTCTACTGTACGGTGTATG pLKO_005 5337 CDS 100% 10.800 15.120 N Fbxw7 n/a
3 TRCN0000365828 ATCCGAAACCTCGTCACATTG pLKO_005 5925 CDS 100% 10.800 15.120 N Fbxw7 n/a
4 TRCN0000012832 CGCATAGTTAGTGGTTCTGAT pLKO.1 5133 CDS 100% 4.950 6.930 N Fbxw7 n/a
5 TRCN0000012831 CGGAGGATTACATCTGTCCAA pLKO.1 4623 CDS 100% 2.640 3.696 N Fbxw7 n/a
6 TRCN0000006555 CCTAAAGAGTTGGCACTCTAT pLKO.1 4809 CDS 100% 0.495 0.693 N FBXW7 n/a
7 TRCN0000365827 CCCATGGAAGAGTGCGTATAT pLKO_005 5003 CDS 100% 13.200 9.240 N Fbxw7 n/a
8 TRCN0000365826 CTCTCAGGGTTTGGGATATTG pLKO_005 5401 CDS 100% 13.200 9.240 N Fbxw7 n/a
9 TRCN0000373989 TGTTAGTGGAGCTTATGATTT pLKO_005 5498 CDS 100% 13.200 9.240 N Fbxw7 n/a
10 TRCN0000373990 CAGCACAGAATTGATACAAAC pLKO_005 5028 CDS 100% 10.800 7.560 N Fbxw7 n/a
11 TRCN0000012828 CCAGTCATTAACGAGTGGAAT pLKO.1 5696 CDS 100% 4.950 3.465 N Fbxw7 n/a
12 TRCN0000012830 CCAGTGTTTACACGTCCTGAT pLKO.1 5429 CDS 100% 4.050 2.835 N Fbxw7 n/a
13 TRCN0000235423 TGATGGCAGGAGGGTTGTTAG pLKO_005 5483 CDS 100% 10.800 6.480 N FBXW7 n/a
14 TRCN0000166364 CACACACACACACACACACAA pLKO.1 1305 5UTR 100% 4.950 2.475 Y KAAG1 n/a
15 TRCN0000087583 AGGAGGAAGAGGAAGAGGAAA pLKO.1 4282 CDS 100% 4.950 2.475 Y Adam32 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_006501656.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_03574 pDONR223 100% 90.7% 96% None (many diffs) n/a
2 ccsbBroad304_03574 pLX_304 0% 90.7% 96% V5 (many diffs) n/a
3 TRCN0000477223 TGTCTGGTCTCAACTGTCAGACCT pLX_317 21.4% 90.7% 96% V5 (many diffs) n/a
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