Transcript: Human NM_018315.5

Homo sapiens F-box and WD repeat domain containing 7 (FBXW7), transcript variant 2, mRNA.

Source:
NCBI, updated 2019-08-21
Taxon:
Homo sapiens (human)
Gene:
FBXW7 (55294)
Length:
4317
CDS:
97..1980

Additional Resources:

NCBI RefSeq record:
NM_018315.5
NBCI Gene record:
FBXW7 (55294)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_018315.5, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000235424 GGCAACAACGACGCCGAATTA pLKO_005 512 CDS 100% 13.200 18.480 N FBXW7 n/a
2 TRCN0000235420 GGCATACTAATAGAGTCTATT pLKO_005 1472 CDS 100% 13.200 18.480 N FBXW7 n/a
3 TRCN0000006557 CCAGTCGTTAACAAGTGGAAT pLKO.1 1596 CDS 100% 4.950 6.930 N FBXW7 n/a
4 TRCN0000006555 CCTAAAGAGTTGGCACTCTAT pLKO.1 709 CDS 100% 0.495 0.693 N FBXW7 n/a
5 TRCN0000368359 TCAAACCAGGTGCAATTATTT pLKO_005 2257 3UTR 100% 15.000 12.000 N FBXW7 n/a
6 TRCN0000235422 ATGGGTTTCTACGGCACATTA pLKO_005 319 CDS 100% 13.200 10.560 N FBXW7 n/a
7 TRCN0000365964 ATGGGTTTCTACGGCACATTA pLKO_005 319 CDS 100% 13.200 10.560 N Fbxw7 n/a
8 TRCN0000355643 AGTTTCAACGAGACTTCATTT pLKO_005 680 CDS 100% 13.200 9.240 N FBXW7 n/a
9 TRCN0000235421 CCAATTGTGTAGACGATATAC pLKO_005 2003 3UTR 100% 13.200 9.240 N FBXW7 n/a
10 TRCN0000355641 CTCTTAGGGTTTGGGATATTG pLKO_005 1301 CDS 100% 13.200 9.240 N FBXW7 n/a
11 TRCN0000355639 TGCCTGAGCATGTCCACATTA pLKO_005 193 CDS 100% 13.200 9.240 N FBXW7 n/a
12 TRCN0000355642 ACTTCCACTGTGCGTTGTATG pLKO_005 1237 CDS 100% 10.800 7.560 N FBXW7 n/a
13 TRCN0000006556 CCAGAGAAATTGCTTGCTTTA pLKO.1 595 CDS 100% 10.800 7.560 N FBXW7 n/a
14 TRCN0000355640 TGATACATCAATCCGTGTTTG pLKO_005 1533 CDS 100% 10.800 7.560 N FBXW7 n/a
15 TRCN0000235423 TGATGGCAGGAGGGTTGTTAG pLKO_005 1383 CDS 100% 10.800 7.560 N FBXW7 n/a
16 TRCN0000006558 CCAGAGACTGAAACCTGTCTA pLKO.1 1438 CDS 100% 4.950 3.465 N FBXW7 n/a
17 TRCN0000355644 CTAGAAAGGAACTGCAATAAT pLKO_005 2375 3UTR 100% 15.000 9.000 N FBXW7 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_018315.5, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_03574 pDONR223 100% 80.8% 71.9% None (many diffs) n/a
2 ccsbBroad304_03574 pLX_304 0% 80.8% 71.9% V5 (many diffs) n/a
3 TRCN0000477223 TGTCTGGTCTCAACTGTCAGACCT pLX_317 21.4% 80.8% 71.9% V5 (many diffs) n/a
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